Cellulosimicrobium cellulans DGNK-JJ1 and application thereof

A DGNK-JJ1, fiber microbacteria technology, applied in the field of agricultural biology, can solve the problems of agricultural product quality and safety, pesticide residues, environmental pollution, etc., to avoid or reduce ecological environment problems, develop ecological agriculture, and avoid or reduce agricultural product quality and safety. effect of the problem

Active Publication Date: 2020-01-14
DONGGUAN AGRI SCI RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to increase crop production and income, a large amount of potassium fertilizer is used in agricultural production for a long time, which is likely to cause a series of ecological environmental problems and agricultural product quality and safety problems such as soil compaction, environmental pollution, and pesticide residues.

Method used

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  • Cellulosimicrobium cellulans DGNK-JJ1 and application thereof
  • Cellulosimicrobium cellulans DGNK-JJ1 and application thereof
  • Cellulosimicrobium cellulans DGNK-JJ1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The separation and screening of embodiment 1 potassium decomposing bacteria

[0026] Under sterile conditions, weigh 10g of soil sample, dissolve it in 90mL of sterile water, fully shake and gradiently dilute to 10 －3 、10 －4 、10 －5 times of compost suspension, and then 100 μL of each dilution was evenly spread on the potassium-dissolving bacteria medium plate, and cultured upside down at 37°C for 3 days. Select the largest colony that is colorless, transparent, and oil-drop-shaped, and perform purification by streaking on the plate, and repeat the process 3 times. At the same time, observe the purity of the colony with a microscope until a pure culture is obtained. Finally, the obtained pure culture strain was transferred into glycerol with a final concentration of 30% and sealed and stored in a -20°C refrigerator.

Embodiment 2

[0027] The mensuration of the potassium decomposing ability of embodiment 2 potassium decomposing bacteria

[0028] The primary screened strain obtained in Example 1 was inoculated into 30 mL of LB medium, and cultured at 36° C. at 150 r / min for 1 d as a seed solution. The seed solution was absorbed according to the inoculum amount of 2% and added to 100mL potassium-dissolving bacteria culture medium. The culture medium without bacteria was used as the blank control group. Each treatment was set up with 3 replicates, and cultured at 36°C and 150r / min for 7 days. Samples were taken on 3d, 5d, and 7d respectively. The fermentation broth was centrifuged at 4°C and 6000r / min for 10 minutes, and the supernatant was taken. Using potassium chloride as a standard substance, the content of available potassium in the solution was measured with an atomic absorption spectrophotometer. Then calculate the relative increase rate of available potassium (the results are shown in Table 1):

[...

Embodiment 3

[0034] The identification of embodiment 3 potassium decomposing bacteria

[0035] (1) Morphological observation. According to "Bergey's Bacteria Identification Handbook", observe the morphology, color, etc. of the surface growth colonies of the potassium-dissolving bacteria culture medium obtained in Example 1; pick young cultures, smear, Gram staining, observe the thalline morphology, Size, Gram staining reaction, and the presence or absence, shape and location of spores, etc., the results are shown in Table 2, figure 1 and figure 2 .

[0036] Table 2 Colony culture characteristics and microscopic examination results

[0037]

[0038] (2) Physiological and biochemical tests. Nitrate reduction test, sugar fermentation test, escin hydrolysis test, β-galactosidase test, β-glucuronidase, gelatin hydrolysis test, urease test, alkaline phosphatase test and pyrrolidone Enzyme experiment (PYR experiment), etc.

[0039] The potassium-solubilizing bacterial strain of the pres...

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Abstract

The invention discloses cellulosimicrobium cellulans DGNK-JJ1 collected in Guangdong microbial collection center (GDMCC) on August 22, 2019 in No.59 building 5 floor of No.100 yard in Xianlie Middle Road in Guangzhou city in Guangdong province with postcode of 510075 and collection number of GDMCC NO:60749. Bacterium DGNK-JJ1 capable of efficiently decomposing potassium is separated and screened from soil samples of soil after composting of traditional Chinese medicine residues and is identified, an effective strain is provided for production of potassium decomposing bacterium fertilizer, theecological environment problem and agricultural product quality safety problem can be solved or relieved, and development of ecological agriculture is promoted.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a cellulosimicrobium cellulans DGNK-JJ1 and its application. Background technique [0002] Potassium is an essential nutrient element for plant growth and development, and the supply of potassium in the soil directly affects the yield and quality of crops. Potassium content in the soil is rich, but mostly exists in the form of insoluble minerals that are extremely difficult for plants to absorb. The available potassium that can be directly absorbed and utilized by crops is generally between 40mg / kg-200mg / kg, accounting for only 1%-5 of the total potassium. %, cannot meet the needs of crops. The forms of phosphorus and potassium in soil in my country are mainly insoluble phosphorus and mineral potassium that are not easily absorbed and utilized by crops, and the resources of phosphorus and potassium that can be directly absorbed and utilized by plants are extremely limit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/02C12R2001/01C12N1/205Y02W30/40
Inventor 梁卫驱胡珊黄皓陈仕丽罗华建徐匆
Owner DONGGUAN AGRI SCI RES CENT
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