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Enterococcus lactis AR from chicken as well as screening method and application thereof

A technology of Enterococcus lactis and a screening method, applied in the field of veterinary probiotics, can solve the problems of low safety of antibiotics and poor treatment effect, and achieve the effects of improving immunity of the body, improving bacterial salpingitis, and strong adaptability

Active Publication Date: 2020-03-24
HEBEI KEXING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a chicken-derived Enterococcus lactic acid AR, in order to solve the problem of low safety in the use of antibiotics and poor therapeutic effect in the use of other preparations;

Method used

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  • Enterococcus lactis AR from chicken as well as screening method and application thereof
  • Enterococcus lactis AR from chicken as well as screening method and application thereof
  • Enterococcus lactis AR from chicken as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Chicken-derived Enterococcus lactic acid AR

[0046] Enterococcus lactis of chicken origin ( Enterococcus lactis ) AR, which was isolated and screened from the contents of hen oviduct, and was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on September 10, 2019. The preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, the deposit number is CGMCC No. 18483, and the Latin name is Enterococcus lactis .

[0047] Its 16SrRNA sequence is as follows:

[0048] CGGGCGGTGTGTAACTGCCCGGTAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCGGCTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGAGAAGCTTTAAGAGATTAGCTTAGCCTCGCGACTTCGCAACTCGTTGTACTTCCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTTGCTAGAGTGCCCAACTGAATGATGGCAACTAACAATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCACTTTGCCCCCGAAGGGGAAGCTCTATCTCTAGAGTGGTCAAAGGATGTCAAGACCTGGT...

Embodiment 2

[0052] Example 2 Screening method for chicken-derived Enterococcus lactic acid AR

[0053] This embodiment is a screening method for chicken-derived Enterococcus lactic acid AR involved in Example 1, which includes the following steps in sequence:

[0054] 1) It is taken from the oviduct of a hen in a healthy laying period under aseptic conditions, and sealed and preserved under aseptic conditions after being taken out;

[0055] Under aseptic conditions, take the content in the oviduct of the hen and place it in a sterile anaerobic incubator for standby;

[0056] 2) Inoculate the above contents in A 2 On the culture medium, A 2 The medium is tryptone soybean agar medium (TSA medium);

[0057] 3) The inoculated A 2 ’ Culture medium was placed in an anaerobic incubator at 37°C for 48 hours to obtain culture B 1 ; The anaerobic environment in the anaerobic incubator consists of H with a volume ratio of 10:5:85 2 , CO 2 and N 2 composition;

[0058] 4) In culture B 2 Pic...

Embodiment 3-7

[0061] Example 3-7 Screening method for chicken-derived Enterococcus lactic acid AR

[0062]Examples 3-7 are the screening methods of chicken-derived Enterococcus lactic acid AR respectively, the screening method is the same as that of Example 2, the difference is that some conditional parameters in the screening process are different, see Table 1 for details:

[0063] Table 1 List of different conditions and parameters for screening Enterococcus lactic acid AR from chicken

[0064]

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Abstract

The invention discloses Enterococcus lactis AR from chicken and a preparation method thereof. The invention belongs to the field of probiotics for livestock. The Enterococcus lactis AR from chicken ispreserved in China General Microbiological Culture Collection Center; the preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing; the strain is named as Enterococcus lactis, the preservation date is September 10, 2019, the preservation number is CGMCC No.18483, and the name of Latin is Enterococcus lactis, the problems that the safety is low when antibiotics are used,and the treatment effect on hen salpingitis is poor when other preparations are used are solved; the invention further discloses a screening method of the Enterococcus lactis AR from chicken. The Enterococcus lactis AR from chicken is obtained by separating and screening healthy hen oviduct. The invention also discloses an application of the Enterococcus lactis AR from chicken in improving salpingitis of hens. The Enterococcus lactis AR from chicken can be colonized in chicken fallopian tubes to inhibit and remove pathogenic bacteria; the medicine is suitable for improving salpingitis of hens.

Description

technical field [0001] The invention belongs to the field of veterinary probiotics, and relates to a poultry native bacterium, in particular to a chicken-derived Enterococcus lactic acid AR and a screening method and application thereof. Background technique [0002] The world's first report on the growth-promoting effect of antibiotics was published in 1946, confirming that the addition of penicillin to the diet can improve the production performance of poultry. Since then, subclinical doses of antibiotics have been widely used to improve animal production performance and feed conversion efficiency. At the same time, the harm caused by the long-term and large-scale use of antibiotics has gradually been exposed; subclinical doses of antibiotics can cause bacterial resistance, and drug resistance genes It can be transferred from animals to humans, seriously threatening human health. In 1986, Sweden became the first country to ban antibiotics as animal growth promoters; in 20...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A61K35/744A61P15/08A61P31/04C12R1/01
CPCC12N1/20C12N1/02A61K35/744A61P15/08A61P31/04C12R2001/46C12N1/205
Inventor 杨锴陈淑芳牛志勇石虎
Owner HEBEI KEXING PHARMA
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