Tissue exosome phosphorylated proteome-based multi-omics analysis method

A phosphorylated protein and analysis method technology, applied in the field of multi-omics analysis of exosome proteomics and transcriptome, can solve the problems of lack of multi-omics analysis methods, and achieve the effect of strong universality and large amount of information

Active Publication Date: 2020-04-03
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently a lack of multi-omic analysis methods based on tissue exosome phosphorylated proteome
[0006] The present invention is an integrated analysis method...

Method used

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  • Tissue exosome phosphorylated proteome-based multi-omics analysis method
  • Tissue exosome phosphorylated proteome-based multi-omics analysis method
  • Tissue exosome phosphorylated proteome-based multi-omics analysis method

Examples

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Embodiment 1

[0036] In this example, taking lung cancer tissue exosome phosphorylated proteome sequencing data and the combination strategy analysis method of transcriptome and gene variation data as an example, a multi-omics analysis method based on tissue exosome phosphorylated proteome is provided (step Process such as figure 1 shown), consists of the following steps:

[0037] (S1) Design experiments according to requirements, obtain clinical samples, and separate exosomes from tissue samples

[0038]Cancer tissues and paracancerous tissues 5cm away from the cancerous tissues were obtained during the operation of lung cancer patients. The cancer and paracancerous tissues of 13 lung cancer patients were weighed and washed twice with 4°C pre-cooled PBS. Lung tissue was thoroughly lysed in 20 mL of cold PBS by mechanical homogenization. The homogenate solution was then filtered through a 40 μm cell strainer. The filtrate was continuously centrifuged at 4000 × g for 30 min to remove all ...

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Abstract

The invention provides a tissue exosome phosphorylated proteome-based multi-omics analysis method. The method comprises the following steps of: S1, separating exosomes of a tissue sample and specifically enriching phosphorylated proteins; S2, carrying out sequencing analysis on the enriched phosphorylated protein to obtain main regulation kinase; S3, analyzing transcriptome data of the tissue sample to obtain a main regulation transcription factor; S4, analyzing the genome variation data of the tissue sample to obtain a tumor-related gene; and S5, performing strategy analysis on the analysis data obtained in the steps S2, S3 and S4 to obtain a cancer-related main regulation kinase network and a drug action target. The method is suitable for the joint analysis technology of phosphorylated proteome sequencing data, transcriptome data and gene mutation data of any disease exosome sample, and a foundation is laid for exploring markers related to diseases and selecting more effective and accurate disease treatment targets.

Description

technical field [0001] The invention relates to the technical field of proteomics and gene transcriptomics, in particular to a multi-omics analysis method for exosome proteomics and transcriptomics. Background technique [0002] The form of gene expression includes transcriptome and proteome. Analysis of gene expression profiles allows the exploration and identification of underlying molecular and cellular processes. With advances in key high-throughput technologies, such as RNA-sequencing and shotgun proteomics, it is now possible to probe gene transcription and protein expression with unprecedented depth and coverage. The transcriptome and proteome, as well as post-transcriptional and post-translational modifications, are dynamic and tightly regulated in response to different environmental stimuli and growth conditions. While transcriptomes and proteomes each have many bioinformatics approaches, these approaches are limited in their ability to provide a systematically co...

Claims

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Application Information

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IPC IPC(8): G16B20/30G16B30/00C12Q1/6886G01N33/574G01N33/68
CPCG16B20/30G16B30/00C12Q1/6886G01N33/574G01N33/68Y02A90/10
Inventor 肖华乔智张岩
Owner SHANGHAI JIAO TONG UNIV
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