Detection kit for plasminogen activator inhibitor and preparation method
A detection kit and plasminogen technology, applied in the biological field, can solve problems such as poor sensitivity and linear range, and achieve the effects of improving sensitivity, improving linear range, and improving repeatability
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Embodiment 1
[0051] A detection kit for plasminogen activator inhibitors, comprising reagent R1 and reagent R2, said reagent R1 comprising buffer, electrolyte, stabilizer, surfactant, accelerator and preservative, said buffer is Concentration is the mixed solution of sodium dihydrogen phosphate dihydrate of 1.6g / L and the disodium hydrogen phosphate dodecahydrate of 8g / L, and described electrolyte is the sodium chloride that concentration is 5g / L, and described stabilizing agent is Concentration is the bovine serum albumin of 1g / L, and described surfactant is the Triton-100 that concentration is 1ml / L, and described accelerator is the polyethylene glycol 6000 that concentration is 60g / L and concentration is 0.5ml / L Proclin950.
[0052] The components and concentrations of the reagent R2 include: latex microsphere antibody conjugates, whose concentration is 0.05%, MES as a coupling buffer, whose concentration is 8g / L, bovine serum albumin as a blocking solution, Its concentration is 20g / L...
Embodiment 2
[0071] A detection kit for plasminogen activator inhibitors, comprising reagent R1 and reagent R2, said reagent R1 comprising buffer, electrolyte, stabilizer, surfactant, accelerator and preservative, said buffer is Concentration is the mixed solution of the 4-hydroxyethylpiperazineethanesulfonic acid of 3g / L and the disodium hydrogen phosphate dodecahydrate of 16g / L, and described electrolyte is the sodium chloride that concentration is 16g / L, and described The stabilizer is bovine serum albumin at a concentration of 3g / L, the surfactant is polyvinylpyrrolidone at a concentration of 3ml / L, the accelerator is polyethylene glycol 6000 at a concentration of 100g / L and the concentration is 1.2 ml / L of Proclin300.
[0072] The components and concentrations of the reagent R2 include: latex microsphere antibody conjugates, whose concentration is 0.4%, MES as a coupling buffer, whose concentration is 18g / L, bovine serum albumin as a blocking solution, Its concentration is 35g / L, and...
Embodiment 3
[0091] A detection kit for plasminogen activator inhibitors, comprising reagent R1 and reagent R2, said reagent R1 comprising buffer, electrolyte, stabilizer, surfactant, accelerator and preservative, said buffer is Concentration is the mixed solution of the sodium dihydrogen phosphate dihydrate of 4.8g / L and the 4-hydroxyethylpiperazineethanesulfonic acid of 24g / L, and described electrolyte is the sodium chloride that concentration is 27g / L, and described Stabilizer is the bovine serum albumin that concentration is 5g / L, and described tensio-active agent is the octylphenyl polyoxyethylene ether that concentration is 5ml / L, and described accelerator is the polyethylene glycol that concentration is 120g / L 6000 and the concentration is 2.0ml / L of thimerosal.
[0092] The components and concentrations of the reagent R2 include: latex microsphere antibody conjugates, whose concentration is 0.75%, MES as a coupling buffer, whose concentration is 27g / L, bovine serum albumin as a blo...
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Abstract
Description
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Application Information
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