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A kind of probiotic composition and its preparation and application

A technology of probiotics and compositions, applied in the field of probiotic preparations, can solve problems such as unclear mechanisms, and achieve the effect of alleviating RSV virus infection

Active Publication Date: 2021-06-25
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic scholars have found that probiotics have a good effect in the treatment of infantile bronchopneumonia, but the mechanism is not clear

Method used

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  • A kind of probiotic composition and its preparation and application
  • A kind of probiotic composition and its preparation and application
  • A kind of probiotic composition and its preparation and application

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0045] The present invention can be better understood through the following experimental procedures.

[0046] (1) The experimental process is as follows:

[0047] (1) RSV virus amplification Use RPMI1640 containing 10% fetal bovine serum as the RSV maintenance solution, and the human laryngeal carcinoma cells (Hep-2) passed to the second generation are RSV reproductive cells, and the Hep-2 cells grow and fuse until dense Virus amplification experiments were performed after the monolayer. Discard the cell culture medium before amplification, wash with PBS buffer three times, inoculate 5 mL of RSV-A type A2 standard strain, 37°C, 5% CO 2 Adsorption under constant temperature conditions for 2h. After the adsorption was completed, the virus liquid was discarded, and 30 mL of RPMI1640 maintenance solution with 10% fetal bovine serum was added for cultivation. Observe day by day, when more than 80% of the cells have lesions, the cells are ultrasonically disrupted, centrifuged at ...

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Abstract

The invention discloses a probiotic composition and its preparation and application, comprising VSL#3, Lactobacillus rhamnosus M9 and Lactobacillus plantarum P‑8; the VSL#3, rhamnosus Saccharobacillus M9 and Lactobacillus plantarum P‑8 were mixed in an equal ratio of 1:1:1. The application of the probiotic composition in the preparation of medicines for treating or preventing pneumonia, especially pneumonia caused by respiratory syncytial virus infection, especially infant bronchopneumonia caused by respiratory syncytial virus infection. The probiotic composition of the present invention can alleviate the change of the inflammatory pathological structure of the lung tissue caused by RSV infection, and can inhibit the virus infection process of RSV infection; it can activate TLR3 and RIG-1 signaling pathways in alveolar macrophages to promote IFN-1 The production of β can inhibit the expression of lung inflammatory factors; it can regulate the lung flora and intestinal flora to relieve RSV virus infection.

Description

technical field [0001] The invention belongs to the field of probiotic preparations, and in particular relates to a probiotic composition and its preparation and application. Background technique [0002] Probiotics are a class of active microorganisms that can promote the balance of intestinal microbial flora and have beneficial effects on the health or physiological functions of the host. At present, it is widely used in the fields of life and health, scientific research, bioengineering, industry and agriculture, and food safety. A large number of domestic and foreign studies have shown that probiotics have positive effects in lowering blood pressure, lowering blood sugar, lowering blood fat, anti-allergy, anti-inflammation, regulating immunity, maintaining the balance of intestinal flora, etc., and have very broad application prospects in functional foods and medicines . Probiotics can play an immunomodulatory role in various ways. For example, probiotics can directly e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A23L33/135A61P11/00A61P31/14A61K35/741
CPCA61K35/747A61K35/741A61P31/14A61P11/00A23L33/135A23V2002/00A61K2300/00A23V2200/314A23V2400/175A23V2400/169
Inventor 吕志刚纪建建孙沁梅王倩
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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