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High-flux micro-fluidic chip for treating and collecting crypthecodinium cohnii

A technology of Crypthecodinium kohlii and microfluidic chip, which is applied in the field of high-throughput microfluidic chip, can solve the problems of lack of operability, influence on experimental results, and large flow rate limitations, so as to improve the integration and reliability. Detection efficiency, improve capture efficiency, reduce the effect of operation process

Pending Publication Date: 2020-04-21
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still some shortcomings in the above device, mainly including: the sample solution has a single type of fluid, the flow rate is limited, and the cell capture efficiency is low; the formation of a stable micro-vortex requires a high flow rate, which is likely to cause deformation of the channel material and affect the experimental results; release, the concentration and purity of the solution collected in practical applications are low, which affects subsequent observation and further experiments; most of the existing microfluidic chips are only used as devices for cell sorting and enrichment, and the functions of the devices are single and relatively integrated. Difference
Simple application of existing microfluidic chip technology to realize the processing and collection of Cryptidium koirii cells will greatly increase the complexity of subsequent operations, and the precise control of cell processing cannot be achieved, and it does not have the operability of practical applications

Method used

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  • High-flux micro-fluidic chip for treating and collecting crypthecodinium cohnii
  • High-flux micro-fluidic chip for treating and collecting crypthecodinium cohnii
  • High-flux micro-fluidic chip for treating and collecting crypthecodinium cohnii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Such as figure 1 , 2 Shown in and 3 is a high-throughput microfluidic chip device for the treatment and collection of C. Liquid inlets and outlets and contraction-expansion structures are designed on the chip, and the main structures include: 1-sample cell suspension inlet in the microchannel system, 2-PBS buffer inlet in the microchannel system, 3-inlet of the microchannel system Straight channel, 4-expansion cavity in the microfluidic system, 5-sheath flow sample collection outlet in the microfluidic system, 6-waste outlet in the microfluidic system, sample inlet and sample outlet on the chip The ports are all connected to the outside world, which is convenient for connecting microtubes, loading or discharging liquid. The structural dimensions are designed as follows: the overall thickness of the microchannel is 50 μm, the size of the sample solution inlet and buffer inlet is 100 μm, the size of the sheath flow sample collection outlet and the waste liquid outlet is...

Embodiment 2

[0056] In this example, the structure of the high-throughput microfluidic chip used for the treatment and collection of Cryptidium koesii is basically the same as that of Example 1, except that the overall thickness of the microchannel is 30 μm, and the sample inlet, The size of the sheath inflow inlet is 50 μm, the size of the waste liquid outlet and the sample collection outlet is 50 μm, the number of sudden expansion chambers is 1, the size of the contraction section is 50 μm in width and 400 μm in length, and the size of the expansion section is 4 mm in width and 4 mm in length.

Embodiment 3

[0058] Using a high-throughput microfluidic chip for the treatment and collection of Cryptidium koirii, the capture and treatment of Cryptidium koirii cells is realized. First, an analytically pure polyethylene oxide (PEO) solution with a concentration of 300ppm was prepared. After the solution was prepared, it was stirred slowly with magnetic stirring (<30r / min) for 1h, and then placed in a shaker (100r / min) for 24h. Dilute 1 mL of Cryptidinium koirii cell suspension 10 times with 300 ppm PEO solution to obtain sample cell suspension.

[0059] Close the water stop clip of the sheath outlet, and open the water stop clip of the waste liquid outlet. The sample solution and buffer solution are sucked into the syringe, and injected into the microchannel from the sample solution inlet and the buffer solution inlet respectively by the syringe pump. The buffer solution was introduced first, and the initial volume flow rate was set to 600 μL / min. After the fluid fills the entire flo...

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Abstract

The invention discloses a high-flux micro-fluidic chip for treating and collecting crypthecodinium cohnii. The chip is provided with an asymmetric contraction-expansion type micro-fluid channel. One side of the channel is connected with two liquid inlets, and the other side is connected with an outlet. One side of a sudden expansion cavity is connected with a liquid outlet. According to the high-throughput micro-fluidic chip for treating and collecting crypthecodinium cohnii, capture of a sample cell suspension is realized in the micro-channel, cells are kept to rotate in the micro-vortex according to a fixed track, crypthecodinium cohnii cells are treated by utilizing shear stress and are collected through a sheath flow outlet in one side of the cavity, the operation is simple, and the shearing precision is high; and meanwhile, the chip can be integrated with observation of the motion state of the crypthecodinium cohnii cells after partial structures of the crypthecodinium cohnii cells are sheared and DHA yield research, the morphological structure and the motion state of the crypthecodinium cohnii cells after the cells are under the action of shearing force in the vortex for a certain time are researched, and the DHA yield of the crypthecodinium cohnii cells is increased.

Description

technical field [0001] The invention relates to a microfluidic analysis and detection technology in the field of biochemical engineering, in particular to a high-throughput microfluidic chip used for processing and collecting Cryptidium kourii. Background technique [0002] Achieving particle and cell capture in a simple, efficient, high-throughput, label-free method is of great significance in the fields of disease diagnosis, chemical and biological analysis, and microalgae biotechnology. Microfluidic technology mainly realizes the precise operation of fluids, particles, bubbles, droplets, etc. in the flow channel by building a micron-scale flow channel device. Since the cell microenvironment in the device can be controlled by adjusting different factors such as flow and shear stress, it can provide high-throughput cell manipulation and can precisely control the cells in the chamber. It has broad application prospects in cell capture and biochemical synthesis. In microflu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N1/12C12R1/89
CPCC12M23/16C12M45/02C12N1/12Y02A50/30
Inventor 朱国瑞卢钰文石鑫谭蔚
Owner TIANJIN UNIV
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