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A kind of surface modification method of magnetic polymer microspheres

A technology of surface modification and magnetic microspheres, which is applied in the direction of microsphere preparation, chemical instruments and methods, microcapsule preparations, etc., which can solve the problems of low coupling efficiency and effect effects

Active Publication Date: 2021-11-26
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the surface of magnetic polymer microspheres obtained by this method has a small number of functional groups for biocoupling, resulting in low coupling efficiency for further coupling
In addition, due to the strong hydrophobicity of polymer materials, subsequent non-specific adsorption of proteins and other substances in biological samples will affect the effect of biological applications.

Method used

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  • A kind of surface modification method of magnetic polymer microspheres
  • A kind of surface modification method of magnetic polymer microspheres
  • A kind of surface modification method of magnetic polymer microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The method of reacting PGMA-coated magnetic beads with cysteamine to obtain magnetic beads with amino groups on the surface:

[0050] 1. Suspend 1 gram of magnetic beads (1 micron in size) wrapped by PGMA in a mixed solvent of 5 mL of tetrahydrofuran / ethanol (1 / 20) to obtain a solution; wherein, the mixed solvent contains lithium hydroxide (4.2 mg, 0.10mmol);

[0051] 2. Slowly add cysteamine (154mg, 2mmol, SH-CH 2 -CH 2 -NH 2 ), and the reaction was stirred overnight at room temperature.

[0052] 3. After the reaction, use a magnet to attract the magnetic beads, and wash them twice in the order of ethanol, ultrapure water, 1X PBS solution and ultrapure water, and then vacuum-dry the washed magnetic beads at 30°C. Put it in a vacuum bag for storage, and you will get -NH on the surface 2 magnetic bead microspheres.

Embodiment 2

[0054] The magnetic beads of PGMA-coated magnetic beads react with ethylenediamine to obtain magnetic beads with amino groups on the surface:

[0055] 1. 0.8 grams of PGMA-wrapped magnetic beads (1 micron in size) are suspended in a mixed solvent of 4 mL of tetrahydrofuran / ethanol (1 / 20) to obtain a solution; wherein, the mixed solvent contains lithium hydroxide (4.2 mg, 0.10mmol);

[0056] 2. Slowly add ethylenediamine (96mg, 1.6mmol, NH 2 -CH 2 -CH 2 -NH 2 ), and the reaction was stirred overnight at room temperature.

[0057] 3. After the reaction, use a magnet to attract the magnetic beads, and wash them twice in the order of ethanol, ultrapure water, 1X PBS solution and ultrapure water, and then vacuum-dry the washed magnetic beads at 30°C. Put it in a vacuum bag for storage, and you will get -NH on the surface 2 magnetic bead microspheres.

Embodiment 3

[0059] The magnetic beads of PGMA-wrapped magnetic beads react with methyl 3-mercaptopropionate to obtain magnetic beads with carboxyl groups on the surface:

[0060] 1. 1.0 grams of PGMA-wrapped magnetic beads (1 micron in size) were suspended in a mixed solvent of 5 mL of tetrahydrofuran / ethanol (1 / 20) to obtain a solution; wherein, the mixed solvent also contained pyridine (8 μL, 0.10 mmol );

[0061] 2. Slowly add methyl 3-mercaptopropionate (240mg, 2mmol, SH-CH 2 -CH 2 -COOCH 3 ), stirred and reacted overnight at room temperature;

[0062] 3. After the reaction is over, use a magnet to absorb the magnetic beads, and wash them twice with a mixed solvent of tetrahydrofuran / ethanol (1 / 20) and ethanol;

[0063] 4. Suspend the washed magnetic beads again in 15 mL of tetrahydrofuran / ethanol (1 / 20) mixed solvent, which also contains lithium hydroxide (84 mg, 2 mmol), and react overnight at 50 ° C;

[0064] 5. After the reaction, use a magnet to attract the magnetic beads, a...

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Abstract

The invention provides a method for surface modification of magnetic polymer microspheres, comprising: first reacting epoxy groups of magnetic polymer microspheres with epoxy groups on the surface with primary amines or esters with mercapto or amino groups to obtain surface Magnetic bead microspheres with amino or carboxyl groups; branched amplification of the introduced amino or carboxyl groups, and finally the amide reaction of the surface amino or carboxyl amplified magnetic microspheres with amino acid derivatives with hydroxyl side chains, In order to introduce a large number of hydrophilic hydroxyl groups. This surface modification method can obtain magnetic microspheres with a large number of active groups (amino or carboxyl groups) and hydrophilic hydroxyl groups on the surface, which can be efficiently coupled with biologically active substances and ensure high hydrophilicity, reducing Non-specific adsorption when magnetic beads are applied after subsequent coupling occurs. The invention also provides the surface-modified magnetic microsphere.

Description

technical field [0001] The invention relates to the technical field of magnetic bead modification, in particular to a method for surface modification of magnetic polymer microspheres. Background technique [0002] Magnetic polymer microspheres are polymer-coated magnetic substances (such as Fe 3 o 4 ) formed a composite material with superparamagnetism, which can produce a magnetic response with the application and removal of an external magnetic field; and can achieve multiple functions by coupling biologically active substances, such as cell separation, immunopurification and detection, etc. has wide application. The surface of magnetic polymer microspheres can be chemically modified with required active functional groups, such as carboxyl, amino, hydroxyl, mercapto, etc., which provides the possibility for subsequent coupling of various biologically active substances. [0003] The most commonly used method to introduce active functional groups on the surface of magneti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02B01J20/26B01J20/30
CPCB01J13/02B01J20/06B01J20/26B01J20/261B01J2220/46B01J2220/4806B01J2220/4812
Inventor 玄曙光陈艳
Owner SHENZHEN INST OF ADVANCED TECH
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