Gastrointestinal tract tissue biophoton radiation detection kit and application
A technology for detection kits and tissue biology, which can be used in biological testing, measuring devices, material inspection products, etc., can solve the problem of invisible biophoton radiation, and achieve the effect of wide application value
Pending Publication Date: 2020-02-28
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology
Using our biophoton imaging system (UBIS, authorized invention patent 201310524951.4) to perform long-term imaging of Tyrode's solution perfused gastrointestinal tissue, no biophoton radiation can be observed, but with a concentration of 2 mmol / L and The above 5-HT Tyrode's solution perfusion of gastrointestinal tissue can lead to obvious biophoton radiation
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Embodiment
[0044] Example: Induction of Biophoton Irradiation and Modulation of Mouse Gastrointestinal Tissue
[0045] 1. Test materials: adult Kunming mice, from the Public Service Center for Experimental Animals of Hubei Province.
[0046] 2. Detection content and steps:
[0047] (1) From the isolated tissues of the stomach, duodenum, jejunum, ileum, colon, and rectum of the animal mouse or human digestive tract, cut them into 0.25-1 square millimeters and fix them in double-perfusion tanks, using double-perfusion (2) For gastrointestinal tissue perfusion, use 50ml bench-top solution to circulate the mouse or human gastrointestinal tissue; (3) Use the ultra-weak biophoton imaging system UBIS to detect the gastrointestinal tissue in the perfusion tank Biophotonic imaging of gastrointestinal tissue;
[0048] (1) Different concentrations of reagent A induce changes in the biophoton radiation of mouse gastrointestinal tract tissue
[0049] Take 1ml of deionized water (Tyrode's solution ...
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The invention provides a gastrointestinal tract tissue biophoton radiation detection kit and an application. The detection kit is composed of a reagent A, a reagent B or a reagent C, wherein the reagent A is a gastrointestinal tract tissue biophoton radiation inducer, the reagent B or the reagent C is an action regulation reagent of the reagent A, the reagent A is serotonin, the reagent B is acetylcholine, and the reagent C is norepinephrine. The detection kit is used for evaluating the intensity and characteristics of gastrointestinal tract biophoton radiation, or the reagent of the kit is used as a reference to evaluate the intensity and characteristics of the gastrointestinal tract biophoton radiation independently induced by a to-be-detected substance and the regulation and control effect of the to-be-detected substance on the intensity and characteristics of the gastrointestinal tract biophoton radiation induced by 5-HT; the physiological or pathophysiological action mechanism ofthe to-be-detected substance on gastrointestinal tract tissue is further analyzed, and the detection kit has a high application value in the fields of life science and medical research, new drug development, preparation development, drug effect evaluation and mechanism research of natural and traditional drugs, screening and evaluation of gastrointestinal tract anaphylactic reaction molecules, food safety and the like.
Description
technical field [0001] The invention relates to a detection kit developed based on the principle that 5-HT can induce gastrointestinal tissue biophoton radiation, which belongs to the technical fields of biophotonics, biology, medicine, pharmacy and biomedicine, and can be applied to a wide range of other In the fields of life science and medicine, new drug development, preparation development, efficacy evaluation and mechanism research of natural and traditional drugs, screening and evaluation of gastrointestinal allergic reaction molecules, food safety, etc. Background technique [0002] Five-hydroxytryptamine (5-hydroxytryptamine, 5-HT), also known as serotonin (serotonin), is an important biologically active molecule in the body and also belongs to monoamine neurotransmitters. About 90% of 5-HT in the human body is distributed in the gastrointestinal tract, and only about 10% is distributed in the blood and central nervous system. The 5-HT contained in the gastrointesti...
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IPC IPC(8): G01N33/50
CPCG01N33/5008G01N33/5044
Inventor 戴甲培丁烨彭梦妮张蓓蕾王卓孙燕肖芳艳
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES