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Method for extracting dioscorea zingiberensis pigment by enzymatic method

A technology of enzymatic extraction and turmeric pigment, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problem of low extraction efficiency, and achieve the advantages of reducing environmental pollution, increasing extraction amount, and improving comprehensive utilization rate. Effect

Inactive Publication Date: 2010-05-19
SHAANXI UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The existing technology of turmeric pigment extraction at present has: Tang Xuan (extraction process research of yellow pigment in turmeric) etc. adopts organic solvent to extract yellow pigment in turmeric; Yellow pigment and the stability of the pigment has been studied; the existing methods for extracting turmeric pigment are all organic solvent extraction methods, and the extraction efficiency is not high

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  • Method for extracting dioscorea zingiberensis pigment by enzymatic method

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Effect test

Embodiment 1

[0019] Embodiment 1: First, get fresh turmeric and dry it and pass it through a 60-mesh sieve after being crushed; secondly, get 60 mg of pectinase and dilute it to 10 mL with an acetic acid-sodium acetate buffer solution with a pH value of 3.6, and prepare 6 mg / mL of pectinase. Gelase enzyme solution: take 1.000g turmeric powder, add 1.5mL of the above pectinase enzyme solution, and make up to 8mL with acetic acid-sodium acetate buffer solution with a pH value of 3.6, react at 40°C for 5h, and then add 12mL of absolute ethanol , extracted in a constant temperature water bath at 65°C for 2.5h, wherein the ratio of solid to liquid was 1g: 20mL, repeated extraction 2 to 3 times, combined the filtrate, and then concentrated the filtrate to an ethanol concentration of less than 10% to obtain turmeric. figure 1 It is the obtained turmeric pigment of the present embodiment through dilution gained ultraviolet spectrum, and the absorption peak of pigment is at 275nm.

Embodiment 2

[0020] Embodiment 2: First, get fresh turmeric and dry it and pass it through a 60-mesh sieve after crushing; secondly, get 60 mg of pectinase and dilute it to 10 mL with an acetic acid-sodium acetate buffer solution with a pH value of 4.0, and prepare 6 mg / mL of pectinase. Gelase enzyme solution: take 1.000g turmeric powder, add 1.5mL of the above pectinase enzyme solution, and make up to 8mL with acetic acid-sodium acetate buffer solution with a pH value of 4.0, react at 45°C for 4h, then add 12mL of absolute ethanol , extracted in a constant temperature water bath at 68°C for 2.3h, wherein the ratio of solid to liquid was 1g: 20mL, repeated extraction 2 to 3 times, combined the filtrate, and then concentrated the filtrate to an ethanol concentration of less than 10% to obtain turmeric.

Embodiment 3

[0021] Embodiment 3: First, get fresh turmeric and dry it and pass it through a 60-mesh sieve after crushing; secondly, take 60 mg of pectinase and dilute it to 10 mL with an acetic acid-sodium acetate buffer solution with a pH value of 4.4, and prepare 6 mg / mL of pectinase. Glue enzyme enzyme solution: take 1.000g turmeric powder, add 1.5mL of the above pectinase enzyme solution, and make up to 8mL with acetic acid-sodium acetate buffer solution with a pH value of 4.4, react at 50°C for 3h, and then add 12mL of absolute ethanol , extracted in a constant temperature water bath at 70°C for 2 hours, wherein the ratio of solid to liquid was 1g: 20mL, repeated extraction 2 to 3 times, combined the filtrate, and concentrated the filtrate until the ethanol concentration was less than 10% to obtain turmeric.

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Abstract

The invention disclose a method for extracting dioscorea zingiberensis pigment by an enzymatic method, which comprises the following steps of: dissolving pectinase in an acetic acid-sodium acetate solution to prepare a pectinase enzymatic solution; adding dioscorea zingiberensis powder into the pectinase enzymatic solution, then adding the acetic acid-sodium acetate solution for reacting, and adding anhydrous ethyl alcohol; extracting in thermostatic waterbath with the temperature of 65-70 DEG C; repeatedly extracting; merging filtrate; and then concentrating the filtrate to that the concentration of ethyl alcohol is less than 10 percent to obtain the dioscorea zingiberensis pigment. By utilizing the special property of the pectinase, the invention effectively improves pigment extraction amount without environmental pollution during production and lays a foundation for the scale production of the dioscorea zingiberensis pigment. By adopting enzyme to pretreat the dioscorea zingiberensis for damaging cell structures and degrading impurities, the invention has higher pigment obtaining efficiency and pigment quality, and not only alleviates environmental pollution and improves the utilization rate of the dioscorea zingiberensis, but also explores a novel edible natural pigment.

Description

technical field [0001] The invention relates to a method for extracting turmeric pigment, in particular to a method for enzymatically extracting turmeric pigment. Background technique [0002] Turmeric, also known as Dioscorea zingiberensis, is a herbaceous vine whose rhizome is yellow or orange and contains starch, tannins, alkaloids, pigments, saponins, etc. It is a classic medicinal material. Turmeric has a long history as medicinal and edible at home and abroad, and its pigment belongs to the list of natural pigments. However, people have paid great attention to the extraction of saponin, a pharmaceutical intermediate from turmeric, for a long time, and the development of turmeric pigment There are not many reports on the use of turmeric pigment. Most saponin production enterprises discharge turmeric pigment with wastewater, which not only pollutes the environment with high chroma of wastewater, but also causes waste of resources. This can provide new natural pigments f...

Claims

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Application Information

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IPC IPC(8): C09B61/00
Inventor 陈合李世玉舒国伟王利红余建军
Owner SHAANXI UNIV OF SCI & TECH
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