Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of specific antibody, implantable medical instrument and preparation method of implantable medical instrument

An implanted medical device, specific technology, applied in the field of biomedicine, can solve the problems of vascular restenosis, large side effects of drugs, poor specificity, etc., and achieve the effect of promoting endothelial repair, small side effects of drugs, and ideal comprehensive curative effect

Inactive Publication Date: 2020-02-07
SHANGHAI MICROPORT MEDICAL (GROUP) CO LTD
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, medical devices that promote endothelial repair are generally realized by loading small molecular compounds or compositions on the surface of medical devices, all of which are based on the mechanism of promoting cell growth, but generally have poor specificity. Such as the growth of other cells, resulting in adverse consequences such as vascular restenosis, thrombosis, etc., this way of drug side effects, will bring other adverse effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of specific antibody, implantable medical instrument and preparation method of implantable medical instrument
  • Application of specific antibody, implantable medical instrument and preparation method of implantable medical instrument
  • Application of specific antibody, implantable medical instrument and preparation method of implantable medical instrument

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 100 μg of AC133 monoclonal antibody Clone AC133 (purchased from Miltenyi Biotechnology Co., Ltd., Germany) was dissolved in 10 mL of antibody diluent to prepare an antibody solution with a concentration of 10 μg / mL. Polyelectrolyte sodium hyaluronate (HA) 1mg / mL dissolved in 0.1% NaCl solution, polyelectrolyte chitosan (CS) 1mg / mL dissolved in 0.1% NaCl solution, base coat polyelectrolyte polyimide (PEI) 5mg / mL dissolved in 0.1% NaCl solution.

[0030] The stent was treated with H 2 SO 4 :H 2 o 2 Soak in (30%, v / v)=3:1 solution for 30 minutes, rinse with purified water for 3 times, and blow dry with nitrogen. First immerse in polyethylimide (PEI) solution to obtain PEI layer; immerse PEI-treated scaffold in sodium hyaluronate (HA) solution to obtain HA outer layer; then immerse scaffold in chitosan (CS) solution Get the outer layer of CS. The above HA / CS electrostatic assembly process was repeated 7 times to obtain a sodium hyaluronate / chitosan self-assembled mult...

Embodiment 2

[0032] 100 μg of AC133 monoclonal antibody Clone AC133 (purchased from Miltenyi Biotechnology Co., Ltd., Germany) was dissolved in 1 mL of antibody diluent to prepare an antibody solution with a concentration of 100 μg / mL. The stent was treated with H 2 SO 4 :H 2 o 2 = Soak in 3:1 solution for 30 minutes, rinse with purified water for 3 times, and blow dry with nitrogen. Immerse in the antibody solution, let it stand for 30 minutes, take it out, dry it naturally, and store it at 4°C. Complete the preparation of AC133 antibody-loaded scaffolds. After weighing and calculating, the concentration of AC133 antibody per unit area on the surface of the scaffold was finally measured to be 71 μg / mm 2 .

Embodiment 3

[0034] 1 mg of AC133 monoclonal antibody Clone AC133 (purchased from Miltenyi Biotechnology Co., Ltd., Germany) was dissolved in 1 mL of antibody diluent to prepare an antibody solution with a concentration of 1 mg / mL. The antibody solution was sprayed onto the surface of the left atrial appendage occluder membrane with an ultrasonic sprayer, dried naturally, and stored at 4°C. Complete the preparation of AC133 antibody-loaded scaffolds. After weighing calculation, the concentration of AC133 antibody per unit area of ​​the membrane surface was finally measured to be 690 μg / mm 2 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to application of a specific antibody, an implantable medical instrument and a preparation method. The application is the application of the specific antibody of a hematopoieticstem cell marker in preparing a medicine for promoting endothelial repair. The specific antibody of the hematopoietic stem cell marker is loaded on the surface of the implantable medical instrument. Apreparation method comprises: a step of loading the specific antibody of the hematopoietic stem cell marker on at least a part of the surface of the implantable medical instrument, and a step of loading the specific antibody of the hematopoietic stem cell marker in a hole or a groove of the implantable medical instrument. According to the technical scheme provided by the invention, the specific antibody of the hematopoietic stem cell marker is creatively used for realizing the effect of promoting endothelial repair of the medical instrument. Compared with an existing endothelial repair mode,the specific antibody captures hematopoietic stem cells of a human body and induces to generate cells to achieve targeted endothelial repair. The mode has small medicine side effect, no other adverseeffects and more ideal comprehensive curative effect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the use of a specific antibody, an implanted medical device and a preparation method thereof. Background technique [0002] In 1997, Yin et al confirmed that AC133 is a marker of hematopoietic stem cells. In 2000, AC133 was officially named CD133 at the 7th International Working Conference on Human Leukocyte Differentiation Antigen held in Harrogate, UK. The CD133 antigen was recognized by three CD133 antibodies: clones AC133, 293C3 and AC141. Clone AC133 directly binds to CD133 / 1 glycosylation epitope, and can be used to analyze and sort CD133 positive cells from peripheral blood, bone marrow, umbilical cord blood and other tissues. Monoclonal antibodies 293C3 and AC141 recognize the epitope CD133 / 2 and are mainly used for fluorescent staining of CD133+ cells after MACS sorting. [0003] The left atrial appendage is an ear-shaped sac protruding from the left at...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61P9/14A61L27/50A61L27/54A61L27/34
CPCA61K2039/505A61L27/34A61L27/507A61L27/54A61L2300/412A61L2430/14A61L2430/20A61L2430/22A61P9/14C07K16/2896C08L89/00A61F2/00A61F2/06A61K39/395A61L27/50A61L31/08
Inventor 王玄周婷李俊菲姚瑶
Owner SHANGHAI MICROPORT MEDICAL (GROUP) CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com