Small peptide for promoting wound healing and application thereof

A wound healing and trauma technology, applied in the biological field, can solve problems such as deficiency, and achieve the effect of promoting repair, promoting migration, and reducing cell infiltration

Active Publication Date: 2020-03-24
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Small peptides that promote wound healing are lacking in the prior art

Method used

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  • Small peptide for promoting wound healing and application thereof
  • Small peptide for promoting wound healing and application thereof
  • Small peptide for promoting wound healing and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The liquid phase and mass spectrogram of embodiment 1 small peptide YDW

[0022] The amino acid sequence of the small peptide YDW is as follows: YDWPGGRN (SEQ ID NO: 1), with a molecular weight of 964.5 Da. The small peptide YDW was purchased from GenScript Biotechnology Co., Ltd.

Embodiment 2

[0023] Example 2 Effect of small peptide YDW on the proliferation of mouse fibroblast L929 and human immortalized epidermal cells HaCaT

[0024] The small peptide YDW was dissolved in RPMI 1640 medium at the final concentrations of 5 μM, 20 μM, and 80 μM, and the effect of small peptide YDW on the proliferation of mouse fibroblast L929 and human immortalized epidermal cells HaCaT was detected by MTT method. The specific method is as follows: Human immortalized epidermal cells HaCaT (Hunan Fenghui Biotechnology Co., Ltd.) and mouse fibroblast L929 (Hunan Fenghui Biotechnology Co., Ltd.) were respectively treated with 10% (volume percentage concentration) FBS ( Fetal bovine serum) RPMI 1640 culture medium was configured to 5×10 4 cells / mL of cell suspension. Add 100 μL of cell suspension to each test well of a 96-well plate in CO 2 Cultivate in the incubator for 12 hours to adhere to the cells, and set up a blank group in which RPMI 1640 culture medium replaces the cell suspen...

Embodiment 3

[0026] Example 3 Small peptide YDW inhibits LPS-induced secretion of NO in macrophage RAW 264.7

[0027] Macrophage RAW 264.7 cells (Hunan Fenghui Biotechnology Co., Ltd.) were prepared in RPMI 1640 culture medium containing 10% FBS (fetal bovine serum) to 5×10 4 cells / mL of cell suspension. Add 400 μL of cell suspension to each test well of a 24-well plate in CO 2Cultivate in the incubator for 12 h to make the cells adhere to the wall, and add 5 μM, 20 μM and 80 μM small peptide YDW solutions respectively (the small peptide YDW solution is obtained by dissolving small peptide YDW in RPMI1640 culture medium supplemented with 10% FBS ), after 2 h, add LPS (lipopolysaccharide, company Solarbio, product number L8880), and place in the incubator at constant temperature for 24 h. In addition, control wells and blank wells were set up. In the control wells and the blank wells, the RPMI1640 culture solution supplemented with 10% FBS was used to replace the small peptide YDW solutio...

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PUM

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Abstract

The invention provides a small peptide for promoting wound healing and application thereof, and belongs to the technical field of biology. The amino acid sequence of the small peptide for promoting wound healing is shown as SEQ ID NO: 1. The invention provides application of the small peptide in preparation of medicines for treating traumatic skin injury, and an application in preparation of beautifying skin-nourishing products. The invention also provides the medicines for treating traumatic skin injury, and the beautifying skin-nourishing products, which contain the small peptide for promoting wound healing. The small peptide provided by the invention can significantly promote wound healing.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a small peptide for promoting wound healing and its application. Background technique [0002] The skin is the body's first line of defense against external damage. Chronic or inappropriate wound healing endangers the quality of life of the population, and there is a risk of chronic skin ulceration and complications. Once the skin is damaged, the body will immediately adjust to repair the damage. The process of wound healing is dynamic and includes three highly integrated and overlapping phases: (1) the inflammatory response phase, including the extravasation of blood components leading to platelet aggregation, coagulation, and migration of inflammatory cells to the wound; (2) the proliferative phase, including the cutin Migration and proliferation of forming cells, fibroblasts and endothelial cells, leading to the formation of regenerated epithelium and granulation tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P17/02A61K8/64A61Q19/00
CPCC07K7/06A61P17/02A61K8/645A61Q19/00A61K38/00
Inventor 沈新春隋慧琳汪芳
Owner NANJING UNIV OF FINANCE & ECONOMICS
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