Fermented feed protein as well as preparation method and application thereof

A technology for fermenting feed and protein is applied in the field of fermented feed protein and its preparation to achieve the effects of improving fermentation efficiency, increasing neutral protease activity and improving nutritional value

Pending Publication Date: 2020-03-24
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many reports on directly using Moringa oleifera leaves as feed or using Moringa oleifera leaves to make silage, but there are fewer reports on the development and utilization of Moringa oleifera seed dregs for solid-state fermentation to produce feed protein

Method used

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  • Fermented feed protein as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Take 54 parts by mass of Moringa oleifera seed meal, add 46 parts by mass of water, inoculate 16 parts by mass of mixed bacteria of Rhizopus oryzae A and Aspergillus niger A (the ratio is 1:1 by mass), ferment at 30°C, and ferment in a sealed container for 38 hours; then inoculate excrement 8 parts by mass of Enterococcus A, mixed evenly, fermented at 30° C., sealed and fermented for 34 hours. The crude protein content, acid soluble protein content and neutral protease activity of the feed protein prepared by the above method are 40.61%, 9.34% and 752.35U / g respectively.

Embodiment 2

[0070] Take 75 parts by mass of Moringa oleifera seed meal, add 25 parts by mass of water, inoculate 14 parts by mass of mixed bacteria of Rhizopus oryzae A and Aspergillus niger A (the ratio is 1:1 by mass), ferment at 30°C, and ferment in a sealed container for 40 hours; then inoculate excrement 6 parts by mass of Enterococcus A, mixed uniformly, fermented at 30° C., sealed and fermented for 32 hours. The crude protein content, acid-soluble protein content and neutral protease activity of the feed protein prepared by the above method are 41.88%, 9.64% and 849.74U / g respectively.

Embodiment 3

[0072] Take 50 parts by mass of Moringa oleifera seed meal, add 50 parts by mass of water, inoculate 13 parts by mass of Rhizopus oryzae A and Aspergillus niger A mixed bacteria (ratio: 1:1 by mass), ferment at 30°C, and ferment in a sealed container for 36 hours; then inoculate excrement 7 parts by mass of Enterococcus A, mixed evenly, fermented at 30° C., sealed and fermented for 36 hours. The crude protein content, acid-soluble protein content and neutral protease activity of the feed protein prepared by the above method are 46.78%, 10.64% and 711.93U / g respectively.

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Abstract

The invention discloses a fermented feed protein as well as a preparation method and application thereof. The preparation method of the fermented feed protein disclosed by the invention utilizes a stepwise fermentation way which specifically comprises the following steps: fermenting moringa seed meal with aspergillus niger and rhizopus oryzae so as to have macromolecules, including cellulose, proteins and the like, degraded into small molecules; inoculating the fermented moringa seed meal with enterococcus faecium so as to allow direct use of small-molecule substances; and then, further fermenting the moringa seed meal so as to have fermentation efficiency improved, thereby more effectively increasing nutritional values of the moringa seed meal. Being subjected to the fermentation, the moringa seed meal is capable of producing sour and aromatic flavor; and moreover, the fermented moringa seed meal has significantly improved crude protein content, acid-soluble protein content, neutral protease activity and the like compared with moringa seed meal subjected to single-bacterium fermentation. Therefore, the fermented moringa seed meal is rich in probiotics, and becomes a high-quality feed protein.

Description

technical field [0001] The invention belongs to the technical field of feed processing, and in particular relates to a fermented feed protein and its preparation method and application. Background technique [0002] Feed protein is an important resource for aquatic products and livestock breeding. At present, all countries in the world have a great demand for feed protein, coupled with the increase in the price of traditional feed protein such as fish meal, looking for potential, high-quality feed protein is one of the important goals of the current aquaculture industry. Therefore, optimizing feed formula and reducing feed cost are important research issues in the aquaculture industry. Compared with traditional feed protein, fermented feed protein has the advantages of high digestibility and high feed-to-meat ratio. In recent years, there have been more and more researches on the production of new fermented feed protein from agricultural and animal husbandry waste such as s...

Claims

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Application Information

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IPC IPC(8): A23J1/00A23J1/14A23K10/12A23K20/147
CPCA23J1/005A23J1/148A23K10/12A23K20/147
Inventor 吴振强郭志鹏黄文琪
Owner SOUTH CHINA UNIV OF TECH
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