Prognosis kit for lymphoma

A kit, technology for lymphoma

Inactive Publication Date: 2020-04-03
蔡清清
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no good method for detecting the progno

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Prognosis kit for lymphoma
  • Prognosis kit for lymphoma
  • Prognosis kit for lymphoma

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1 Preparation of DNA

[0036] 1. Extract DNA from paraffin specimens

[0037] Place the paraffin specimen in a 1ml EP tube, use gradient xylene and alcohol for dewaxing, add 180ul ATL buffer and 20ul proteolytic enzyme, and incubate it in a 56℃ water bath overnight. After the tissue is digested, use 200ul ATL buffer and 200ul ethanol to mix well in a metal bath at 90°C for 1 hour. After centrifugation, transfer the supernatant to the adsorption column. After centrifugation at 13000rpm, centrifuge the liquid in the spin column to the collection tube and discard Collect the collection tube, put the adsorption column in a new collection tube, add 500ul AW1 buffer and centrifuge at 1200rpm for 1 min, then add AW2 500ul and centrifuge for 1 minute, put the adsorption column in a clean 1.5ml EP tube, add 100ul ATEbuffer, and place at 70℃ Centrifuge at 13000rpm for 2 minutes for 10 minutes, add 50ulPBS to 20°C and store.

[0038] 2. Extract DNA from fresh tissue

[0039] The...

Example Embodiment

[0040] Example 2 Prognosis Evaluation Process

[0041] 1. Combine 1 specific sequencing primer and DNA template, and then add 200ul enzyme mixture (DNA, Polymerase, ATP sulfurylase, Luciferase, and Apyrase), and substrate mixture (APS and Luciferin).

[0042] 2. Add 1 dNTP to the reaction system. If it can just match the next base of the DNA template, under the action of DNA polymerase, the 3'end of the sequencing primer will be added, and a molecule of Pyrophosphate (PPi).

[0043] 3. Under the action of ATP sulfurylase, the generated PPi can combine with APS to form ATP; under the catalysis of luciferase, the generated ATP can form oxyluciferin with luciferin, and the specificity can be obtained through the CCD optical system The detected peak value is proportional to the number of matched bases.

[0044] 4. The remaining dNTP and a small amount of ATP in the reaction system are degraded under the action of Apyrase.

[0045] 5. Add another dNTP, repeat the 2-4 reaction, and get the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biomedicines and particularly relates to a prognosis kit for lymphoma. According to the prognosis kit, tissue of a biopsy specimen is subjected to DNA extracting, then, expression levels of methylation of a gene HOXC5, a gene SRPRB, a gene CELF2 and a gene PRR36 are detected by using the prognosis kit for lymphoma, provided by the invention, and then, prognosis of adult T lymphocytes is judged. The invention provides the prognosis kit in view of adult T lymphoblast lymphoma for the first time, the prognosis of adult T lymphoblast lymphoma patients can be effectively predicted, and evaluations with guiding significance can be made for sufferers suitable for being subjected to bone marrow transplantation.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a lymphoma prognosis kit. Background technique [0002] Lymphoma is a malignant tumor originating from the lymphatic hematopoietic system. It is mainly manifested as painless lymphadenopathy, hepatosplenomegaly, and various tissues and organs of the whole body can be involved, accompanied by systemic symptoms such as fever, night sweats, weight loss, and itching. [0003] According to the tumor cells, it is divided into two types: non-Hodgkin's lymphoma (NHL) and Hodgkin's lymphoma (HL). Pathological features In Hodgkin's lymphoma, the tumor tissue contains lymphocytes, eosinophils, plasma cells and specific Reed-Steinberg cells, and HL is divided into nodular lymphoid-rich Cellular and classical, the latter including lymphocyte predominant, nodular sclerosis, mixed cellular and lymphocyte depleted. The incidence of NHL is much higher than that of HL. It is the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/154
Inventor 蔡清清谢丹田小朋
Owner 蔡清清
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap