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Purpose of specific antibody, implanting medical apparatus and preparation method of implanting medical apparatus

An implanted medical device and specific technology, which is applied in the direction of antibody medical components, medical preparations containing active ingredients, antibodies, etc., can solve the problems of poor specificity, restenosis of blood vessels, and large side effects of drugs, so as to achieve small drug side effects and promote Ideal effect of endothelial repair and comprehensive curative effect

Inactive Publication Date: 2020-02-07
SHANGHAI MICROPORT MEDICAL (GROUP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, medical devices that promote endothelial repair are generally realized by loading small molecule compounds or compositions on the surface of medical devices, all of which are based on the mechanism of promoting cell growth, but generally have poor specificity. Such as the growth of other cells, resulting in adverse consequences such as vascular restenosis, thrombosis, etc., this way of drug side effects, will bring other adverse effects

Method used

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  • Purpose of specific antibody, implanting medical apparatus and preparation method of implanting medical apparatus
  • Purpose of specific antibody, implanting medical apparatus and preparation method of implanting medical apparatus
  • Purpose of specific antibody, implanting medical apparatus and preparation method of implanting medical apparatus

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Experimental program
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Effect test

Embodiment 1

[0035] 100 g of Bcrp1 monoclonal antibody Clone 5D3 (purchased from Miltenyi Biotechnology Co., Ltd., Germany) was dissolved in 10 mL of antibody diluent (Wuhan Boster) to prepare an antibody solution with a concentration of 10 g / mL. Polyelectrolyte sodium hyaluronate (HA) 1mg / mL dissolved in 0.1% NaCl solution, polyelectrolyte chitosan (CS) 1mg / mL dissolved in 0.1% NaCl solution, base coat polyelectrolyte polyimide (PEI)5

[0036] mg / mL dissolved in 0.1% NaCl solution.

[0037]Wash bare brackets and blow dry. First, soak in polyacetylimide (PEI) solution to obtain PEI layer; soak the PEI-treated scaffold in sodium hyaluronate (HA) solution to obtain the outer layer of HA; then dip the scaffold in chitosan (CS ) solution, the CS outer layer was obtained. The above HA / CS electrostatic assembly process was repeated 7 times to obtain a sodium hyaluronate / chitosan self-assembled multilayer film, which was denoted as PEI(HA / CS)7. Soak the scaffold in the antibody solution for 3...

Embodiment 2

[0039] 100 g of MDR1 monoclonal antibody Clone REA495 (purchased from Miltenyi Biotechnology Co., Ltd., Germany) was dissolved in 1 mL of antibody diluent (Wuhan Boster) to prepare an antibody solution with a concentration of 100 g / mL. Laser cut L605 bare stents with H 2 SO 4 :H 2 o 2 = Soak in 3:1 solution for 30 minutes, rinse with purified water for 3 times, and blow dry with nitrogen. Immerse in the antibody solution, let it stand for 30 minutes, take it out, dry it naturally, and store it at 4°C. Complete the preparation of the Clone REA495 load stent. After weighing calculation, the concentration of Clone REA495 per unit area on the surface of the stent was finally measured to be 89g / mm 2 .

Embodiment 3

[0041] 1 mg of MDR1 monoclonal antibody Clone REA495 (purchased from Miltenyi Biotechnology Co., Ltd., Germany) was dissolved in 1 mL of antibody diluent (Wuhan Boster) to prepare an antibody solution with a concentration of 1 mg / mL. The antibody solution was sprayed onto the surface of the left atrial appendage occluder membrane with an ultrasonic sprayer, dried naturally, and stored at 4°C. Complete the preparation of Clone REA495 loaded membrane. After weighing and calculating, the concentration of Clone REA495 per unit area of ​​the membrane surface was finally measured to be 780g / mm 2 .

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Abstract

The invention belongs to a purpose of a specific antibody, an implanting medical apparatus and a preparation method of the implanting medical apparatus. The specific antibody of an ATP combined box protein is applied to preparation of medicines for promoting endothelium repair. The surface of the implanting medical apparatus is loaded with the specific antibody of the ATP combined box protein. Thepreparation method comprises a working procedure of loading the specific antibody of the ATP combined box protein to at least a part of the surface of the implanting medical apparatus and a working procedure of loading the specificity of the ATP combined box protein to a hole or a groove of the implanting medical apparatus. The technical scheme provided by the invention creatively uses the specific antibody of the ATP combined box protein to realize the efficacy of the medical apparatus for promoting endothelium repair, compared with a conventional endothelium repair manner, a manner of usingthe implanting medical apparatus disclosed by the invention has the advantages that the implanting medical apparatus can capture autologous hematopoietic stem cells of a human body and can induce togenerate cells to realize pointed endothelium repair, and the manner is small in medicine side effects, cannot cause other adverse effects and is ideal in comprehensive treatment effects.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the use of a specific antibody, an implanted medical device and a preparation method thereof. Background technique [0002] Atherosclerotic disease can lead to damage to the vascular endothelium, resulting in thrombosis, inflammation and other diseases, leading to myocardial infarction. If the damaged tissue cannot be repaired during treatment, it will lead to intimal hyperplasia, restenosis of blood vessels, and endanger the life of the patient. Hematopoietic stem cells are adult stem cells in the blood system, which have long-term self-renewal ability and the potential to differentiate into various mature blood cells. Substances that can capture hematopoietic stem cells on the surface of medical devices implanted in the blood system can be induced to differentiate in situ into the required cell types according to requirements, which can improve the therapeutic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61L27/34A61L31/10A61P43/00
CPCA61K39/3955A61K2039/505A61L27/34A61L31/10A61L2420/02A61P43/00A61F2/00A61F2/06A61K39/395A61L31/08
Inventor 王玄陈陆李俊菲
Owner SHANGHAI MICROPORT MEDICAL (GROUP) CO LTD
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