Method for separating and purifying jujube polysaccharide from plants

A jujube polysaccharide, separation and purification technology, applied in the production of bulk chemicals, etc., can solve the problems of accelerated degradation of active ingredients, long extraction time by enzymatic hydrolysis, and high price of active enzymes, and achieves reduction of gelatinization and degradation. The effect of high survival rate

Inactive Publication Date: 2020-02-07
ZHEJIANG LIZIYUAN FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above production process, the extraction process is very important. Due to the poor stability of polysaccharides, high temperature or long-term solution state will accelerate the degradation of active ingredients, and the extraction time of enzymatic hydrolysis is longer, and the price of active enzymes with higher purity is higher. , which increases the production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Mix 10.0g of jujube with water at a material-to-liquid ratio (g / mL) of 1:5, mix well, and perform microwave extraction at a power of 400W for 2 minutes to obtain crude jujube polysaccharide extract; then use supercritical carbon dioxide extraction technology For extraction, the temperature during the process is 50°C, the pressure is 20MPa, the extraction time is 0.5h, CO 2 The flow rate was 6L / min, and the jujube polysaccharide extract was obtained. The extract containing jujube polysaccharide was added to ethanol solution with a volume fraction of 75% to precipitate, the precipitate was centrifuged and dried to obtain relatively pure jujube polysaccharide with a yield of 15.18% and a purity of 45.8%.

Embodiment 2

[0023] Mix 10.0 g of jujube with a solid-liquid ratio (g / mL) of 1:15, mix it with a 10% ethanol solution by volume fraction, stir well, and perform microwave extraction for 4 minutes at a power of 400W to obtain a crude jujube polysaccharide extract; then Use supercritical carbon dioxide extraction technology for extraction, the temperature in the process is 50°C, the pressure is 20MPa, the extraction time is 1.0h, CO 2 The flow rate was 6L / min, and the jujube polysaccharide extract was obtained. The extract containing jujube polysaccharide was added to ethanol-water solution with a volume fraction of 85% for precipitation, the precipitate was centrifuged and then dried to obtain relatively pure jujube polysaccharide with a yield of 17.53% and a purity of 48.6%.

Embodiment 3

[0025] Mix 10.0 g of red dates with a solid-to-liquid ratio (g / mL) of 1:10, mix them with an ethanol solution with a volume fraction of 20%, stir well, and perform microwave extraction at a power of 600W for 2 minutes to obtain a crude extract of red date polysaccharides; Then use supercritical carbon dioxide extraction technology for extraction, the temperature in the process is 60°C, the pressure is 20MPa, the extraction time is 1.0h, CO 2 The flow rate was 6L / min, and the jujube polysaccharide extract was obtained. The extract containing jujube polysaccharide was added to 80% ethanol-water solution for precipitation, centrifuged to obtain precipitate and then dried to obtain relatively pure jujube polysaccharide with a yield of 17.29% and a purity of 52.3%.

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Abstract

The invention provides a method for separating and purifying jujube polysaccharide from plants. The raw materials containing jujube polysaccharide are added with water or ethanol solution in a material-liquid ratio of 1:1-1:30, a formed mixture is fully mixed uniformly, and microwave extraction is carried out under a microwave power of 100-800W for 1-30min to obtain crude extract of jujube polysaccharide; under the conditions that the extraction temperature is 30-80 DEG C, the extraction pressure is 16-30MPa, the extraction time is 0.5-3h, and the CO2 flow rate is 5-8L / min, the crude jujube polysaccharide extract is subjected to extraction by using supercritical carbon dioxide extraction technology to obtain jujube polysaccharide extraction liquid, ethanol solution with the volume fractionof 60%-99% is added into the jujube polysaccharide extraction liquid, and then the mixture is subjected to centrifuging to obtain a precipitate, and the precipitate is dried to obtain jujube polysaccharide.

Description

technical field [0001] The invention relates to a method for separating and purifying natural products, in particular to a method for rapidly separating and purifying jujube polysaccharide from plants. Background technique [0002] Red jujube polysaccharide is one of the main chemically active components of the fruit of the jujube tree of the rhamnaceae jujube plant, a class of natural macromolecular compounds formed by the condensation of multiple single molecules. According to literature reports, jujube polysaccharides can regulate the immune system at multiple levels and in multiple ways, have obvious anti-complement activity and promote the proliferation of lymphocytes, and can significantly slow down aging. Jujube polysaccharides also have functions such as anti-virus, lowering blood fat, lowering blood sugar, and scavenging free radicals. [0003] Jujube polysaccharides can be mainly extracted from jujube, and the ultrasonic cavitation effect can shorten the extractio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003Y02P20/54
Inventor 郑宋友王平何宇琴王顺余何建新
Owner ZHEJIANG LIZIYUAN FOOD CO LTD
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