Gene transfection system and method

Abstract

The invention is applicable to the field of cytobiology, and provides a gene transfection system and method. The gene transfection system comprises a sound-induced heating module and a signal generation module, wherein the sound-induced heating module comprises a piezoelectric substrate, a sound-heat chip arranged on the piezoelectric substrate and N sound absorbing cavities formed in the sound-heat chip, the sound absorbing cavities are used for culturing recipient cells, and N is an integer larger than or equal to 1; the signal generation module is used for outputting a base frequency signal; and the sound-heat chip is used for converting the base frequency signal to produce a sound wave signal, establishing a temperature gradient field according to the sound wave signal, and controllingtemperature of recipient cells in the sound absorbing cavities according to the temperature gradient field to enable the recipient cells to produce a hot piercing effect. The gene transfection systemand method can overcome defects of difficulty in discharging outside, high energy, high toxicity and cell type limitation of a non-viral vector gene transfection method, and can reduce the technicalcost so as to improve practicability and usability of gene therapy.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a gene transfection system and method. Background technique [0002] Gene therapy is a biomedical high technology that introduces human normal genes or genes with therapeutic effects into human target cells in a certain way to correct gene defects or exert therapeutic effects, so as to achieve the purpose of treating diseases. It can be seen that how to accurately and efficiently insert foreign genes into appropriate recipient cells of patients through gene transfer technology is very important. At present, the most commonly used non-viral vector gene transfection methods include electroporation, cationic liposome LipofectamineTM2000, sonoporation and photoporation, among which electroporation and cationic liposome LipofectamineTM2000 are the most commonly used. [0003] However, the ability of cells with different growth characteristics and in vitro culture conditions to accept exog...

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Problems solved by technology

[0004] The main purpose of the present invention is to propose a gene transfection system and method to solve the disadvantages of difficult excretion, high energy, high toxicity and cell type limitations in the gene transfection method in the prior art, so that gene therapy can be used in practice. Problems that cannot be applied well in the application

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