Method for simultaneously determining five types of antibiotic residues in water sample

A technology for antibiotics and residues, applied in the field of analysis, can solve the problems of limited species, long time-consuming antibiotic residues, insufficient sensitivity, etc., and achieve the effects of low detection limit and quantification limit, high recovery rate and good repeatability.

Pending Publication Date: 2020-01-07
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, provide a kind of method for simultaneously measuring five types of antibiotic residues in water samples, to overcome the time-co

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously determining five types of antibiotic residues in water sample
  • Method for simultaneously determining five types of antibiotic residues in water sample
  • Method for simultaneously determining five types of antibiotic residues in water sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Present embodiment 1 provides a kind of method of simultaneously measuring five classes of antibiotic residues in water sample, comprises the steps:

[0043] 1. Water sample pretreatment:

[0044] (1) Pretreatment of water samples: the target river water was collected in a 1L brown plastic bottle, stored in an ice box and brought back to the laboratory. Measure 500mL water sample with a measuring cylinder, collect the filtered water sample through a suction filtration device equipped with a 0.45μm glass fiber filter membrane, adjust the pH to 3.8-4.0, and add 10mL of complexing agent Na with a concentration of 5% v / v 2 -EDTA complexes metal cations in water to prevent the detection rate of target antibiotics from being reduced due to the complexation of metal cations in water with antibiotics such as tetracyclines, quinolones, and sulfonamides;

[0045] (2) Enrichment, purification and concentration of water samples: Pass 5mL methanol naturally through the HLB solid-ph...

Embodiment 2

[0061] Present embodiment 2 provides a kind of method of simultaneously measuring five classes of antibiotic residues in the water sample, comprises the steps:

[0062] 1. Water sample pretreatment:

[0063] (1) Pretreatment of water samples: the target river water was collected in a 1L brown plastic bottle, stored in an ice box and brought back to the laboratory. Measure 1000mL water sample with a graduated cylinder, collect the filtered water sample through a suction filtration device equipped with a 0.45μm glass fiber filter membrane, adjust the pH to 3.8-4.0, and add 10mL of complexing agent Na with a concentration of 5% v / v 2 -EDTA complexes metal cations in water to prevent the detection rate of target antibiotics from being reduced due to the complexation of metal cations in water with antibiotics such as tetracyclines, quinolones, and sulfonamides;

[0064] (2) Enrichment, purification and concentration of water samples:

[0065] With reference to (2) in step 1 in Ex...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for simultaneously determining five types of antibiotic residues in a water sample. The method comprises the following steps of carrying out pre-treating on a to-be-detected water sample, carrying out enrichment and purification treatment on the pre-treated water sample, then carrying out concentration treatment on the to-be-detected water sample after enrichment treatment, preparing the concentrated to-be-tested sample into an antibiotic to-be-detected liquid and carrying out machine detection on the antibiotic to-be-detected liquid, and the like. By adoptionof the method simultaneously determining five types of antibiotic residues in the water sample, five types of antibiotic residues in the water sample can be quantitatively analyzed at the same time, the detection efficiency is high, the detection limit and the quantitative limit are relatively low, the sensitivity is high, and residual trace antibiotic species and content in the water sample can be detected. In addition, the method has the advantages of high recovery rate, high repeatability and bright application prospect.

Description

technical field [0001] The invention belongs to the technical field of analysis, and in particular relates to a method for simultaneously measuring five types of antibiotic residues in water samples. Background technique [0002] Antibiotics are widely used in human and animal disease treatment and daily prevention and control. During the rapid and intensive development of modern China, the abuse of human and veterinary antibiotics is very serious, and the negative impact caused by it cannot be ignored. Studies have shown that the bioavailability of antibiotics is only 10%-60%, resulting in a large amount of antibiotics entering the abiotic environment in the form of the parent and its metabolites. In addition, the massive discharge of solid and liquid waste from residential areas, pharmaceutical factories, hospitals, and farms has caused antibiotics to continue to enter the surface environment in large quantities, and eventually lead to antibiotics becoming a new type of p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/72
Inventor 章程刘崇炫张丽丽连柯汀
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap