Hydrangea macrophylla-polar bear (a variety of hydrangea macrophylla) tissue culture medium and culture method

A tissue culture and culture method technology, applied in the medium and culture field of hydrangea-polar bear tissue culture, can solve the problems of low high-quality commodity rate and inability to reproduce quickly, achieve high commodity rate, improve reproduction speed and seedling uniformity , the effect of reducing production costs

Inactive Publication Date: 2020-04-10
WUXI MISHO ECOLOGY LANDSCAPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional breeding is limited by seasons, cannot reproduce in large numbers and quickly, and the rate of high-quality goods is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A cultivation method for hydrangea-polar bear tissue culture, comprising the following steps:

[0023] (1) Obtaining sterile materials: select explants with good growth and no tender branches of diseases and insect pests, remove the leaves of the explants, lightly brush to remove the surface fluff, soak in clean water with detergent for no more than 1 minute, and then rinse The explants were filtered until there was no foam; on the ultra-clean workbench, sterilized with a prepared mixed solution of 0.08% mercuric chloride and Tween for 10 minutes, rinsed with sterile water 4 times, filtered to dry, and sterilized The finished explants are cut off the wound and the injured part, cut into length 2cm stem segments with axillary buds, inserted into the prepared medium and cultivated for 9 days;

[0024] (2) Bud induction: the axillary bud induction medium is 1 / 2WPM+6-BA 1mg / L+2,4-D 0.1 mg / L, pH 5.8, temperature 24°C, light intensity 2300lx, light Alternate with dark cultur...

Embodiment 2

[0029] A cultivation method for hydrangea-polar bear tissue culture, comprising the following steps:

[0030] (1) Obtaining sterile materials: select explants with good growth and no tender branches of diseases and insect pests, remove the leaves of the explants, lightly brush to remove the surface fluff, soak in clean water with detergent for no more than 1 minute, and then rinse The explants were filtered until there was no foam; on the ultra-clean workbench, sterilized with a prepared mixed solution of 0.09% mercuric chloride and Tween for 6 minutes, rinsed with sterile water for 4 times, dried the water, and sterilized The finished explant is cut off the wound and the injured part, cut into a stem section with axillary buds of 2 cm in length, inserted into the prepared medium and cultivated for 8 days;

[0031] (2) Bud induction: the axillary bud induction medium is 1 / 2WPM+6-BA1.25 mg / L+2,4-D 0.1 mg / L, pH is 5.8, temperature is 25°C, light intensity is 2500lx , light and ...

Embodiment 3

[0036] A cultivation method for hydrangea-polar bear tissue culture, comprising the following steps:

[0037] (1) Obtaining sterile materials: select explants with good growth and no tender branches of diseases and insect pests, remove the leaves of the explants, lightly brush to remove the surface fluff, soak in clean water with detergent for no more than 1 minute, and then rinse The explants were filtered until there was no foam; on the ultra-clean workbench, sterilized with a prepared mixed solution of 0.1% mercuric chloride and Tween for 8 minutes, rinsed with sterile water for 6 times, drained the water, and sterilized The finished explant is cut off the wound and the injured part, cut into a stem segment with axillary buds of 2 cm in length, inserted into the prepared medium and cultivated for 7 days;

[0038] (2) Bud induction: the axillary bud induction medium is 1 / 2WPM+6-BA 1.5 mg / L+2,4-D 0.1 mg / L, pH is 5.8, temperature is 23°C, light intensity is 2700lx, Alternate ...

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Abstract

The present invention belongs to the technical field of plant tissue culture and particularly relates to a hydrangea macrophylla-polar bear (a variety of hydrangea macrophylla) tissue culture medium and culture method. The hydrangea macrophylla-polar bear tissue culture medium comprises an axillary bud induction medium, a proliferation medium and a rooting medium, wherein the axillary bud induction medium comprises 1-2 mg/L of (1/2WPM+6-BA) + 0.1 mg/L of 2,4-D, the proliferation medium comprises 1.6-2.0 mg/L of (1/2MS+6-BA) + 0.05 mg/L of IBA and the rooting medium comprises 0.8-1.2 mg/L of (1/2WPM+IBA). The tissue culture method comprises steps of acquisition of sterile materials, induction of buds, proliferation culture, rooting culture and seedling exercising. The tissue culture methodhas a high proliferation rate and a high commercial rate, is easy to operate, can greatly reduce production costs, breaks seasonal restrictions, and facilitates transportation.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a hydrangea-polar bear tissue culture medium and a culture method. [0002] technical background [0003] Hydrangea: It is a plant of the genus Hydrangea in the family Saxifragaceae, a shrub, 1-4 meters high; the stem often emits many radial branches at the base to form a circular bush; the branches are cylindrical, the leaves are papery or nearly leathery, obovate or broad Oval. Corymbose cymes subglobose, 8-20 cm in diameter, with short peduncles, dense flowers, pink, light blue or white; petals oblong, 3-3.5 mm long, immature capsules, long tops shape; seeds are immature, flowering period is June to August. [0004] Hydrangea has plump, large and beautiful flowers. Its color can be red or blue, which is pleasing to the eye and refreshing. It is a common potted ornamental flower and tree. Hydrangeas were cultivated in China earlier, and hydrangeas wer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 周敬锋李涛田星凯
Owner WUXI MISHO ECOLOGY LANDSCAPE
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