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Traditional Chinese medicine monomer composition for anti-inflammation

A technology of monomer composition and traditional Chinese medicine, applied in the direction of drug combination, anti-inflammatory agent, antipyretic, etc., can solve the problems of anti-inflammatory pharmacological effects and research reports of traditional Chinese medicine monomer composition, and achieve good anti-inflammation in vivo Effects of activity, prolonging survival time, good medicinal prospects

Pending Publication Date: 2020-05-05
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that forsythin, emodin, baicalin, and liquiritin are the main anti-inflammatory active ingredients of forsythia, rhubarb, baicalin, and licorice, respectively (Chinese Medicine Pharmacology and Clinic, 2007, 23:51-52; Chinese Herbal Medicine, 1999, 7: 522-524; Acta Pharmaceutica Sinica, 2000, 3:161-164; Chinese Journal of Traditional Chinese Medicine, 2013, 38:99-104), but so far there is no research report on the anti-inflammatory pharmacological effect of this traditional Chinese medicine monomer composition

Method used

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  • Traditional Chinese medicine monomer composition for anti-inflammation
  • Traditional Chinese medicine monomer composition for anti-inflammation
  • Traditional Chinese medicine monomer composition for anti-inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Design of Orthogonal Test Table

[0026] According to the non-toxic doses of forsythin, emodin, baicalin, and glycyrrhizin screened in the previous stage, orthogonal design was performed to obtain the orthogonal test design table of Chinese medicine monomer composition (Table 1).

[0027] Table 1 Orthogonal test design table of non-toxic doses of forsythin, emodin, baicalin, and glycyrrhizin composition

[0028]

[0029] Note: In the following examples, the following marks are used to represent the concentration of each component.

[0030] A: 1=0.4μM, 2=0.2μM, 3=0.1μM; B: 1=4μM, 2=2μM, 3=1μM;

[0031] C: 1=20 μM, 2=10 μM, 3=5 μM; D: 1=50 μM, 2=25 μM, 3=12.5 μM.

Embodiment 2

[0032] Example 2 Toxicity evaluation test of forsythin, emodin, baicalin and glycyrrhizin composition to zebrafish

[0033] Tg (coro1a: eGFP; lyz: Dsred) zebrafish juveniles were randomly divided into groups (n=30, 3day postfertilization), which were the drug group and the control group. The control group was given the same concentration of dimethyl sulfoxide (DMSO) and soaked Administration, observe and record zebrafish survival rate and deformity rate (spinal curvature, pericardial cyst, skeletal deformity) within 72 hours.

[0034] The mortality and distortion rate of zebrafish juveniles are calculated as follows:

[0035] Survival rate = number of surviving zebrafish embryos / total zebrafish embryos×100%

[0036] Abnormality rate = number of abnormal zebrafish embryos / total zebrafish embryos×100%

[0037] The results of the test did not cause the effects of the composition of forsythin, emodin, baicalin, and glycyrrhizin to cause distortion and death of zebrafish juveniles, that is,...

Embodiment 3

[0040] Example 3 Protective effect of forsythin, emodin, baicalin, glycyrrhizin composition on zebrafish death caused by lipopolysaccharide (LPS)

[0041] Test method: Tg (coro1a: eGFP; lyz: Dsred) zebrafish juveniles were randomly divided into groups (n=30, 3daypost fertilization), yolk sac was microinjected with 2mg / mL LPS to create a model, and then immersed in forsythin and emodin. , Baicalin, glycyrrhizin composition or 5μg / mL dexamethasone (DEX) treatment, yolk sac microinjection of phosphate buffered saline (PBS) as a negative control group, observe and record the survival of zebrafish within 72h, and draw Survival curve.

[0042] Test results: Orthogonal analysis shows that the composition of forsythin, emodin, baicalin, and glycyrrhizin can all reduce the death of zebrafish caused by LPS (Table 3).

[0043] Further orthogonal test analysis of variance showed that four monomers of forsythin, emodin, baicalin, and glycyrrhizin are indispensable for the activity of the composi...

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PUM

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Abstract

The invention discloses a traditional Chinese medicine monomer composition for anti-inflammation. The traditional Chinese medicine monomer composition mainly consists of the following raw materials inmolar ratio: 0.1-0.4 of emodin, 1-4 of baicalin, 5-20 of forsythin and 12.5-50 of liquiritin. The invention also discloses an application of the traditional Chinese medicine monomer composition for anti-inflammation in preparation of a pharmaceutical preparation with an anti-inflammation effect. The Chinese medicinal monomer composition has good in vivo anti-inflammatory activity, can obviously prolong the survival time of animals with inflammation, and has a good medicinal prospect.

Description

Technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to an anti-inflammatory traditional Chinese medicine monomer composition and its application. Background technique [0002] Forsythia, rhubarb, scutellaria and licorice are commonly used Chinese medicines. Forsythia suspensa is the dried fruit of the Oleaceae plant Forsythia suspensa, which has the functions of clearing heat and detoxifying, reducing swelling and dispersing knots, and dispersing wind heat. Rhubarb is the dried roots and rhizomes of Polygonaceae plant Rheum palmatum, Rheum tanguticum or medicinal rhubarb. It has the functions of purging and convulsing, clearing heat and fire, stopping bleeding, detoxification, promoting blood circulation and removing blood stasis, and clearing damp heat. Scutellaria baicalensis Georgi is used as medicine with the dried roots of Scutellaria baicalensis Georgi, a plant of the Lamiaceae family, which has the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61K31/7048A61P29/00
CPCA61K31/122A61K31/7048A61P29/00A61K2300/00
Inventor 余林中刘俊珊刘东依卢子滨郑远茹周红玲
Owner SOUTHERN MEDICAL UNIVERSITY