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Sealing film used in microorganism culture experiment and manufacturing method thereof

A technology of microbial culture and production method, applied in the field of parafilm used in microbial culture experiments, can solve the problems of inconvenient use, high temperature resistance, easy cracking, etc., and achieve the effect of convenient and quick use, high viscosity strength and stable structure

Inactive Publication Date: 2020-04-24
全保庆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, during the use of Parafilm PM996, the following defects were found: 1. Parafilm PM996 is an imported product from the United States, which is expensive; 2. It is very inconvenient to use, similar to the use of double-sided tape, which increases the workload of the experimenter ;3. Not resistant to high temperature, it will soften and become sticky at 68°C, and easy to crack when extended at 30°C; 4. No aseptic treatment is carried out during production and transportation, so it cannot be directly used for sterility test

Method used

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  • Sealing film used in microorganism culture experiment and manufacturing method thereof
  • Sealing film used in microorganism culture experiment and manufacturing method thereof
  • Sealing film used in microorganism culture experiment and manufacturing method thereof

Examples

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Embodiment 1

[0030] The preparation method of the parafilm used in the microbial culture experiment of the present embodiment comprises the following steps:

[0031] (1) While the three-layer co-extrusion extruder is working, the polyisobutylene is directly injected into the outer layer extruder and the inner layer extruder through the pressure conveying device to mix with polyethylene, and the addition temperature of the polyisobutylene is 110°C. The amount added is 6% of the weight of polyethylene, and then blown into a film;

[0032] (2) Sterilize the composite membrane with a hydrogen peroxide low-temperature plasma sterilizer in a closed environment at a sterilization temperature of 50° C., and then carry out sealed packaging in a sterile environment.

Embodiment 2

[0034] The preparation method of the parafilm used in the microbial culture experiment of the present embodiment comprises the following steps:

[0035] (1) While the three-layer co-extrusion extruder is working, the ethylene-vinyl acetate copolymer is directly injected into the outer layer extruder and the inner layer extruder through the pressure conveying device to mix with polyvinyl chloride, and the ethylene-vinyl acetate copolymer The addition temperature of the copolymer is 125°C, the addition amount is 5% of the weight of polyvinyl chloride, and then blown into a film;

[0036] (2) Sterilize the composite membrane with a hydrogen peroxide low-temperature plasma sterilizer in a closed environment at a sterilization temperature of 50° C., and then carry out sealed packaging in a sterile environment.

Embodiment 3

[0038] The preparation method of the parafilm used in the microbial culture experiment of the present embodiment comprises the following steps:

[0039] (1) While the three-layer co-extrusion extruder is working, the polyisobutylene is directly injected into the outer layer extruder and the inner layer extruder through the pressure conveying device to mix with polyvinyl chloride, and the addition temperature of polyisobutylene is 130°C , the added amount is 8% of the weight of polyvinyl chloride, and then blown into a film;

[0040] (2) Sterilize the composite membrane with a hydrogen peroxide low-temperature plasma sterilizer in a closed environment at a sterilization temperature of 45° C., and then carry out sealed packaging in a sterile environment.

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Abstract

The embodiment of the invention discloses a sealing film used in a microorganism culture experiment and a manufacturing method of the sealing film, and belongs to the technical field of experiment consumables. The sealing film is prepared from matrix resin and a tackifier as raw materials through blow molding, sterilization and packaging. The sealing film disclosed by the embodiment of the invention is stable in structure, can be normally used in a temperature range of -20 DEG C to 55 DEG C, is high in viscous strength, and can firmly lock moisture in a culture medium after sealing, so that the influence on microorganism culture is reduced to the minimum. The sealing film disclosed by the embodiment of the invention has the advantages of low cost, convenience and quickness in use, no peculiar smell, safety and capability of being directly used for a sterile test, and can replace Parafilm PM996 to be used as the sealing film of experimental articles such as culture dishes, test tubes and dryers.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of experimental consumables, and in particular to a parafilm used in microorganism cultivation experiments and a manufacturing method thereof. Background technique [0002] U.S. Parafilm (article number: PM996) is a thermoplastic self-sealing, moldable, and tough special film product with unique permeability, excellent water vapor permeability and strong corrosion resistance. It is widely used in conventional Laboratory, especially microbial culture experiments. [0003] However, during the use of Parafilm PM996, the following defects were found: 1. Parafilm PM996 is an imported product from the United States, which is expensive; 2. It is very inconvenient to use, similar to the use of double-sided tape, which increases the workload of the experimenter ; 3. Not resistant to high temperature, it will soften and become sticky at 68°C, and easy to crack when extended at 30°C; 4. No as...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08L23/06C08L27/06C08L27/16C08L23/20C08L23/08C12M1/00
CPCC08J5/18C12M1/00C08J2323/06C08J2327/06C08J2327/16C08J2423/20C08J2423/08C08J2423/06
Inventor 全保庆
Owner 全保庆
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