Method for analyzing phylogenetic development of babylonia formosae based on complete mitochondrial genomes

Abstract

The invention discloses a method for analyzing phylogenetic development of babylonia formosae based on complete mitochondrial genomes, and relates to the field of analysis of the phylogenetic development of the babylonia formosae. The method for analyzing phylogenetic development of the babylonia formosae based on the complete mitochondrial genomes comprises the following steps: 1) sample collection: collecting babylonia formosae extracting mitochondrial genomes DNA; 2), disrupting and purifying the mitochondrial genomes DNA and then building a DNA sequencing library; 3), obtaining a DNA cluster; 4), sequencing the babylonia formosae mitochondrial genomes DNA; 5), comparing the babylonia formosae mitochondrial genomes with other babylonias having known sequences, and then noting encoded protein genes; and 6), comparing a nucleotide sequence of each gene of the babylonia formosae extracting mitochondrial genomes DNA and an amino acid sequence deduced by the nucleotide sequence with other caenogastropoda species, and performing series connection and then analyzing the phylogenetic development. According to the phylogenetic analysis method of the babylonia formosae based on the complete mitochondrial genomes disclosed by the invention, based on the complete mitochondrial genomes, the phylogenetic development of the babylonia formosae is analyzed, complete data of the complete mitochondrial genomes of the babylonia formosae are obtained, and thus, phylogenetic development is performed, and analysis is performed conveniently and effectively.

Description

technical field [0001] The invention relates to the field of phylogenetic analysis of Taiwan Dongfeng snails, in particular to a method for analyzing the phylogeny of Taiwan Dongfeng snails based on the whole mitochondrial genome. Background technique [0002] Taiwan Dongfeng snail (Babylonia formosae) belongs to the mollusc phylum, Gastropoda, Mollidae, Dongfeng snail genus. The taxonomy and phylogenetic relationship of Mollidae mainly depend on the morphological characteristics of shells and shells so far. However, because the engraving, color and shape of the shell surface are easily affected by environmental factors, there is greater instability, coupled with the convergent evolution of similar species, the shells of the Mollidae species tend to be simplified, so they are not Do not provide enough distinguishing features, so classification based solely on the shell and shell morphology often leads to errors and confusion in classification. The phylogenetic relationship...

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the phylogenetic relationship between the classification and the phylogenetic relationship of the Taiwan Dongfeng snail system of the Mollidae species by using morphological methods, because the engraving, color and shape of the shell surface are easily affected by environmental factors, and there is a relatively large problem. Instability, coupled with the convergent evolution of similar species, makes the shells of the Mollidae species tend to be simplistic, and thus cannot provide enough distinguishing features, resulting in classifications that rely solely on the morphology of the shells and shells often lead to classification In order to solve the problems of errors and confusion in the environment, a method for analyzing the phylogeny of Dongfeng snails in Taiwan based on the whole mitochondrial genome was proposed

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