H3N2 and H3N8 subgenetype dog influenza divalent inactivated vaccine and preparation method and application thereof

A technology of H3N2 and H3N8, which is applied in the field of H3N2 and H3N8 subtype canine influenza bivalent inactivated vaccine and its preparation, and can solve the problems of vaccine marketing and other issues

Active Publication Date: 2020-01-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, China has not yet launched relevant vaccines.

Method used

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  • H3N2 and H3N8 subgenetype dog influenza divalent inactivated vaccine and preparation method and application thereof
  • H3N2 and H3N8 subgenetype dog influenza divalent inactivated vaccine and preparation method and application thereof
  • H3N2 and H3N8 subgenetype dog influenza divalent inactivated vaccine and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, the discovery of H3N2 subtype canine influenza virus candidate vaccine strain

[0055] 1. Discovery of new branch H3N2 subtype canine influenza virus strain

[0056] New branch H3N2 subtype canine influenza virus strain A / canine / Beijing / 0528-147 / 2017 Molecular identification results of 147: the sequence has been uploaded to GenBank, sequence number: PB2 (MK212678, 2017 / 05 / 28), PB1 (MK212679, 2017 / 05 / 28), PA(MK212680, 2017 / 05 / 28), HA(MK212685, 2017 / 05 / 28), NP(MK212682, 2017 / 05 / 28), NA(MK212683, 2017 / 05 / 28), M (MK212684, 2017 / 05 / 28), NS (MK212681, 2017 / 05 / 28).

[0057] The virus strain A / canine / Beijing / 0528-147 / 2017 147 was preserved in the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences on April 29, 2019 (address No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing) , the preservation number is CGMCC NO.17589, and the classification is named H3N2 subtype canine influenza virus.

[0058] The molecu...

Embodiment 2

[0078] The preparation of embodiment 2, H3N2 and H3N8 canine influenza virus bivalent inactivated vaccine

[0079] (1) Preparation of H3N2 and H3N8 Canine Influenza Virus Whole Virus Bivalent Inactivated Vaccine

[0080] 1. Screening of vaccine virus strains

[0081] According to the titer detected in Example 1 above, the HA titer of the candidate strain for preparing the whole virus vaccine is too low, so the following passages are carried out:

[0082] The vaccine candidate virus strains A / canine / Beijing / 0528-147 / 2017 147 (H3N2), A / canine / Quanzhou / 1224-1109 / 2018 (H3N2) chicken embryo allantoic fluid and A / canine / Iowa / 13628 / 2005 (H3N8) chicken embryo allantoic fluid were made 10 times -1 ~10 -3 Dilute, inoculate 9-day-old to 11-day-old SPF chicken embryos through the allantoic cavity, the inoculation volume is 0.2mL / embryo, and incubate at 37°C. Aseptically collect the allantoic fluid of chicken embryos from 48h to 96h, measure the hemagglutination titer (HA) with 1% chi...

Embodiment 3

[0244] Example 3, Immunogenicity and protective evaluation of H3N2 canine influenza virus and H3N8 canine influenza virus bivalent inactivated vaccine

[0245] 1. Canine vaccine efficacy test

[0246] 1.1 Animal grouping

[0247] 93 8-week-old Beagle dogs were randomly divided into 31 groups, all of which were negative for H3 subtype influenza virus antibody in HI test: Group 1-1, Group 1-2, Group 1-3, Group 1-4, Group 1- Groups 5 and 1-6 are H3N2 (A / canine / Beijing / 0528-147 / 2017 147) and H3N8 canine influenza virus whole virus bivalent inactivated vaccine groups; Group 2-1, Group 2-2, Group 2- Group 3, Group 2-4, Group 2-5 and Group 2-6 are H3N2 (A / canine / Quanzhou / 1224-1109 / 2018) and H3N8 canine influenza virus whole virus bivalent inactivated vaccine group; Group 3-1 , Group 3-2, Group 3-3, Group 3-4 Group 3-5 and Group 3-6 are bivalent recombinant virus inactivated vaccine groups for H3N2-2017 and H3N8 canine influenza virus; Group 4-1, 4 -2 groups, 4-3 groups, 4-4 groups...

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Abstract

The invention discloses an H3N2 and H3N8 subgenetype dog influenza divalent inactivated vaccine and a preparation method and application thereof, and provides a dog influenza virus vaccine. The vaccine comprises a vaccine antigen which is totivirus bivalent inactivated vaccine antigen consisting of (1) or (2): (1) A / canine / Quanzhou / 1224-1109 / 2018(H3N2)CGMCCNO.18180 and an H3N8 subgenetype dog influenza virus influenza virus strain; and (2)a recombinant virus bivalent inactivated vaccine antigen consisting of a recombinant H3N2 influenza virus obtained by A / canine / Quanzhou / 1224-1109 / 2018(H3N2)CGMCC and a recombinant H3N8 subgenetype influenza virus strain obtained by the H3N8 subgenetype influenza virus strain. The H3N2 and H3N8 subgenetype dog influenza totivirus bivalent inactivated vaccine and the recombinant virus(H3N2 / PR8+H3N8 / PR8) bivalent inactivated vaccine both have favorable protective effects on different branch H3N2 subgenetype dog influenza virus and H3N8 subgenetype dog influenza virus.

Description

technical field [0001] The invention belongs to the field of vaccine production, and relates to the preparation of H3N2 and H3N8 subtype canine influenza whole virus bivalent inactivated vaccines and recombinant virus (H3N2 / PR8+H3N8 / PR8) bivalent inactivated vaccines, especially H3N2 and H3N8 subtype dogs Bivalent inactivated influenza vaccine and its preparation method and application. Background technique [0002] Vaccination is currently the main means of preventing and controlling animal diseases in my country. The use of inactivated vaccines can be traced back to 1945. In 1945, whole-virus inactivated vaccines began to be used in the United States and clinical efficacy observations were carried out. Because it retains all the antigenic components of the influenza virus, it is better in terms of immunogenicity and safety, there will be no variation and virulence recovery, and the preparation process is simple, and the finished product is easy to store. It is widely used...

Claims

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Application Information

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IPC IPC(8): A61K39/145A61K39/295A61P31/16
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/70A61P31/16C12N2760/16134
Inventor 孙怡朋刘金华陈明月
Owner CHINA AGRI UNIV
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