Unlock instant, AI-driven research and patent intelligence for your innovation.

Target gene for lung cancer diagnosis and treatment

A gene and lung cancer technology, applied in the field of lncRNA markers for lung cancer diagnosis and treatment, can solve the problems of losing the best time for surgery, poor survival and prognosis of lung squamous cell carcinoma patients, etc.

Inactive Publication Date: 2020-04-07
QINGDAO MEDINTELL BIOMEDICAL CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of specific oncogenic driver gene mutations in lung squamous cell carcinoma, patients with recurrent lung squamous cell carcinoma have few treatment options, resulting in poor survival and prognosis of lung squamous cell carcinoma patients (Zhang XW, Chen SY, Xue DW, etc. .Expression of Nemo-like kinase was increased and negatively correlated with the expression of TCF4 in lung cancers[J].Int JClin Exp Pathol,2015,8:15086-15092.), due to the lack of early tumor-specific identification of squamous cell carcinoma of the lung Markers are used to identify squamous cell carcinoma patients with high recurrence and high risk of death. Many patients with squamous cell carcinoma of the lung are diagnosed at an advanced stage and have lost the best period for surgery. Finally, the 5-year survival rate of patients with squamous cell carcinoma of the lung is only 7%. (Siege RL, Miller KD, and Jemal A. Cancer statistics, 2015 [J]. CA: a cancer journal for clinicians, 2015, 65(1): 5-29)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Target gene for lung cancer diagnosis and treatment
  • Target gene for lung cancer diagnosis and treatment
  • Target gene for lung cancer diagnosis and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1 QPCR detection of the expression of AC103718.1 in lung cancer

[0074] 1. Collect samples

[0075] 46 pairs of lung squamous cell carcinoma tissues and their corresponding paracancerous tissue samples were collected.

[0076] Inclusion criteria:

[0077] 1) The pathological diagnosis is lung squamous cell carcinoma, 2) The RNA expression level and clinicopathological information of the sample are complete.

[0078] Exclusion criteria:

[0079] 1) Suffering from other malignant tumors other than squamous cell carcinoma of the lung, 2) The case has received radiotherapy, chemotherapy and targeted drug therapy before specimen collection.

[0080] 2. RNA sample preparation and quantitative analysis

[0081] Use Trizol reagent to extract RNA from sample tissue, the steps are as follows:

[0082] Add 1mL Trizol to a glass homogenate bottle in an ultra-clean bench, weigh 50-100mg of tissue into the glass homogenate bottle, adjust the rotation speed to about 1500...

Embodiment 2

[0097] Example 2 Silencing of the AC103718.1 gene

[0098] 1. Cell culture

[0099] The lung squamous cell line H2170 cells were cultured in DMEM medium supplemented with 10% fetal bovine serum and P / S. For adherent growth of cells, place in conditions of 5% CO 2 , Cultivated in a constant temperature incubator with a humidity of 37°C.

[0100] 2. Design of siRNA

[0101] Design and synthesis of siRNA targeting AC103718.1 gene, siRNA-AC103718.1 and general negative control siRNA-NC were purchased from Shanghai Jima Gene Chemical Technology Co., Ltd. The sequence of siRNA against AC103718.1 is F: 5'-AAUUCACUCACAUUUCCAGUC-3' (SEQ ID NO.5); R: 5'-CUGGAAAUGUGAGUGAAUUCU-3' (SEQ ID NO.6) (siRNA1); sense strand: 5' -UUACAUGUAGUAACAUCUCCA-3'(SEQ ID NO.7), antisense strand: 5'-GAGAUGUUACUACAUGUAAGA-3'(SEQ ID NO.8)(siRNA2)

[0102] 3. Transfection

[0103] The day before transfection, the cells in the logarithmic growth phase were diluted to a density of 2×10 5 cells / ml, the dilu...

Embodiment 3

[0110] Example 3 Effect of AC103718.1 on lung cancer cells

[0111] 1) The effect of AC103718.1 on the proliferation of lung squamous cell carcinoma cells was detected by MTT method:

[0112] Wash the transfected cell lines with PBS, remove dead cells, digest with trypsin, prepare a single cell suspension, dilute the cell suspension, and adjust the density to 5×10 4 cells / ml, and then inoculate in 96-well culture plate, add 100 μl of cell suspension to each well, put the 96-well culture plate in 37°C, 5% CO 2 Continue culturing for 48 hours in a cell incubator, add 1×MTT to each well, continue culturing for 4 hours, discard the supernatant, add DMSO to each inner well, shake for 10 minutes, and use a microplate reader to measure each well at a wavelength of 450nm OD value of the well.

[0113] The results of MTT showed that the cell growth and proliferation rate of the transfected siRNA1 group (0.385±0.013) was significantly lower than that of the blank control group (0.598±...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a target gene for lung cancer diagnosis and treatment. The target gene is AC103718.1. The invention discloses application of AC103718.1 in lung squamous cell carcinoma. The invention discloses that the expression of AC103718.1 is significantly up-regulated in lung squamous carcinoma. For this reason, AC103718.1 can serve as a diagnostic marker for lung cancer to be used forjudging whether a subject has lung cancer. The invention also discloses that the siRNA aiming at AC103718.1 can affect the proliferation and migration of cancer cells, so that AC103718.1 can be usedas a drug target for the treatment of lung squamous carcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to lncRNA markers for diagnosis and treatment of lung cancer, and the specific marker is AC103718.1. Background technique [0002] Lung cancer is one of the leading causes of cancer-related death, and it is also the tumor with the highest incidence and highest mortality rate in the world. According to statistics from the American Cancer Society, there were about 234,030 new cases of lung cancer in the United States in 2018, and the number of deaths was about 154,050 For example, the 5-year overall survival rate is still poor (10-15%) (Siegel RL, Miller KD and Jemal A. Cancer statistics, 2019 [J]. CA Cancer JClin, 2019, 69(1): 7-34.), And its male patients are much higher than females. The high morbidity and mortality rate of lung cancer in cancer, the rapid progress of the disease, and the poor treatment effect not only seriously affect the living standards of people at home and abroad, but...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6886A61K45/00A61K31/713A61P35/00A61P11/00
CPCA61K31/713A61K45/00A61P11/00A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 杨承刚高舒欣黄露宁
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD