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Method for preparing dumbbell-shaped composite nanomaterials using magnetosomes

A technology of composite nanomaterials and magnetosomes, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as environmental pollution and difficulty in preparing nanocomposites, and achieve simple and easy preparation methods Good effect with high application value

Active Publication Date: 2021-02-05
BEIJING GUO KE RONG ZHI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the preparation of dumbbell-shaped nanocomposites is relatively difficult. Most of them are prepared by chemical methods, which require high-temperature reactors and catalysis of flammable and explosive organic solvents. The requirements for experimental equipment and operators are relatively strict, and it is easy to cause environmental damage. Pollution

Method used

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  • Method for preparing dumbbell-shaped composite nanomaterials using magnetosomes
  • Method for preparing dumbbell-shaped composite nanomaterials using magnetosomes
  • Method for preparing dumbbell-shaped composite nanomaterials using magnetosomes

Examples

Experimental program
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Embodiment 1

[0049] Example 1 Preparation of dumbbell-shaped magnetosomes-silver composite nanomaterials (1)

[0050] This embodiment provides a method for preparing a dumbbell-shaped magnetosome-silver composite nanomaterial, specifically as follows:

[0051] (1) Submerged fermenter culture of magnetotactic bacteria

[0052] The wild-type Magnetospirillum gryphiswaldense MSR-1 (MSR-WT) was cultured in a fermenter for large collection of bacteria and purification of magnetosomes: strains stored at -80°C were inoculated in fresh In the sodium lactate medium, cultured at 30°C and 100rpm for 24 hours; two generations of continuous activation, and the third generation of seed liquid was used for fermenter culture. References for fermenter culture conditions Zhang Y, Zhang X, Jiang W, Li Y, Li J.Semicontinuousculture of Magnetospirillum gryphiswaldense MSR-1 cells in an autofermentorby nutrient-balanced and isosmotic feeding strategies. Appl Environ Microbiol. 2011;77(17):5851-5856. During th...

Embodiment 2

[0056] Example 2 Preparation of dumbbell-shaped magnetosomes-silver composite nanomaterials (2)

[0057] This embodiment provides a method for preparing a dumbbell-shaped magnetosome-silver composite nanomaterial, the difference from Example 1 is that the magnetotactic bacteria used in step (1) is the MamF knockout mutation of Magnetospirillum gryphiswaldense MSR-1 strain (MSR-ΔF).

Embodiment 3

[0058] Example 3 Preparation of dumbbell-shaped magnetosomes-silver composite nanomaterials (3)

[0059] This embodiment provides a method for preparing a dumbbell-shaped magnetosome-silver composite nanomaterial, the only difference from Example 1 is that the magnetotactic bacteria used in step (1) is the MamC knockout mutation of Magnetospirillum gryphiswaldense MSR-1 strain (MSR-ΔC).

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Abstract

The invention relates to the technical field of biomaterials, in particular to a method for preparing dumbbell-shaped composite nanomaterials using magnetosomes. The invention finds that the magnetosome can interact with noble metal ions such as silver and gold, so that the noble metal ion generates nanoparticles on the specific crystal face of the magnetosome, and prepares dumbbell-shaped composite nanomaterials. The present invention prepares dumbbell-shaped magnetosome composite nanomaterials by incubating magnetosomes with metal salt solution. The preparation method has high application value and is suitable for wide popularization and application.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to the application of magnetosomes in preparing dumbbell-shaped composite nanomaterials and a method for preparing dumbbell-shaped magnetosomes-noble metal composite nanomaterials using magnetosomes. Background technique [0002] Magnetosomes (bacterial magnetic nanoparticles, BMPs) are magnetic nanoparticles synthesized in vivo by magnetotactic bacteria and coated with a unit membrane, mainly composed of Fe 3 o 4 or Fe 3 S 4 composition. The outer unit membrane is called the magnetosome membrane, which contains a variety of membrane proteins related to the synthesis of magnetosomes. Magnetotactic bacteria are widely distributed in nature, and there are various types, with spiral, rod, spherical and multicellular forms. The crystal forms and sizes of magnetosomes synthesized by different species of magnetotactic bacteria are different. Magnetospirillum gryphiswaldense MSR...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01G49/08C01G49/12C01G7/00C01G5/00C12P3/00C12R1/01
CPCC01G5/00C01G7/00C01G49/08C01G49/12C01P2004/04C01P2004/80C12P3/00
Inventor 田杰生徐俊杰
Owner BEIJING GUO KE RONG ZHI BIOTECH CO LTD