Unlock instant, AI-driven research and patent intelligence for your innovation.

Recombinant protein and fragments thereof, method for producing said recombinant protein, synthetic gene and use of the recombinant protein

A technology of recombinant protein and synthetic gene, which is applied in the fields of pharmacy, molecular biology, medicinal chemistry, biochemistry and genetics, and can solve problems such as short half-life

Pending Publication Date: 2020-03-31
FUNDACAO BUTANTAN
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of all the leech peptides, bivalirudin, an FDA-approved anticoagulant, is a direct thrombin inhibitor but has a short half-life

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombinant protein and fragments thereof, method for producing said recombinant protein, synthetic gene and use of the recombinant protein
  • Recombinant protein and fragments thereof, method for producing said recombinant protein, synthetic gene and use of the recombinant protein
  • Recombinant protein and fragments thereof, method for producing said recombinant protein, synthetic gene and use of the recombinant protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0073] Here, a novel class of thrombin inhibitors, in particular direct and specific thrombin inhibitors modified from sculptin identified in transcriptome analysis of tick salivary glands, will be described. It consists of 168 residues with four identical repeats and exhibits evolutionary divergence from classical hirudins. The recombinant protein is a competitive, specific and reversible thrombin inhibitor with a K of 18.5±2.2pM. It is slowly digested by thrombin and loses its inhibitory activity. Thus, the recombinant protein is hydrolyzed by Factor Xa, and each polypeptide fragment is capable of inhibiting thrombin in an independent manner. A single domain of the recombinant protein, which retains only about 45% of its inhibitory activity, has been proposed to bind thrombin in a bivalent fashion. Forming a helix / small turn-like structure by incorporating residues from the active site of the recombinant protein domain would make it the most potent thrombin inhibitor compa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention falls within the fields of biochemistry, molecular biology, genetics, pharmacy and medicinal chemistry, being related to biochemical and metabolic processes. More specifically, the present invention provides a novel class of proteins that inhibit thrombin, particularly direct inhibitors of thrombin modified from sculptin, identified in the transcriptomics analysis of the salivary glands of ticks, as well as fragments and recombinant protein thereof, which can be used as anticoagulant agents and for the prophylaxis and / or treatment of thromboembolic diseases.

Description

technical field [0001] The present invention describes a novel thrombin inhibitor protein, in particular a direct inhibitor of thrombin, as well as fragments and recombinant proteins thereof which can be used as anticoagulants. The present invention belongs to the fields of biochemistry, molecular biology, genetics, pharmacy and medicinal chemistry, and is related to biochemistry and metabolic process. Background technique [0002] Thrombin is a multifunctional enzyme with a major function in the blood coagulation cascade. Modulation of its function can lead to normal blood clotting or conditions such as thrombosis. Therefore, it is of great importance to identify new potent and specific thrombin inhibitors. [0003] Blood coagulation is a dynamic process that involves a cascade of zymogen downstream activation. Under normal conditions, it causes homeostasis. It is crucial that the thrombus-hemorrhagic balance is maintained in vivo by complex and controversial mechanism...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/81C12N15/15A61K38/55A61P7/02
CPCC07K14/81A61K38/00C07K2319/21C07K14/811
Inventor A·M·储德金斯基-塔瓦希M·B·戈德费得A·伊克巴尔
Owner FUNDACAO BUTANTAN