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Bifidobacterium lactis JYBR-190 capable of improving human immunity and application thereof in food and medicine

A technology of JYBR-190 and bifidobacterium lactis, which is applied in the direction of bifidobacteria, bacteria used in food preparation, and the functions of food ingredients, can solve the problems of unsatisfactory effects and poor effects, and improve the body's immunity strength, reduce disease, and enhance the body's physical fitness

Inactive Publication Date: 2020-02-28
WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the foods currently used to improve the body’s resistance are nutritional products or Chinese herbal medicines purchased in pharmacies. These foods have poor effects on improving the body’s immunity and often do not achieve the desired effect

Method used

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  • Bifidobacterium lactis JYBR-190 capable of improving human immunity and application thereof in food and medicine
  • Bifidobacterium lactis JYBR-190 capable of improving human immunity and application thereof in food and medicine
  • Bifidobacterium lactis JYBR-190 capable of improving human immunity and application thereof in food and medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A Bifidobacterium lactis JYBR-190 strain capable of improving human immunity provided by the invention

[0042] 1. The source of the strain

[0043] The samples come from naturally fermented yoghurt in the homes of herdsmen in Yili Prefecture, Xinjiang;

[0044] 2. Isolation and purification of strains

[0045] 1) Weigh 5 g of naturally fermented yogurt from a herdsman's home in Yili Prefecture, Xinjiang under aseptic conditions, add the yogurt to 45 mL of 0.85% sterilized physiological saline containing glass beads, and mix well to obtain a mother liquor;

[0046] 2) At a rotational speed of 1500 rpm, shake the mother solution for 10 minutes, take 1 mL of the mother solution under sterile conditions and dilute it according to a 10-fold gradient to form a dilution, choose the dilution of the 7th, 8th, and 9th dilution, and take 0.1 Add mL of the diluted solution dropwise to the improved MRS medium plate, smear evenly, make two copies of each dilution, and use normal sal...

Embodiment 2

[0053] Preparation of Bifidobacterium lactis JYBR-190 freeze-dried powder

[0054] The preparation method of the Bifidobacterium lactis lyophilized powder is to inoculate 1% of the Bifidobacterium lactis strains into 99% MRS liquid culture medium by volume percentage, under the condition of 37 ℃, anaerobic culture for 24 hours, and take the MRS Bacteria solution, centrifuged, freeze-dried to obtain bacteria powder, according to the volume ratio of 1:1, add isomalto-oligosaccharide to the bacteria powder, mix evenly to obtain freeze-dried powder of Bifidobacterium lactis, the strain is named JYBR-190, wherein The number of viable bacteria in the described Bifidobacterium lactis JYBR-190 freeze-dried powder is 5 billion / g, 15 billion / g, 25 billion / g, 50 billion / g, 100 billion / g.

Embodiment 3

[0056] Effects of Bifidobacterium lactis JYBR-190 on Cellular Immunity of Mice

[0057] 1. Preparation of bacterial solution

[0058] Inoculate Bifidobacterium lactis JYBR-190 in LB broth medium, culture anaerobically at 37°C for 24 hours, take 10ml of bacterial liquid, wash with sterile PBS buffer for 3 times, and then use McFarland Turbidimetry Adjust the bacterial solution to 10 7 CFU / mL, 10 8 CFU / mL, 10 9 CFU / mL, 10 10 CFU / mL to make 10 7 CFU / mL Bifidobacterium lactis JYBR-190 bacterial solution, 10 8 CFU / mL Bifidobacterium lactis JYBR-190 bacterial solution, 10 9 CFU / mL Bifidobacterium lactis JYBR-190 bacterial solution, 10 10 CFU / mL Bifidobacterium lactis JYBR-190 bacterial solution, for use;

[0059] 2. Carbon particle clearance test

[0060] ①Select 50 mice, weighing 20g, and divide them into 5 groups on average, namely, PBS control group, test group 1, test group 2, test group 3, and test group 4, with 10 mice in each group, for use;

[0061] Experimental gr...

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Abstract

The invention relates to a microbial strain, in particular to bifidobacterium lactis JYBR-190 capable of improving human immunity and application thereof in food and medicine. B ifidobacterium lactisJYBR-190 is collected at the China General Microbiological Culture Collection Center at 3# of Yard 1, Beichenxi Road, Chaoyang District, Beijing on July. 8th, 2019 under the number of CGMCC NO.18092.Immunoregulation effects of bifidobacterium lactis JYBR-190 on bodies mainly include humoral immunity and cellular immunity; particularly, the strain has activity in activating NK cells and phagocytes, can improve erythrocyte immune adhesiveness, can improve body immunity and activation, proliferation and secretion of B lymphocytes through humoral immunity and can improve body immunity through cellular immunity, thereby being capable of effectively improving body immunity of people.

Description

technical field [0001] The invention relates to a microbial strain, specifically a bifidobacterium lactis JYBR-190 capable of improving human immunity and its application in food and medicine. Background technique [0002] Bifidobacterium lactis, also known as Bifidobacterium, is one of the dominant bacteria in the intestinal tract of humans and many mammals. Vitamins B1, B2, B6, etc. can be synthesized in the human intestinal tract, slowly absorbed by the human body, and can also secrete a variety of enzymes to promote the degradation and absorption of food. It can also ferment lactose to produce galactose, which can improve "lactose intolerance" Syndrome" patients' utilization of dairy products. [0003] As the living environment continues to deteriorate, bacteria and viruses often cause some diseases, such as colds, bronchitis, pneumonia, gastroenteritis, etc. Although these diseases are not highly contagious, they cannot be completely isolated from life. These diseases...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A61K35/745A61P37/04C12R1/01
CPCC12N1/20C12N1/04A23L33/135A61K35/745A61P37/04A23V2002/00C12R2001/01C12N1/205A23V2400/531A23V2200/324
Inventor 潘玉林马明磊司书锋曹维超付继先李青青闫洪伟孙川王维新周文浩李翠华裴京广
Owner WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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