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Surface modification method of magnetic polymer microspheres

A technology of surface modification and magnetic microspheres, which is applied in the direction of microsphere preparation, chemical instruments and methods, microcapsule preparations, etc., and can solve the problems of low coupling efficiency and effect effects

Active Publication Date: 2020-04-17
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the surface of magnetic polymer microspheres obtained by this method has a small number of functional groups for biocoupling, resulting in low coupling efficiency for further coupling
In addition, due to the strong hydrophobicity of polymer materials, subsequent non-specific adsorption of proteins and other substances in biological samples will affect the effect of biological applications.

Method used

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  • Surface modification method of magnetic polymer microspheres
  • Surface modification method of magnetic polymer microspheres
  • Surface modification method of magnetic polymer microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The method of reacting PGMA-coated magnetic beads with cysteamine to obtain magnetic beads with amino groups on the surface:

[0051] 1. Suspend 1 gram of magnetic beads (1 micron in size) wrapped by PGMA in a mixed solvent of 5 mL of tetrahydrofuran / ethanol (1 / 20) to obtain a solution; wherein, the mixed solvent contains lithium hydroxide (4.2 mg, 0.10mmol);

[0052] 2. Slowly add cysteamine (154mg, 2mmol, SH-CH 2 -CH 2 -NH 2 ), and the reaction was stirred overnight at room temperature.

[0053] 3. After the reaction, use a magnet to attract the magnetic beads, and wash them twice in the order of ethanol, ultrapure water, 1X PBS solution and ultrapure water, and then vacuum-dry the washed magnetic beads at 30°C. Put it in a vacuum bag for storage, and you will get -NH on the surface 2 magnetic bead microspheres.

Embodiment 2

[0055] The magnetic beads of PGMA-coated magnetic beads react with ethylenediamine to obtain magnetic beads with amino groups on the surface:

[0056] 1. 0.8 grams of PGMA-wrapped magnetic beads (1 micron in size) are suspended in a mixed solvent of 4 mL of tetrahydrofuran / ethanol (1 / 20) to obtain a solution; wherein, the mixed solvent contains lithium hydroxide (4.2 mg, 0.10mmol);

[0057] 2. Slowly add ethylenediamine (96mg, 1.6mmol, NH 2 -CH 2 -CH 2 -NH 2 ), and the reaction was stirred overnight at room temperature.

[0058] 3. After the reaction, use a magnet to attract the magnetic beads, and wash them twice in the order of ethanol, ultrapure water, 1X PBS solution and ultrapure water, and then vacuum-dry the washed magnetic beads at 30°C. Put it in a vacuum bag for storage, and you will get -NH on the surface 2 magnetic bead microspheres.

Embodiment 3

[0060] The magnetic beads of PGMA-wrapped magnetic beads react with methyl 3-mercaptopropionate to obtain magnetic beads with carboxyl groups on the surface:

[0061] 1. 1.0 grams of PGMA-wrapped magnetic beads (1 micron in size) were suspended in a mixed solvent of 5 mL of tetrahydrofuran / ethanol (1 / 20) to obtain a solution; wherein, the mixed solvent also contained pyridine (8 μL, 0.10 mmol );

[0062] 2. Slowly add methyl 3-mercaptopropionate (240mg, 2mmol, SH-CH 2 -CH 2 -COOCH 3 ), stirred and reacted overnight at room temperature;

[0063] 3. After the reaction is over, use a magnet to absorb the magnetic beads, and wash them twice with a mixed solvent of tetrahydrofuran / ethanol (1 / 20) and ethanol;

[0064] 4. Suspend the washed magnetic beads again in 15 mL of tetrahydrofuran / ethanol (1 / 20) mixed solvent, which also contains lithium hydroxide (84 mg, 2 mmol), and react overnight at 50 ° C;

[0065] 5. After the reaction, use a magnet to attract the magnetic beads, a...

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Abstract

The invention provides a surface modification method of magnetic polymer microspheres. The surface modification method comprises the following steps: reacting epoxy groups on the surfaces of magneticpolymer microspheres with a primary amine or an ester with sulfydryl or amino to obtain magnetic bead microspheres with the amino or carboxyl on the surface; then carrying out branching amplificationon the introduced amino or carboxyl, and finally carrying out amidation reaction on the magnetic microspheres subjected to surface amino or carboxyl amplification and an amino acid derivative with ahydroxyl side chain so as to introduce a large amount of hydrophilic hydroxyl. According to the surface modification method, the magnetic microspheres with a large number of active groups (amino or carboxyl) and hydrophilic hydroxyl groups on the surfaces can be obtained, the magnetic microspheres can be efficiently coupled with bioactive substances, high hydrophilicity of the magnetic microspheres can be guaranteed, and non-characteristic adsorption during magnetic bead application after subsequent coupling is reduced. The invention further provides the magnetic microspheres subjected to surface modification.

Description

technical field [0001] The invention relates to the technical field of magnetic bead modification, in particular to a method for surface modification of magnetic polymer microspheres. Background technique [0002] Magnetic polymer microspheres are polymer-coated magnetic substances (such as Fe 3 o 4 ) formed a composite material with superparamagnetism, which can produce a magnetic response with the application and removal of an external magnetic field; and can achieve multiple functions by coupling biologically active substances, such as cell separation, immunopurification and detection, etc. has wide application. The surface of magnetic polymer microspheres can be chemically modified with required active functional groups, such as carboxyl, amino, hydroxyl, mercapto, etc., which provides the possibility for subsequent coupling of various biologically active substances. [0003] The most commonly used method to introduce active functional groups on the surface of magneti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02B01J20/26B01J20/30
CPCB01J13/02B01J20/06B01J20/26B01J20/261B01J2220/46B01J2220/4806B01J2220/4812
Inventor 玄曙光陈艳
Owner SHENZHEN INST OF ADVANCED TECH
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