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Establishment method and detection method of uplc characteristic map of fresh Houttuynia cordata

A characteristic map and method establishment technology, applied in the field of traditional Chinese medicine quality inspection, can solve the problems of chemical composition information differences, unfavorable popularization and application, and large amount of reagents, etc., and achieve the effect of comprehensive quality inspection, good reproducibility, and easy operation

Active Publication Date: 2022-03-11
GUANGDONG YIFANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing characteristic maps for Houttuynia cordata are for dried Houttuynia cordata or dried Houttuynia cordata preparations, and fresh Houttuynia cordata is a species that is different from dried Houttuynia cordata under the item of Houttuynia cordata stipulated in the Chinese Pharmacopoeia Fresh Chinese medicinal materials, compared with dried Houttuynia cordata, fresh Houttuynia cordata is ready-to-use without any processing, the active ingredients can be preserved to the greatest extent, and the chemical composition information reflected in the characteristic map is also different
And at present, the detection of Houttuynia cordata fingerprint (or characteristic) spectrum is all adopted high-performance liquid chromatography (HPLC), which takes a long time (30-90 minutes), consumes a lot of reagents, and has high detection cost, which is not conducive to comprehensive Promote application

Method used

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  • Establishment method and detection method of uplc characteristic map of fresh Houttuynia cordata
  • Establishment method and detection method of uplc characteristic map of fresh Houttuynia cordata
  • Establishment method and detection method of uplc characteristic map of fresh Houttuynia cordata

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Embodiment 1

[0069] The establishment method of the characteristic map of embodiment 1 fresh Houttuynia herba medicinal material

[0070] The steps of the establishment method are as follows:

[0071] 1) Reference substance reference solution preparation: take neochlorogenic acid reference substance, chlorogenic acid reference substance, cryptochlorogenic acid reference substance, rutin reference substance, hyperin reference substance, isoquercitrin reference substance and quercetin An appropriate amount of glycoside reference substance was accurately weighed, and 90% methanol aqueous solution was added to make a reference substance solution containing 50 μg per 1 mL.

[0072] 2) Preparation of the test solution: take 2.0 g of fresh Houttuynia cordata medicinal material, accurately weigh it, put it in a stoppered conical flask, accurately add 50 mL of 90% methanol aqueous solution, seal it tightly, weigh it, heat and reflux for 30 minutes, put Cool, weigh again, make up the lost weight wi...

Embodiment 2

[0155] Embodiment 2 The content determination method of multi-index components of fresh Houttuynia cordata medicinal material

[0156] The steps of the assay method are as follows:

[0157] 1) Preparation of reference substance reference substance solution: take appropriate amount of chlorogenic acid reference substance, hyperin reference substance and quercitrin reference substance, weigh them accurately, add 90% methanol aqueous solution to make a mixed reference substance containing 40 μg per 1 mL substance solution, that is.

[0158] 2) Preparation of the sample solution to be tested: take 2.0 g of fresh Houttuynia cordata medicinal material, accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 50 mL of 90% methanol aqueous solution, seal it tightly, weigh it, heat and reflux for 30 minutes, put Cool, weigh again, make up the lost weight with 90% methanol aqueous solution, shake well, filter, and take the filtrate to obtain the final product.

[01...

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Abstract

The invention relates to a method for establishing a UPLC characteristic map of fresh Houttuynia herba medicinal material and a detection method. The UPLC characteristic map establishment method comprises the following steps: the preparation of the reference substance solution; the preparation of the test solution: take fresh Houttuynia cordata medicinal material, after chopping, add methanol aqueous solution or methanol whose volume fraction is 70~95% to extract, extract Liquid filtration, get continued filtrate, need testing solution; Draw described reference substance solution and need testing solution, inject in ultra-high performance liquid chromatograph and detect, the mobile phase A that described ultra high performance liquid chromatograph adopts is Acetonitrile, mobile phase B is phosphoric acid aqueous solution with a volume fraction of 0.05-0.15%, and the elution method is gradient elution. The UPLC characteristic map establishment method can establish and obtain the characteristic map of fresh Houttuynia cordata medicinal material, which fills the gap in the prior art, and the quality detection is comprehensive and reliable, and takes a short time, which is convenient for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of quality detection of traditional Chinese medicines, in particular to a method for establishing a UPLC characteristic map of fresh Houttuynia cordata medicinal material and a detection method. Background technique [0002] Fresh Houttuynia cordata Thunb. is the fresh whole herb of Houttuynia cordata Thunb., and studies have shown that fresh Houttuynia cordata contains volatile oils, organic acids, flavonoids, phenols, and alkaloids. It has the effects of clearing away heat and detoxifying, eliminating carbuncle and draining pus, diuresis and treating stranguria. It is mainly used for lung abscess, phlegm-heat cough, hot dysentery and stranguria, carbuncle sore and swelling. One of the resources is also a common fresh Chinese medicinal material recorded in the previous editions of the "Chinese Pharmacopoeia", which has broad economic and medicinal value. However, the 2015 edition of the "Chinese Pharmacopo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8631
Inventor 徐杰梁志毅张志鹏魏梅黄梦婷邓李红田清清鲁云程学仁
Owner GUANGDONG YIFANG PHARMA
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