Bactrocera dorsalis attractant and preparation method thereof
A Bactrocera dorsalis attractant and Bactrocera dorsalis technology are applied in the direction of attracting pests, botanical equipment and methods, biocides, etc., to achieve the effects of reducing egg laying damage, benefiting ecological environment protection and sustainable development
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Embodiment 1
[0031] Embodiment 1 Liaoshixin volatile oil extract preparation
[0032] Liao Asarum medicinal material (screened to remove sediment) is cut into pieces, 250g is put into a distillation flask, soaked in water for 1 hour, heated at about 100v to boil and distilled for 5 hours to obtain a distillation solution, and the precipitated essential oil part is separated with a separatory funnel, and packed into an essential oil bottle. About 3ml of essential oil can be obtained per 500g of Liaoshixin original drug, and each 6ml of essential oil can be used as an experimental sample.
Embodiment 2
[0033] Example 2 Preliminary Identification of Compounds in Liao Asarum Volatile Oil Extract
[0034] 1. GC-MS analysis
[0035] GC-MS analysis conditions use gas chromatography-mass spectrometry for Agilent7890B-5977A.
[0036]1. Chromatographic conditions: Chromatographic column: Agilent 19091s-433HP-5MS, 5% benzyl polysiloxane elastic quartz capillary column (30m×250μm 0.25μm); inlet temperature is 260°C; temperature program: initial temperature 70°C, maintain 0min, then rise to 75°C at 1°C·min-1, maintain for 5min; then rise to 107°C at 8°C·min-1, maintain 0min; continue to rise to 125°C at 50°C·min-1 , maintained for 5 minutes; then raised to 160°C at 10°C·min-1 and maintained for 3 minutes; finally raised to 240°C at 8°C·min-1 and maintained for 4 minutes. Carrier gas: He, flow rate 30mL·min-1, split ratio 20:1, injection volume 1μL, Gassaver: off.
[0037] 2. Mass spectrometry conditions: interface temperature: 260°C, ionization method: EI, electron energy: 70eV, ion...
Embodiment 3
[0051] The fresh-keeping boxes of the test group and the control group were placed separately at each test point for 5 days, and the two fresh-keeping boxes were adjacent to each other. After 5 days, record and count the Bactrocera dorsalis quantity (seeing table 2) that lures in each fresh-keeping box.
[0052]
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