Alpha-L-fucosidase determination reagent

A fucosidase and reagent technology, applied in the field of medical testing, can solve the problems of increased absorbance of reagent blanks, unstable substrates, and insufficient sensitivity of kits, etc.

Pending Publication Date: 2020-05-01
桂林英美特生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The continuous monitoring method has the characteristics of rapid testing and convenient operation. The α-L-fucosidase assay kit based on this has been gradually popularized and applied in cli

Method used

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  • Alpha-L-fucosidase determination reagent
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  • Alpha-L-fucosidase determination reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0022] Reagent components:

[0023]

[0024] Dissolve each component in purified water in turn, mix well, adjust the pH value between 4.5 and 6.0 with 1mmol / L hydrochloric acid solution or sodium hydroxide solution, and then dilute to 1L with purified water.

Embodiment example 2

[0026] Reagent components:

[0027]

[0028]

[0029] Dissolve each component in purified water in turn, mix well, adjust the pH value between 4.5 and 6.0 with 1mmol / L hydrochloric acid solution or sodium hydroxide solution, and then dilute to 1L with purified water.

Embodiment example 3

[0031] Reagent components:

[0032]

[0033] Dissolve each component in purified water in turn, mix well, adjust the pH value between 4.5 and 6.0 with 1mmol / L hydrochloric acid solution or sodium hydroxide solution, and then dilute to 1L with purified water.

[0034] In the thermal stability test of the reagents of the above three embodiments placed at 37°C for 7 days and the storage stability test of 12 months at a storage temperature of 2 to 8°C, the M-G-2-chloro-4-nitrate in the reagent There is no obvious hydrolysis phenomenon of phenyl-α-L-fucoside, and no obvious change in the appearance of the reagent. When detecting according to the assay method disclosed in the present invention, the upper limit of the linear range of the assay α-L-fucosidase of the reagent can reach 200U / L.

[0035] An α-L-fucosidase assay reagent of the present invention, said α-L-fucosidase assay reagent comprises 80-200mmol / L buffer solution, 5-10g / L sodium chloride, 0.1-1g / L L color source-f...

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Abstract

The present invention discloses an alpha-L-fucosidase determination reagent. The alpha-L-fucosidase determination reagent comprises 80-200 mmol/L of a buffer, 5-10 g/L of sodium chloride, 0.1-1 g/L ofa color source-fucoside substrate, 0.5-2 g/L of a stabilizer, 0.1-2 g/L of an activator, 0.5-5 g/L of a non-ionic surfactant, and 2-5 g/L of a metal complexing agent. The stabilizer is added to solvethe problem of significant increase of a reagent blank caused by a hydrolysis reaction of the color source-fucoside substrate, and the GOOD'S biological buffer and the activator are used to improve stability and sensitivity.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to an alpha-L-fucosidase assay reagent. Background technique [0002] α-L-fucosidase (a-L-Fucosidase, AFU) is a lysosomal acid hydrolase, classified as a-L-fucosidase fucosidase (EC32.1.51). The basic physiological function of AFU is to catalyze the catabolism of macromolecular substances such as various glycolipids, mucopolysaccharides, glycoproteins and glycosides containing fucosyl groups. It is widely distributed in various tissues, cells and body fluids in the human body. Studies have confirmed that AFU is a new tumor marker for the diagnosis of primary liver cancer. With the further in-depth research on AFU, the detection methods are also continuously developed. The main detection methods of AFU include fluorescence method, chemical colorimetric method, continuous monitoring method and so on. The substrate of the fluorescence method is 4-methylumbelliferone α-L-fuc...

Claims

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Application Information

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IPC IPC(8): C12Q1/34
CPCC12Q1/34
Inventor 俸小雄黄艳妮
Owner 桂林英美特生物技术研究所
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