Method for extracting wheat total RNA
A wheat and centrifuge tube technology, applied in the field of plant biology, can solve problems such as unsatisfactory extraction results, and achieve the effects of reducing pollution levels, shortening extraction time, and avoiding interference.
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[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.
[0035] See figure 1 , A method for extracting total RNA from wheat, including the following steps:
[0036] Step 1: Take fresh or preserved wheat tissue in a refrigerator at -70°C, add liquid nitrogen in a mortar and grind it with a pestle to powder; add liquid nitrogen during the grinding process to keep the powder in a frozen state; obtain wheat powder;
[0037] Step 2: Move the wheat powder to a 1.5ml centrifuge tube A, add 1ml of ice-co...
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