Formula and dilution method of diluent for normal-temperature preservation of Hu sheep semen

A technology for preservation and dilution at room temperature, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of not meeting the actual production needs, short preservation time of Hu sheep semen at room temperature, etc., to avoid the sperm structure. The effect of damage, reduction of breeding cost and improvement of efficiency

Inactive Publication Date: 2020-04-07
YANGZHOU UNIV
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Problems solved by technology

[0005] The object of the present invention is to address the above existing problems, to provide a diluent formula and a dilution method for Hu sheep semen stored at room temperature, which ...

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  • Formula and dilution method of diluent for normal-temperature preservation of Hu sheep semen
  • Formula and dilution method of diluent for normal-temperature preservation of Hu sheep semen
  • Formula and dilution method of diluent for normal-temperature preservation of Hu sheep semen

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Embodiment Construction

[0018] The present invention will be further described below in combination with specific embodiments.

[0019] Materials and Methods;

[0020] 1. Test reagents and consumables

[0021] Tris (A100826-0500), fructose (A100226-0500), citric acid (A100529-0250) and 0.22 μm filter membrane (F513142-0001) were purchased from Sangon Bioengineering (Shanghai) Co., Ltd.; penicillin sodium (S17030-100g ) and streptomycin sulfate (S17058-100g) were purchased from Yuanye Biology (Shanghai) Technology Co., Ltd.; electronic analytical balance (AB204-N) was purchased from Mettler Toledo Measuring Equipment (Shanghai) Co., Ltd.

[0022] 2. Specific operation process;

[0023] Preparation of Hu sheep semen storage dilution at room temperature: Use a balance to accurately weigh 30.7g Tris, 16.4g citric acid, 20g fructose, dissolve in 1000mL sterilized ultrapure water, make it fully dissolved, and filter with a 0.22μm filter membrane Sterilize, store in refrigerator at 4°C, add 0.3118g penic...

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Abstract

The invention provides a formula and a dilution method of a diluent for normal-temperature preservation of Hu sheep semen. According to the invention, Hu sheep semen is subjected to normal-temperaturepreservation by using five diluents, and the motility rate, the vitality, the plasma membrane integrity rate, the acrosome integrity rate, the survival time and a certain motion parameter in the semen preservation period are detected by using a computer-aided sperm analyzer (CASA), a Coomassie brilliant blue staining method, a hypotonic test method and other methods; according to the diluent formula obtained by screening, the motility rate and the vitality are slowly and stably reduced in the semen preservation period, the time at the vitality of more than 0.6 can reach more than 3 days, andthe total survival time can reach more than 17 days; and a purpose of the invention is to objectively evaluate and screen out a formula of a diluent with the best Hu sheep semen normal-temperature preservation effect so as to provide reference for popularization and application of a Hu sheep semen preservation technology in production and further improve the breeding scale of Hu sheep.

Description

technical field [0001] The invention relates to a diluent formula for storing Hu sheep semen at room temperature and a dilution method thereof, belonging to the technical field of Hu sheep breeding. Background technique [0002] Hu sheep is a world-renowned breed of multiparous sheep. It has the advantages of four seasons of estrus, two births a year, and fast growth and development. Therefore, the scale of Hu sheep breeding is rapidly expanding. Artificial insemination technology is a basic technology in livestock breeding. It can make full use of the semen of good breeding males and is an important means to improve the reproductive efficiency and productivity of livestock. [0003] Artificial insemination technology combined with the application of estrus synchronization technology can reduce breeding costs, prevent the spread of diseases, and achieve large-scale breeding and management. Semen preservation is a key link in artificial insemination technology. Semen preser...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 李拥军张柳明王健冯云奎马金亮
Owner YANGZHOU UNIV
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