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Method for simultaneously determining five types of antibiotic residues in water sample

A technology for antibiotics and residues, applied in the field of analysis, can solve the problems of limited species, long time-consuming antibiotic residues, insufficient sensitivity, etc., and achieve the effects of low detection limit and quantification limit, high recovery rate and good repeatability.

Pending Publication Date: 2020-01-07
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, provide a kind of method for simultaneously measuring five types of antibiotic residues in water samples, to overcome the time-consuming and relatively time-consuming detection of the presence of antibiotic residues by existing high-performance liquid chromatography-tandem mass spectrometry. technical problems of length, insufficient sensitivity and limited variety

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  • Method for simultaneously determining five types of antibiotic residues in water sample
  • Method for simultaneously determining five types of antibiotic residues in water sample
  • Method for simultaneously determining five types of antibiotic residues in water sample

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Embodiment 1

[0042] Present embodiment 1 provides a kind of method of simultaneously measuring five classes of antibiotic residues in water sample, comprises the steps:

[0043] 1. Water sample pretreatment:

[0044] (1) Pretreatment of water samples: the target river water was collected in a 1L brown plastic bottle, stored in an ice box and brought back to the laboratory. Measure 500mL water sample with a measuring cylinder, collect the filtered water sample through a suction filtration device equipped with a 0.45μm glass fiber filter membrane, adjust the pH to 3.8-4.0, and add 10mL of complexing agent Na with a concentration of 5% v / v 2 -EDTA complexes metal cations in water to prevent the detection rate of target antibiotics from being reduced due to the complexation of metal cations in water with antibiotics such as tetracyclines, quinolones, and sulfonamides;

[0045] (2) Enrichment, purification and concentration of water samples: Pass 5mL methanol naturally through the HLB solid-ph...

Embodiment 2

[0061] Present embodiment 2 provides a kind of method of simultaneously measuring five classes of antibiotic residues in the water sample, comprises the steps:

[0062] 1. Water sample pretreatment:

[0063] (1) Pretreatment of water samples: the target river water was collected in a 1L brown plastic bottle, stored in an ice box and brought back to the laboratory. Measure 1000mL water sample with a graduated cylinder, collect the filtered water sample through a suction filtration device equipped with a 0.45μm glass fiber filter membrane, adjust the pH to 3.8-4.0, and add 10mL of complexing agent Na with a concentration of 5% v / v 2 -EDTA complexes metal cations in water to prevent the detection rate of target antibiotics from being reduced due to the complexation of metal cations in water with antibiotics such as tetracyclines, quinolones, and sulfonamides;

[0064] (2) Enrichment, purification and concentration of water samples:

[0065] With reference to (2) in step 1 in Ex...

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Abstract

The invention discloses a method for simultaneously determining five types of antibiotic residues in a water sample. The method comprises the following steps of carrying out pre-treating on a to-be-detected water sample, carrying out enrichment and purification treatment on the pre-treated water sample, then carrying out concentration treatment on the to-be-detected water sample after enrichment treatment, preparing the concentrated to-be-tested sample into an antibiotic to-be-detected liquid and carrying out machine detection on the antibiotic to-be-detected liquid, and the like. By adoptionof the method simultaneously determining five types of antibiotic residues in the water sample, five types of antibiotic residues in the water sample can be quantitatively analyzed at the same time, the detection efficiency is high, the detection limit and the quantitative limit are relatively low, the sensitivity is high, and residual trace antibiotic species and content in the water sample can be detected. In addition, the method has the advantages of high recovery rate, high repeatability and bright application prospect.

Description

technical field [0001] The invention belongs to the technical field of analysis, and in particular relates to a method for simultaneously measuring five types of antibiotic residues in water samples. Background technique [0002] Antibiotics are widely used in human and animal disease treatment and daily prevention and control. During the rapid and intensive development of modern China, the abuse of human and veterinary antibiotics is very serious, and the negative impact caused by it cannot be ignored. Studies have shown that the bioavailability of antibiotics is only 10%-60%, resulting in a large amount of antibiotics entering the abiotic environment in the form of the parent and its metabolites. In addition, the massive discharge of solid and liquid waste from residential areas, pharmaceutical factories, hospitals, and farms has caused antibiotics to continue to enter the surface environment in large quantities, and eventually lead to antibiotics becoming a new type of p...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/72
Inventor 章程刘崇炫张丽丽连柯汀
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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