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Bacillus natto ADT and application thereof

A technology of Bacillus natto and bacterial agent, applied in the field of microbial environment, can solve problems such as rapid deterioration of sediment and water pollution, decline in self-healing ability, endangering the health of residents, etc., achieve good development and application prospects, reduce adverse effects, adaptable effect

Active Publication Date: 2020-02-14
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ecosystem in the water body is destroyed, and the self-healing ability declines. With the continuous discharge of pollutants, the sediment and water pollution deteriorate and accumulate rapidly, and the difficulty of treatment increases, which seriously affects the living environment of residents and endangers their health.

Method used

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  • Bacillus natto ADT and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Isolation, screening and identification of bacterial strains

[0019] The mass ratio of raw materials for the extraction method provided by the invention: wheat straw, iodine-free salt and high-purity water is 10:2:3.

[0020] Extraction method: Divide the non-iodized salt into two parts for later use; add one part of the non-iodized salt and high-purity water to the wheat straw, grind it thoroughly, add the other part of the non-iodized salt after grinding, and adjust the temperature to 25-30 ° C for 24 hours. Prepare sample liquid;

[0021] Take 5ml of the sample solution and add it to the sterilized LB medium, incubate at 37°C for 24h, take 5ml of the culture solution and inoculate it into a new sterilized LB medium, repeat this process for 5 generations. The culture solution after taking 5 passages was diluted according to the gradient, and the dilution concentration was 10 -3 、10 -4 、10 -5 、10 -6 、10 -7 , spread diluents of different concentrati...

example example 2

[0029] Example 2 Application of a certain black and odorous water body

[0030] The black and odorous water body used in this example is the water body of a certain river course in Jinan. The diversity of vegetation and animals in this river course is poor; dead fish phenomenon. In some sections of the river, the silt is thick and black and smelly. Take the sample back to the laboratory for testing, the COD is 133.86mg / L, the ammonia nitrogen is 16.22mg / L,

[0031] The bacterial strain was connected from the slant medium to the LB liquid medium, and cultivated at 120r / min at 30°C for 24 hours to make a seed liquid, and 5ml of the Bacillus natto ADT ( Bacillus natto ADT) culture solution was applied to 100ml of the channel sample solution and placed at room temperature. Every time, measure the concentration of COD and ammonia nitrogen. The results are shown in Table 1.

[0032]

[0033]It can be seen from Table 1 that the COD decreased by 42.91%, 65.92%, and 75.25% aft...

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Abstract

The invention belongs to the field of microbial environment, and specifically relates to a Bacillus natto extracted from wheat straw and application in hydroecology restoration. The strain provided bythe present invention is named Bacillus natto-ADT, and was deposited in China Center for Type Culture Collection in September, 2019, with a deposit number of CCTCC M 2019762. When the strain is applied in hydroecology restoration, from the perspective of water quality indicators, organic matter in the water can be degraded, COD and ammonia nitrogen in the water can be effectively reduced, the water quality and environmental sense are improved, and the strain has good prospects of development and application.

Description

technical field [0001] The invention belongs to the field of microbial environment, specifically a kind of Bacillus natto ADT ( Bacillus natto ADT) and its application in water ecological restoration. Background technique [0002] Black and odorous water is a relatively serious environmental problem in recent years. Due to the continuous increase in the number of humans and the rapid development of cities, the discharge of industrial sewage and domestic sewage continues to increase. The sewage that has not been systematically treated is directly discharged into rivers. Exceeding the original degradation capacity of the river, the water quality deteriorates, the transparency decreases, and the water body presents black and odorous phenomena. The ecosystem in the water body is destroyed, and its self-repair ability declines. With the continuous discharge of pollutants, the sediment and water quality pollution deteriorates and accumulates rapidly, and the difficulty of treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07
CPCC02F3/34C02F2103/007C12N1/20C12N1/205C12R2001/07Y02A20/402
Inventor 王勇李佳霖刘宗丽焦绪栋尹皓婵秦松夏文菊郭静静张健
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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