Biological detection device adopting gold nanopore array chip and detection method

Abstract

The invention provides a biological detection device adopting a gold nanopore array chip and a detection method. The biological detection device adopting the gold nanopore array chip comprises a lightsource, a sample loading unit, a photoelectric detector and a processor, wherein the sample loading unit comprises a sample loading micropore for accommodating a sample to be detected, and a gold nanopore array chip is arranged at the bottom of the sample loading micropore; a plurality of nanopores are formed in the surface of one side of the gold nanopore array chip; the light source is used forirradiating the sample loading unit added with a sample to be detected, and the photoelectric detector receives light rays transmitted from the sample loading unit and transmits the light rays to theprocessor.

Description

technical field [0001] The invention belongs to the technical field of biological detection; more specifically, the invention relates to a biological detection device and a detection method using a gold nanohole array chip. Background technique [0002] Biological detection is a method that uses the sensitive response of organisms to substances for determination. The applications of biological detection are very wide. For example, amyloid aggregates into amyloid fibrils through misfolding and deposits in various tissues and organs in the human body. For the detection of this, ThT fluorescence detection technology is currently mainly used to monitor the aggregation of amyloid protein. However, the current methods are difficult to achieve the advantages of less sample loading, high sensitivity, wide detection line, fast test speed, and good stability. [0003] For another example, the minimum concentration of amphiphilic polymers in a solvent to form micelles or nanoparticl...

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Problems solved by technology

Advantages and disadvantages of different monitoring methods: the light scattering method requires the measured solution to be very clean, and there are many limiting factors; while the conductivity method is only suitable for measuring the CMC of ionic surfactants; the fluorescent probe method is used to measure CMC, although there are few limiting factors , but the benzene solution of the pyrene probe needs to be configured, and benzene is toxic and the operation process is cumbersome; the equipment required for the measurement of CMC by the dye method is simple, but the color change is not obvious enough, which will affect the accuracy of the CMC; Hydrocarbon solubles affect the critical micelle concentration of surfactants, and the accuracy of the measured CMC is affected

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