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Prognosis kit for lymphoma

A kit, technology for lymphoma

Inactive Publication Date: 2020-04-03
蔡清清
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no good method for detecting the prognosis of lymphoma has been found in the prior art.

Method used

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  • Prognosis kit for lymphoma
  • Prognosis kit for lymphoma
  • Prognosis kit for lymphoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1DNA

[0036] 1. DNA Extraction from Paraffin Specimens

[0037] Place the paraffin specimens in 1ml EP tubes, use gradient xylene and alcohol for dewaxing, add 180ul ATL buffer and 20ul proteolytic enzymes, and incubate overnight in a 56°C water bath. After the tissue is digested, put it in a metal bath at 90°C for 1 hour, mix with 200ul ATL buffer and 200ul ethanol, centrifuge and transfer the supernatant to the adsorption column, after centrifugation at 13000rpm, centrifuge the liquid in the spin column into a collection tube and discard Collection tube, put the adsorption column in a new collection tube, add AW1 buffer 500ul and centrifuge at 1200rpm for 1min, then add AW2 500ul and centrifuge for 1 minute, put the adsorption column in a clean 1.5ml EP tube, add 100ulATEbuffer, and place at 70°C For 10 minutes, centrifuge at 13000rpm for 2 minutes, add 50ulPBS and store at 20°C.

[0038] 2. DNA Extraction from Fresh Tissue

[0039] A...

Embodiment 2

[0040] Embodiment 2 prognosis evaluation process

[0041] 1. Combine one specific sequencing primer with the DNA template, and then add 200ul of enzyme mixture (DNA, Polymerase, ATP sulfurylase, Luciferase, and Apyrase), and substrate mixture (APS and Luciferin).

[0042] 2. Add 1 dNTP to the reaction system. If it can just pair with the next base of the DNA template, it will add the 3' end of the sequencing primer under the action of DNA polymerase, and release a molecule of Pyrophosphate (PPi).

[0043]3. Under the action of ATP sulfurylase, the generated PPi can combine with APS to form ATP; under the catalysis of luciferase, the generated ATP can form oxyluciferin with luciferin, and the specificity can be obtained through the CCD optical system. The detection peak, the height of the peak is proportional to the number of matched bases.

[0044] 4. The remaining dNTPs and a small amount of residual ATP in the reaction system were degraded under the action of Apyrase.

[...

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Abstract

The invention belongs to the technical field of biomedicines and particularly relates to a prognosis kit for lymphoma. According to the prognosis kit, tissue of a biopsy specimen is subjected to DNA extracting, then, expression levels of methylation of a gene HOXC5, a gene SRPRB, a gene CELF2 and a gene PRR36 are detected by using the prognosis kit for lymphoma, provided by the invention, and then, prognosis of adult T lymphocytes is judged. The invention provides the prognosis kit in view of adult T lymphoblast lymphoma for the first time, the prognosis of adult T lymphoblast lymphoma patients can be effectively predicted, and evaluations with guiding significance can be made for sufferers suitable for being subjected to bone marrow transplantation.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a lymphoma prognosis kit. Background technique [0002] Lymphoma is a malignant tumor originating from the lymphatic hematopoietic system. It is mainly manifested as painless lymphadenopathy, hepatosplenomegaly, and various tissues and organs of the whole body can be involved, accompanied by systemic symptoms such as fever, night sweats, weight loss, and itching. [0003] According to the tumor cells, it is divided into two types: non-Hodgkin's lymphoma (NHL) and Hodgkin's lymphoma (HL). Pathological features In Hodgkin's lymphoma, the tumor tissue contains lymphocytes, eosinophils, plasma cells and specific Reed-Steinberg cells, and HL is divided into nodular lymphoid-rich Cellular and classical, the latter including lymphocyte predominant, nodular sclerosis, mixed cellular and lymphocyte depleted. The incidence of NHL is much higher than that of HL. It is the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/154
Inventor 蔡清清谢丹田小朋
Owner 蔡清清
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