A strain of Penicillium mj51 and its application
A technology of MJ51 and Penicillium, applied in the field of microbial agent
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Embodiment 1
[0068] 1) Ingredients
[0069] Inoculate Penicillium MJ51 (CGMCC No.18120) on PDA solid medium, inoculate vigorously growing Penicillium MJ51 mycelium pieces (0.5*0.5cm) into PDA liquid medium, place on a constant temperature shaker at 28°C, Cultivate at 150rpm for 3 days until a large number of mycelium balls appear in the medium, and after mixing evenly, the culture is obtained;
[0070] 2) fermentation
[0071] Under sterile conditions, 10 mL of the culture was added to 100 ml of lignite liquid medium, shaken up immediately, placed in a shaker at 28° C., with a rotation speed of 150 rpm, and the culture time was 7 days.
[0072] The lignite liquid culture medium contains water as a solvent: a mass concentration of 0.5% (NH 4 ) 2 SO 4 , with a mass concentration of 0.2% K 2 HPO 4 , KH with a mass concentration of 0.1% 2 PO 4 , MgSO with a mass concentration of 0.03% 4 ·7H 2 O, CaCl with a mass concentration of 0.002% 2 2H 2 O and mass concentration are 1% lignite...
Embodiment 2
[0084] 1) Ingredients
[0085] Based on the mass percentage of the total amount of ingredients, lignite 45%, corn stalk 20%, (NH 4 ) 2 SO 4 2% and water 33%.
[0086] 2) Operation steps
[0087] Mix and stir all kinds of materials evenly, add appropriate amount of water (based on the principle of wet and loose materials, (NH 4 ) 2 SO 4 Dissolve in water and add), stir evenly after standing for 30 minutes, insert the culture with 10% inoculum (Penicillium MJ51 (CGMCC No.18120) is inoculated on the PDA solid medium, and the Penicillium MJ51 bacterium that grows vigorously Silk pieces (0.5*0.5cm) were inoculated into PDA liquid medium, placed in a constant temperature shaker at 28°C, 150rpm and cultivated for 3 days, until a large number of mycelium balls appeared in the medium, after mixing evenly, the culture was obtained), at 28°C~ Culture at 30°C for 7-10 days. After the fermentation, the method described in Example 1 was used to extract fulvic acid at 25°C.
[0088]...
Embodiment 3
[0098] 1) Strain culture
[0099] Inoculate the Penicillium MJ51 mycelium block (0.5*0.5cm) that grows vigorously on the PDA solid medium into the PDA solid medium, and place it in a constant temperature incubator at 28°C for 3 to 5 days until the plate is completely covered by colonies.
[0100] 2) Plate liquefaction test
[0101] After the lignite is sterilized, it is evenly sprinkled on the Penicillium MJ51 that has been cultivated on the PDA plate at 28°C for three days under aseptic conditions. droplet formation. At the same time, the same amount of coal was sprinkled on a sterile PDA plate as control B, and Penicillium MJ51 grown for the same number of days was used as control A. The two control groups were placed in a constant temperature incubator together with the treatment group for cultivation.
[0102] 3) Product analysis
[0103] After cultivating for three days, small black droplets appeared on the MJ51+ coal plate of the treatment group, while there was no s...
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