Production method for removing IgG from bovine serum
A production method and serum technology, which is applied in the field of immunoglobulin separation, can solve the problems of high IgG content, incapable of large-scale production, and low bovine serum production, and achieve the effects of low glucose content, simple operation, and improved removal rate
Active Publication Date: 2020-02-18
内蒙古维克生生物技术股份有限公司
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[0008]
In order to solve the technical problems of low bovine serum yield and high IgG content in the prior art, which cannot be produced on a large scale, the present invention provides a production method for removing IgG from bovine serum
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[0027] In order to enable those skilled in the art to better understand the technical solutions of the present application, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the implementation of the present application. For example, based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application.
[0028] Select newborn bulls that have not eaten (within 14 hours of birth), and check the files of the calves: including the age, weight, health status of the dam, whether there has been an epidemic in the past year, the vaccinations in the past year, and the weight of the calves, etc. The above-mentioned content is registered in the blood file of the calf to be blood-collected.
[0029] The selected calves to...
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The invention provides a production method for removing IgG from bovine serum. The production method comprises the following steps: performing prefiltration through gauze, performing filtration through a ceramic compound membrane having a pore diameter of 0.5[mu]m, and performing filtering through a hydroxyethyl cellulose modified polyamide micropore filter compound membrane bonded with a phenylalanine affinity ligand; performing circulating ultrafiltration through a hollow fiber ultrafiltration column having a molecular weight cut off of 150kD; forming a macro-molecular group through adsorptive bonding of adsorbent and IgG having small molecular weight, performing ethanol precipitation, adding a high-selectivity adsorbent into the mixture, and further flocculating the small-molecular IgG;and finally, performing filtering and sterilizing to obtain IgG removed bovine serum. According to the layer-by-layer filtering and removing method, the IgG removal rate can be greatly creased to 90percent or more, and fibrous protein and the like can be effectively removed. The method for removing IgG from serum is simple, convenient and rapid, can be used for effectively removing IgG and fibrous protein in bovine serum through layers of filtration, adsorption and chromatography, is stable in process and is applicable to mass production.
Description
technical field [0001] The invention relates to a method for separating immunoglobulins, in particular to a method for producing IgG from bovine serum. Background technique [0002] Human and animal blood is rich in proteins and enzymes that can heal and save lives. Some proteins are in red blood cells and some are in serum. Since the middle of the last century, the purification and separation of proteins has become an important direction of research. So far, the proteins that can be separated include: albumin, lipoprotein, fibrin, immunoglobulin G (IgG) and so on. [0003] Newborn bovine serum is the most important cell culture medium for vaccine production, and it is also an important excipient for enzyme immunoassay kits, and the market demand is huge. In my country, newborn bovine serum is the serum made by aseptically collecting arterial blood from young bulls who have not eaten (within 14 hours of birth). ml, clear appearance. The newborn bovine serum imported from...
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CPCC12N5/0018
Inventor 张永明李世明田元元王旭王鑫
Owner 内蒙古维克生生物技术股份有限公司