Recombinant yeast strain, and construction method and application thereof

Abstract

The invention provides a construction method of a recombinant yeast strain. The construction method comprises the following steps: synthesizing a plurality of heterologous promoters; constructing a beta-carotene gene expression module, wherein the beta-carotene gene expression module comprises a beta-carotene production related gene, the promoter at the upstream of the beta-carotene production related gene and a terminator at the downstream of the beta-carotene production related gene; and converting the beta-carotene gene expression module into Saccharomyces cerevisiae to obtain the recombinant yeast. The recombinant yeast strain obtained by the construction method contains the various high-activity heterologous promoters, so that the Saccharomyces cerevisiae has the characteristics of high yield and stability. The invention also provides a recombinant yeast strain and an application thereof.

Description

technical field [0001] The invention relates to the field of recombinant yeast strains and its construction method and application. Background technique [0002] β-carotene has important physiological functions. It is a precursor substance for the synthesis of human vitamin A. It can enhance human immunity and prevent various diseases such as arteriosclerosis and cancer. It has anti-oxidation and anti-aging activities and is widely used in Drugs, cosmetics, food and health products, etc., so it has a very large market value. [0003] Saccharomyces cerevisiae is a food-grade and safe microorganism with simple and easy-to-operate genome and short fermentation cycle. It is an ideal chassis cell for the synthesis of β-carotene. [0004] It is believed in the prior art that the key enzymes involved in the biosynthesis of β-carotene include tetraprenyl pyrophosphate synthase (CrtE / BTS1), lycopene dehydrogenase (CrtI), lycopene synthase / lycopene cyclase (CrtYB), etc., and the exp...

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Problems solved by technology

[0004] It is believed in the prior art that the key enzymes involved in the biosynthesis of β-carotene include tetraprenyl pyrophosphate synthase (CrtE / BTS1), lycopene dehydrogenase (CrtI), lycopene synthase / lycopene cyclase (CrtYB), etc., and the expression of the above key enzymes depends on various promoters in the yeast genome, promoters with low activity will lead to low production of β-carotene, and β-carotene The biosynthesis of carotene involves the joint action of multiple genes, so that some promoters are repeatedly used for gene expression, which may lead to the instability of the fermentation of recombinant strains

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