Insertion/deletion markers and method for identifying common colored foxes and arctic colored foxes
A technology of arctic foxes and colorful foxes, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., and can solve the problem of hybrid offspring not being able to reproduce, inability to distinguish fox species, and juvenile fox classification and identification difficulties and other problems, to achieve the effect of protecting germplasm resources, reliable test results, and rational selection and utilization
Inactive Publication Date: 2020-04-21
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
The body shape and appearance of the hybrid offspring are similar to arctic foxes, and the coat color is similar to that of the genus Vulpes. It is difficult to distinguish them by appearance, but the hybrid offspring do not have the ability to reproduce and cannot be reserved for breeding
There are still some difficulties in identifying the morphology of adult foxes, and it is even more difficult to classify and identify juvenile foxes
It is not possible to distinguish the populations of the genus Vulpes well, which will cause germplasm mixing and waste of resources
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0021] The present invention will be further described below in conjunction with specific examples, but is not limited thereto.
[0022] A molecular marker and a method for identifying common Vulpes and Arctic Vulpes of the present invention, specifically comprising the following steps:
[0023] a. Collection of samples of common Vulpes and Arctic Vulpes
[0024] The present invention uses red fox (n=31), silver black fox (n=29), chocolate fox (n=34), blue arctic fox (n=32) and white arctic fox (n=26) five kinds of fox populations as detection object
[0025] b. Extraction and homogenization of genomic DNA from blood samples
[0026] Use the blood genomic DNA extraction kit (catalogue number: DP348) produced by Tiangen Biochemical Technology (Beijing) Co., Ltd. to extract fox whole blood genomic DNA. The specific operation steps are as follows:
[0027] 1) Take 200 μL of fresh, frozen or anticoagulant-added blood and put it into a 1.5mL centrifuge tube;
[0028] 2) Add 200...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides insertion / deletion markers and a method for identifying common foxes and arctic foxes. The method comprises the following steps: extracting genome DNA; carrying out PCR amplification; carrying out DNA sequencing on a purified PCR product so as to determine insertion / deletion sites; and conducting genotype analysis through sequence comparison, and identifying fox groups according to genotype results. The method provided by the invention has the advantages of simple steps, high accuracy, good specificity and good repeatability, does not need special instruments, and can meet requirements of conventional laboratory detection. According to the method, germplasm mixing caused by natural mating and artificial propagation can be avoided, germplasm resources are protected, and seed selection and utilization are more reasonable.
Description
technical field [0001] The invention relates to the field of identification of common colored foxes belonging to the genus Vulpes and colored foxes belonging to the genus Vulpes. Background technique [0002] Insertion / deletion (insertion / deletion, indel) mutation refers to the insertion or deletion of nucleotide fragments of different sizes in the sequence of the same site in the genome between different individuals of the same species or between closely related species. At a certain site, one or more bases are inserted or deleted compared with another homologous sequence. Indel markers are molecular markers based on PCR amplification technology, which are essentially length polymorphic markers. Indel markers have many advantages, such as good stability, high polymorphism, and simple typing system, so they are now widely used in animal and plant breeding. [0003] Foxes are divided into the genus Vulpes and the genus Arctic Vulpes. The red fox in the genus Vulpes is the ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 彭永东李祥龙王瑞宁周瑞红赵子雅
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY