Bone regeneration guiding collagen membrane for dental use and preparation method thereof

A technology for guiding regeneration and collagen membrane, applied in the fields of medical science, pharmaceutical formulation, surgery, etc., it can solve the problems of inability to effectively prevent soft tissue from growing into the tooth socket, unfavorable exchange of materials in trauma, occupying space for bone tissue growth, etc. Membrane degradation time, beneficial to repair and healing, and the effect of controllable degradation

Active Publication Date: 2020-02-04
BEIJING YH BIOMAX BIOLOGIC TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, due to the addition of chondroitin sulfate in the prepared oral biofilm, and chondroitin sulfate has a moderate anticoagulant effect, it is unfavorable for trauma hemostasis in oral restorative surgery; secondly, the prepared oral biofilm is a porous structure. After surgery, the soft tissue cannot be effectively prevented from growing into the tooth socket, occupying the growth space of bone tissue, and affecting the growth of bone tissue; in addition, the prepared oral biofilm is thicker, 1-3mm, which is not conducive to the material in the wound site later. Exchange, which is not conducive to postoperative repair

Method used

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  • Bone regeneration guiding collagen membrane for dental use and preparation method thereof
  • Bone regeneration guiding collagen membrane for dental use and preparation method thereof
  • Bone regeneration guiding collagen membrane for dental use and preparation method thereof

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preparation example

[0052] Extraction and purification of type I collagen:

[0053] Type I collagen is generally extracted, separated, and purified as follows: First, the biological tissue is broken, and the protein is extracted with an appropriate buffer, and then the cell fragments are separated from the solution by centrifugation, and then salted out or organic solvent. The relevant protein tissue is precipitated, and then the various proteins are separated by chromatography or electrophoresis, and finally the protein is crystallized or made into a freeze-dried powder under appropriate conditions (specific reference: Jiang Tingda, Collagen and Collagen [M] ], P 90).

[0054] The extraction and purification method for extracting type I collagen from bovine Achilles tendon is a conventional technical means, and type I collagen can be extracted from bovine Achilles tendon by the following method.

preparation example 1

[0056] Type I Collagen Extracted from Bovine Achilles Tendon

[0057] After washing the fresh bovine Achilles tendon, remove fat, aponeurosis and muscle, cut into pieces (the size of the piece can be 0.5cm×0.5cm), add a mass fraction of 10% sodium bicarbonate aqueous solution and soak for 16h (sodium bicarbonate aqueous solution It’s fine if the beef hasn’t passed through), take it out, wash it with deionized water, and dry it to get pure beef Achilles tendon;

[0058] Pure bovine Achilles tendon is added to the myristic acid solution containing 5% mass fraction of triethanolamine and soaked for 2-3h, taken out, cleaned with deionized water, and dried; the content of triethanolamine contained in every 1L of myristic acid solution is 0.15g;

[0059] Add the above-mentioned dried beef Achilles tendon into liquid nitrogen, and grind the beef Achilles tendon into powder after it stops boiling to obtain beef Achilles tendon powder;

[0060] Get 20g bovine Achilles tendon powder, ...

preparation example 2

[0071] Type I Collagen Extracted from Bovine Achilles Tendon

[0072] Remove the meat and fat from 80 g of beef Achilles tendon, grind it with a meat grinder, wash it with water and 1% to 10% (wt, %) sodium chloride solution in decibels three times, and then wash it with water until the effluent is not cloudy. Suspend the clean bovine Achilles tendon in dilute acetic acid and stir slowly for 24-72 hours below 25°C. After filtering, add 250-1000g sodium chloride to the filtrate, and the collagen is precipitated in the form of milky white fiber product. Then filter, wash with distilled water, resuspend the filter cake in dilute acetic acid and extract 3-4 times according to the above method. The last filter cake was suspended in 5L of 0.5mol / L acetic acid, and stirred until completely gelled, about 24h. Add 500g of sodium chloride for salting out, and then wash the precipitate with distilled water, then place the precipitate in 0.5L of 0.5mol / L acetic acid, stir until the crys...

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Abstract

The invention discloses bone regeneration guiding collagen membrane for dental use and a preparation method thereof, and belongs to the technical field of regenerative medical materials. The preparation method of the bone regeneration guiding collagen membrane for dental use comprises the following steps: S1, preparing a raw material and carrying out pretreatment, including: S1-1, dissolving type-I collagen in an acidic solvent and carrying out homogenization so as to obtain collagen slurry, S1-2, filtering the collagen slurry, and S1-3, performing negative-pressure pumping on the collagen slurry filtered in S1-2 until no obvious bubbles exist; S2, carrying out freeze-drying, namely freeze-drying the collagen slurry prepared in S1 so as to obtain collagen sponge; S3, pressing the collagensponge into a thin piece, namely pressing the collagen sponge into a thin piece under a pressure of 1-5 MPa; and S4, performing severe dehydration and cross-linking on the thin piece in vacuum so as to obtain the bone regeneration guiding collagen membrane for dental use, and carrying out sterilizing and storing. The bone regeneration guiding collagen membrane for dental use disclosed by the invention can be used to stop bleeding and guide bone regeneration after dental implantation, and has the advantages of being low in immunogenicity, excellent in biocompatibility and capable of effectivelypromoting bone regeneration. Moreover, the preparation method of the bone regeneration guiding collagen membrane for dental use has the advantages of being efficient and easy in operation, non-toxicand harmless.

Description

technical field [0001] The invention relates to the technical field of regenerative medical materials, more specifically, it relates to a bone-guiding regenerative collagen film in the department of stomatology and a preparation method thereof. Background technique [0002] In recent years, with the continuous development of implant technology, dental implant technology has played an increasingly important role in the restoration of missing teeth and achieved good results. However, in actual clinical practice, due to factors such as bone tissue defect and bone resorption, the alveolar ridge is too low or partially depressed in some patients, resulting in lateral perforation, which leads to failure of implant placement. [0003] With the application of guided bone regeneration (Guided Bone Regeneration, GBR), the above problems have been solved to a certain extent. The specific clinical operation of dental implant technology is to form a tooth socket after tooth extraction, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/14A61L31/16
CPCA61L31/044A61L31/14A61L31/16A61L2400/04A61L2300/252
Inventor 崔蓉蓉张艳勤刘恒
Owner BEIJING YH BIOMAX BIOLOGIC TECH
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