K-ras gene mutation site detection kit

Abstract

The invention relates to the field of molecular biotechnology and gene detection, in particular to a K-ras gene mutation site detection kit, comprising: a), a primer pair; b), an upstream probe, downstream probes, and a hairpin probe; and c), two nanogold probes. The above-mentioned primers and probes are suitable for carrying out high-sensitivity high-resolution low-cost detection on K-ras gene mutation sites under closed tube condition, so that cross contaminations to amplification products can be effectively avoided can be effectively avoided, wherein the downstream probes are suitable forindividual use and also for use with each other to carry out multiple detection in a same reaction system, so that the kit herein is suitable for detecting up to seven K-ras gene mutation sites at thesame time, and the detection efficiency is higher.

Description

technical field

[0001] The invention relates to the fields of molecular biotechnology and gene detection, in particular to a K-ras gene mutation site detection kit. Background technique

[0002] K-ras is a small molecular G protein downstream of the EGFR signaling pathway. After mutation, its own GTPase activity is inhibited, so that the K-ras protein is always activated, and the signaling pathway is not regulated by the upstream EGFR signaling instructions. The mutation rate of K-ras gene in patients with colorectal cancer is about 40%, 70% occurs in the 12th codon, and 30% occurs in the 13th codon. K-ras gene mutation status is related to the curative effect of cetuximab, and cetuximab treatment is ineffective when K-ras gene mutation occurs. Drug regimen is very necessary. However, under normal circumstances, in actual clinical samples, the mutated gene is usually mixed with a large number of wild-type gene sequences, and because the sequences between the two are often ...

Images