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Application of PNO1 inhibitor to preparation of esophagus cancer treatment medicines

A treatment drug and technology for esophageal cancer, applied in the field of biomedicine, can solve problems such as the limitation of chemotherapy efficacy

Active Publication Date: 2020-01-07
THE FIRST AFFILIATED HOSPITAL OF BENGBU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficacy of chemotherapy is limited due to reasons such as primary and acquired drug resistance due to individual differences in patients.

Method used

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  • Application of PNO1 inhibitor to preparation of esophagus cancer treatment medicines
  • Application of PNO1 inhibitor to preparation of esophagus cancer treatment medicines
  • Application of PNO1 inhibitor to preparation of esophagus cancer treatment medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] (1) Experimental method

[0131] 1. Target cells

[0132] Human esophageal cancer cells: Eca-109, TE-1.

[0133] 2. PNO1 gene expression in esophageal cancer cells

[0134] 2.1 Experimental method:

[0135] Primer design for the PNO1 gene. The primer sequences are 5'-TGTTAAACCCCTAAAGGGAGACC-3' (SEQ ID NO: 1) and 5'-CCTTGTCCGTGTCACATTCTCT-3' (SEQ ID NO: 2) to extract Eca-109, TE-1, EC9706 cells, extract RNA respectively, reverse transcription The cDNA was obtained, and the mRNA expression of PNO1 in ECA-109, TE-1, and EC9706 cells was detected by RT-PCR with GAPDH as an internal reference.

[0136] 2.2 Experimental steps

[0137] 2.2.1 Total RNA extraction

[0138] (1) Collect the cells, centrifuge at 2000rpm for 5min, remove the supernatant, add 1mL Trizol to the cell pellet, mix thoroughly, let stand at room temperature for 5min, and then transfer to a new 1.5mL EP tube.

[0139] (2) Add 200 μL of chloroform to each tube, turn the EP tube upside down for 15 seco...

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Abstract

The invention relates to an application of a PNO1 inhibitor to preparation of esophagus cancer treatment medicines, belongs to the field of biopharmaceutical research, and particularly relates to an application of a PNO1 inhibitor to preparation of esophagus cancer treatment medicines. The inventor firstly finds that the PNO1 can be used as an esophagus cancer treatment target point. The PNO1 inhibitor can restrain the proliferation rate of esophagus cancer cells, can restrain the cloning and forming capacity of the esophagus cancer cells, can promote esophagus cancer apoptosis, can restrain esophagus cancer cell migration, can restrain esophagus cancer cell transfer, and can restrain esophagus cancer cell invasion and transfer, so as to treat esophagus cancer, and a new direction is opened up for treatment of esophagus cancer.

Description

technical field [0001] The invention belongs to the field of biomedical research, and in particular relates to the use of PNO1 inhibitors in the preparation of drugs for treating esophageal cancer. Background technique [0002] Esophageal cancer (Esophageal cancer, EC) is a common malignant tumor of the digestive tract. Globally, the mortality rate of esophageal cancer ranks sixth. Esophageal cancer is divided into two main pathological types, namely esophageal adenocarcinoma and esophageal squamous cell carcinoma. At present, the effective treatment of esophageal cancer mainly relies on early surgery, and its 5-year survival rate is still less than 10%. Chemotherapy has been widely used clinically as a routine treatment after tumor resection and radiotherapy for advanced esophageal cancer. However, due to individual differences in patients leading to primary and acquired drug resistance and other reasons, the efficacy of chemotherapy is limited. [0003] Feasible therape...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/867A61K45/00A61P35/00
CPCA61K45/00A61P35/00C12N15/113C12N15/867C12N2310/14C12N2310/141C12N2740/15043
Inventor 汪国文陶涛李其才王祖义
Owner THE FIRST AFFILIATED HOSPITAL OF BENGBU MEDICAL COLLEGE
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