Application of S-methylisothiourea sulfate in preparation of anti-influenza virus and escherichia coli co-infection drugs

A methylisothiourea sulfate, anti-influenza virus technology, applied in the direction of antiviral agents, antibacterial drugs, resistance to vector-borne diseases, etc., can solve the problems of easy invasion of Escherichia coli, respiratory system damage, secondary infection , to achieve the effect of reducing the viral and bacterial load in the lungs, enhancing the resistance and improving the survival rate

Inactive Publication Date: 2021-05-07
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liu Xiangmin and others investigated the mixed infection of chicken-derived E. coli and low-pathogenic H9N2 subtype avian influenza virus in Shandong Province, and found that the phenomenon of mixed infection of H9N2 subtype avian influenza virus and E. coli is relatively common clinically, and the first and second The detection rate is higher in the fourth quarter, which may be due to the high incidence of influenza in winter and spring, which causes damage to the respiratory system, making it easy for E. coli to invade and cause secondary infection

Method used

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  • Application of S-methylisothiourea sulfate in preparation of anti-influenza virus and escherichia coli co-infection drugs
  • Application of S-methylisothiourea sulfate in preparation of anti-influenza virus and escherichia coli co-infection drugs
  • Application of S-methylisothiourea sulfate in preparation of anti-influenza virus and escherichia coli co-infection drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: The compound S-methylisothiourea sulfate can inhibit the proliferation of Escherichia coli in the case of mixed infection at the cellular level

[0028] Inoculate an appropriate amount of MLE12 cells into 9 T25 cell flasks, and infect the H9N2 influenza virus when the confluence of the cells is 90%-100%. Wash the cells 2-3 times with PBS, then add 2 mL of virus maintenance solution (2 μg / mL trypsin in DMEM without serum and double antibody), and add 30 μL of H9N2 subtype influenza virus (MOI=1) to 6 cell flasks. Then place it in a cell culture incubator at 37° C., saturated humidity, and 5% CO2 concentration for 1 hour, during which the cell bottle is shaken every 15 minutes. After 1 hour, the virus maintenance solution was discarded, and the cells were washed again with PBS for 3 times, and 8ml of fresh virus maintenance solution without double antibody was added to the cell bottle, and the final concentration of 1mM S was added to three of the cell bottles ...

Embodiment 2

[0029] Example 2: Compound S-methylisothiouronium sulfate (SMT) can enhance the resistance of mice to the mixed infection of H9N2 subtype influenza virus and Escherichia coli

[0030] 1. Grouping and handling of test animals

[0031] Select 60 SPF grade BALB / c female mice aged 6 weeks (purchased from Shanghai Slack Experimental Animal Co., Ltd.), and randomly divide them into 3 groups, which are normal non-challenge group (control group); H9N2 subtype influenza virus and large intestine Bacillus mixed infection-S-methylisothiouronium sulfate untreated group (test group I); H9N2 subtype influenza virus and Escherichia coli mixed infection-S-methylisothiourea sulfate treatment group (test II group), 20 in each group. The mice in the test group I and the test group II were pretreated with ultrapure water and S-methylisothiouronium sulfate (20 mg / kg / mouse) for 24 hours respectively, and then each group was given nasal drops of H9N2 subtype influenza virus (5× 104PFU) and Escheri...

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Abstract

The invention discloses application of S-methylisothiourea sulfate in preparation of anti-influenza virus and escherichia coli co-infection drugs. Experiments prove that at the cellular level, the compound S-methylisothiourea sulfate can significantly inhibit proliferation of escherichia coli under the condition of mixed infection; at the animal level, after S-methylisothiourea sulfate (20mg/kg/mouse) is dropped into a mouse in a nasal dropping mode, the resistance of the mouse to H9N2 subtype influenza virus and escherichia coli mixed infection is enhanced, the survival rate of the mouse infected with H9N2 subtype influenza virus and escherichia coli is increased compared with that of a mouse infected with H9N2 subtype influenza virus and escherichia coli without dropping S-methylisothiourea sulfate, and the carrying capacity of influenza virus and escherichia coli in the lung is obviously reduced. The compound is effectively applied to resisting influenza virus and escherichia coli mixed infection, can serve as a new candidate drug for resisting influenza virus and escherichia coli mixed infection, and provides more choices for treatment of influenza virus and escherichia coli mixed infection clinically.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to the application of a compound in the preparation of anti-influenza virus and E. Bacillus co-infection drug application. Background technique [0002] Avian influenza virus (AIV) belongs to type A influenza virus, which can cause various symptoms of poultry from respiratory system to severe systemic sepsis; avian colibacillosis is caused by pathogenic Escherichia coli of various serotypes General term for different types of poultry diseases. Including Escherichia coli air sacculitis, sepsis, omphalitis, salpingitis, peritonitis and Escherichia coli granuloma, etc., which are seriously harmful to the poultry industry. Colibacillosis is a conditional disease, and poor feeding management, stress or concurrent infection with other pathogens can all be the cause of colibacillosis. Chickens of all ages can be infected with colibacillosis, and the morbidity and mortality are affected by vario...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/17A61P31/16A61P31/04
CPCA61K31/17A61P31/16A61P31/04Y02A50/30
Inventor 王松陈吉龙池晓娟江宁
Owner FUJIAN AGRI & FORESTRY UNIV
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