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Improved Hale colloidal iron staining method and kit for displaying goblet cells

A technology of goblet cell and staining method, which is applied in the field of improved Hale colloidal iron staining method and kit, which can solve the problems of non-specificity of dye and difficulty in accurately judging test results, etc., and achieve good staining effect and easy observation

Inactive Publication Date: 2020-02-25
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the present invention solves the problem that it is difficult to accurately determine the test results due to the nonspecificity of the dye

Method used

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  • Improved Hale colloidal iron staining method and kit for displaying goblet cells
  • Improved Hale colloidal iron staining method and kit for displaying goblet cells
  • Improved Hale colloidal iron staining method and kit for displaying goblet cells

Examples

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Effect test

Embodiment 1

[0081] Example 1 is the result of co-staining of goblet cells and collagen fibers in duodenum specimens after resection of human pancreas and duodenum specimens.

[0082] 1. A staining method and kit for displaying goblet cells, the staining method is: modified Hale colloidal iron staining; the staining kit is: modified Hale colloidal iron staining kit.

[0083] 2. The improved Hale colloidal iron staining kit includes: (1) Solution A: 1000 ml of colloidal iron stock solution (mass production is possible). (2) 1000ml acetic acid solution (mass production available). (3) Liquid B: colloidal iron dye solution, prepared according to the ratio of A: acetic acid solution: distilled water = 4:1:3, the order of preparation of dye solution A, acetic acid solution, and distilled water can be reversed. (4) Liquid C: 1000ml of 5% potassium ferrocyanide aqueous solution (mass production available). (5) Liquid D: 1000 ml of 5% hydrochloric acid aqueous solution (mass production available...

Embodiment 2

[0102] Example 2 is the result of co-staining of goblet cells, collagen fibers, blood vessels, and lymphatic vessels in duodenum specimens after resection of human pancreas and duodenum specimens.

[0103] 1. A staining method and kit for displaying goblet cells, the staining method is: modified Hale colloidal iron staining; the staining kit is: modified Hale colloidal iron staining kit.

[0104] 2. The improved Hale colloidal iron staining kit includes: (1) Solution A: 1000 ml of colloidal iron stock solution (mass production is possible). (2) 1000ml acetic acid solution (mass production available). (3) Liquid B: colloidal iron dye solution, prepared according to the ratio of A: acetic acid solution: distilled water = 4:1:3, the order of preparation of dye solution A, acetic acid solution, and distilled water can be reversed. (4) Liquid C: 1000ml of 5% potassium ferrocyanide aqueous solution (mass production available). (5) Liquid D: 1000 ml of 5% hydrochloric acid aqueous ...

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Abstract

The invention discloses an improved Hale colloidal iron staining method for goblet cells, and is used for displaying the goblet cells in duodenum, mucus, collagen fibers, blood vessels and red blood cells secreted by the goblet cells. The dyeing method has the advantages of strong intuition, distinct dyeing contrast and easy interpretation under a microscope, and the accuracy of test results is ensured. Therefore, the improved Hale colloidal iron staining method for goblet cells solves the problem that the test result is difficult to accurately judge due to the non-specificity of the dye. Theinvention also provides a kit, and the kit is simple in use method and high in practicability and operability.

Description

technical field [0001] The invention belongs to the field of pathological diagnosis and pathological technology in the medical and health field, and can also be used in the fields of biotechnology and scientific research, and relates to an improved Hale colloidal iron staining method and a kit for displaying goblet cells. Background technique [0002] The intestine is an extremely important organ in the human body. It not only has the functions of digesting and absorbing nutrients and producing a variety of hormones, but also has an important immune barrier function. The surface of the intestinal mucosa is covered with a protective mucus gel that interacts with a variety of cells and mechanisms to participate in the maintenance of intestinal homeostasis. Goblet cells are important cells that generate immune responses in the intestinal mucosal immune barrier function. Changes in their differentiation and secretion function can lead to changes in the composition of mucus gel, ...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 丁桂龄郑建明刘艳芳蒋慧韩换何妙侠白辰光戴利和
Owner SHANGHAI CHANGHAI HOSPITAL
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