A tilapia ghrelin mature peptide and its expression and application
A ghrelin and tilapia technology, applied in the fields of biochemistry and molecular biology, can solve the problems of unseen ghrelin mature peptides, etc., to protect against Aeromonas hydrophila infection, enhance immunity and disease resistance , the effect of strong stability
Inactive Publication Date: 2021-07-27
GUANGXI ACADEMY OF FISHERY SCI
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Problems solved by technology
There is no application of Ghrelin mature peptide in improving the immune function of tilapia
Method used
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Embodiment 1
[0037] 1. Synthesis of Tilapia Ghrelin Mature Peptide
[0038] The synthesis sequence of the tilapia ghrelin mature peptide of the present invention is GSSFLSPSQKPQNKVKSSRIGR, the crude tilapia ghrelin mature peptide is synthesized according to the Fmoc-solid-phase peptide synthesis method, and the crude tilapia ghrelin mature peptide is purified by chromatography. The tilapia ghrelin mature peptide synthesized by the invention is a small molecular polypeptide containing 22 amino acid residues, and the relative molecular mass is 2.39KD.
[0039] The Ghrelin precursor protein of tilapia contains 107 amino acid residues, the first 26 amino acids at the N-terminal are signal peptides, the mature peptide of Ghrelin is 22 amino acids, starting from the 27th amino acid, directly connected to the signal peptide, and the 59 amino acids at the C-terminal amino acid is the C-terminal peptide.
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The invention discloses a tilapia ghrelin mature peptide and its expression application, belonging to the fields of biochemistry and molecular biology. The synthetic sequence of the tilapia ghrelin mature peptide of the present invention is GSSFLSPSQKPQNKVKSSRIGR, according to the Fmoc-solid-phase polypeptide synthesis method, the crude product of the tilapia ghrelin mature peptide is synthesized and purified by chromatography, and the purity of the obtained tilapia ghrelin mature peptide is 90 -95%. The present invention injects tilapia with the synthesized tilapia Ghrelin mature peptide, regulates the gene expression of tilapia HSP70, IL-1β, TGF-β and TNF-α, and prevents tilapia from being infected with Aeromonas hydrophila .
Description
technical field [0001] The invention belongs to the field of biochemistry and molecular biology, and in particular relates to a tilapia ghrelin mature peptide and its expression application. Background technique [0002] Ghrelin is a novel peptide hormone isolated from rat gastric mucosa, containing 28 amino acid residues, which activates the Neuropeptide Y / agouti-related protein neurons stimulate the expression of neuropeptide Y (NPY) and agouti-related protein (AGRP) to promote food intake and regulate endocrine hormones. In addition, ghrelin is effective in anti-inflammation and mainly acts on the immune regulation function of cytokines. The main way of its anti-inflammatory effect is to inhibit the expression of pro-inflammatory factors. Previous research results have shown that in addition to regulating fish appetite and growth, ghrelin also has a regulatory effect on the immune system. [0003] In fish, Ghrelin protein is mainly expressed in gastric tissue. Ghrelin...
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IPC IPC(8): C07K14/575C12Q1/6888A61K38/22A61P31/04
CPCA61K38/00A61P31/04C07K14/575C12Q1/6888C12Q2600/166
Inventor 钟欢周毅韩卓君罗永巨唐瞻杨肖俊郭忠宝郑惠芳
Owner GUANGXI ACADEMY OF FISHERY SCI