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Method for identifying whether non-milk powder type exogenous protein is adulterated in honey or not

A protein and honey technology, applied in the field of food testing, analysis and testing, can solve the problem of difficult detection of adulterated honey, and achieve the effect of being suitable for promotion, improving competitiveness and low detection limit

Inactive Publication Date: 2020-02-04
ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, another type of adulterated honey has appeared on the market. These honeys meet the requirements of stable carbon isotope ratios in GB / T 18932.1-2002, but other spectroscopic methods clearly show that they are mixed with syrupy substances.
[0008] The discrepancy between the test results shows that the means of counterfeiting have been upgraded. Unscrupulous businessmen evade the existing detection means by adding non-milk powder exogenous proteins, making it difficult to detect adulterated honey

Method used

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  • Method for identifying whether non-milk powder type exogenous protein is adulterated in honey or not
  • Method for identifying whether non-milk powder type exogenous protein is adulterated in honey or not
  • Method for identifying whether non-milk powder type exogenous protein is adulterated in honey or not

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Determination of characteristic detection peaks of non-milk powder class exogenous proteins

[0023] First, collect pure honey from different regions and different types. There are 161 pure honeys, including: acacia honey, rapeseed honey, vitex honey, linden honey, jujube honey, sunflower honey, buckwheat honey, vetch honey, cotton honey, lychee honey, longan honey, wolfberry honey and potpourri honey, The origin of honey includes: Xinjiang, Sichuan, Inner Mongolia, Hubei, Liaoning, Jiangsu, Henan, Hebei, Jilin, Shaanxi, Shandong, Gansu and other provinces.

[0024] These samples can cover all types of honey that are common today.

[0025] The above-mentioned samples are respectively marked as pure honey sample 1, pure honey sample 2, pure honey sample 3, ... pure honey sample n.

[0026] Adulterated honey spiked with exogenous proteins other than milk powder is then collected. These honeys have the following two characteristics: first, these honey samples ...

Embodiment 2

[0039] Preparation of Characteristic Substance Compound A

[0040] Compound A, as a characteristic substance of non-milk powder adulterated protein, has a relatively high content. Take 100g of a non-milk powder adulterated protein and pre-treat it as a honey sample. 7.8 ± 0.2min of the compound (hereinafter referred to as compound A), a total of 1.6g after drying. That is, the content of characteristic compound A in non-milk powder adulterated protein is 16g / kg.

[0041] After the characteristic substance Compound A was prepared, it was tested for stability test for 6 months, and it was found that the substance existed stably in non-milk powder adulterated protein during 6 months.

Embodiment 3

[0043] Determination of detection method for characteristic substance compound A of non-milk powder exogenous protein

[0044] 1. Pretreatment

[0045]Weigh about 2.0g of sample into a 50mL centrifuge tube, add 1mL of 0.1mol / L phosphoric acid solution and 4mL of ethanol, vortex and dissolve in a water bath at 60°C for 10min; then transfer to a 10mL volumetric flask, add mobile phase (acetonitrile: 0.1% phosphoric acid aqueous solution = 30:70 (volume ratio)) and set the volume to the mark; filter through 0.45 μm organic phase and aqueous phase filter membranes into the sample bottle for testing.

[0046] 2. Instrument Analysis Conditions

[0047] Chromatographic conditions: chromatographic column is Kinetex C18 column (100mm×4.6mm, 2.6μm)

[0048] Column temperature: 40°C

[0049] Injection volume 5μL

[0050] Mobile phase: A is acetonitrile, mobile phase B is 0.1% phosphoric acid aqueous solution, isocratic elution, mobile phase ratio A:B=30:70

[0051] Flow rate: 0.2mL·...

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Abstract

The invention relates to the field of analysis and detection, in particular to the field of food detection, and more particularly relates to a method for identifying adulterated honey. The method comprises the following steps of observing whether an absorption peak is existent at the retention time of 7.8+ / -0.2min under the ultraviolet detection wavelength of 210nm based on the liquid-phase chromatographic analysis method, and if the absorption peak is detected, judging that the non-milk powder type exogenous protein is adulterated in a honey sample. Through the method disclosed by the inventio, the malicious counterfeiting behaviors of illegal vendors can be effectively identified, and the method is a reliable and effective method for identifying adulteration of the non-milk powder type exogenous protein.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to the field of food detection, more specifically to a method for identifying adulterated honey. Background technique [0002] Honey is a natural nutrient with unique health benefits. With the improvement of the quality of life of the people, the demand for this natural health product is increasing, and the honey industry has gradually become an indispensable part of economic development and foreign trade. [0003] In recent years, my country's honey industry has developed rapidly, and its output ranks first in the world. However, a small number of unscrupulous businessmen are mercenary, mixing relatively cheap syrup and protein into expensive pure honey. The incidents of honey adulteration and counterfeiting have occurred from time to time, and this kind of honey adulteration has greatly impacted the bee product market. Not only did it deceive consumers, but it also had a s...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 季美泉吴斌王艳丁涛费晓庆张健郭思言李静静姜珊刘芸陈磊刘艳张晓燕柳菡沈伟健高玲王茂华唐茂芝吴海文
Owner ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU
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