Method for extracting polyphenol compounds from coltsfoot flowers
A technology of polyphenolic compounds and extraction methods, which is applied in the field of preparation of active ingredients of traditional Chinese medicine, can solve the problems of large pollution, strong volatility, and flammability of organic reagents, and achieve the effects of strong volatility, simple steps, and low cost
Active Publication Date: 2020-05-08
SHANXI UNIV
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Problems solved by technology
[0005] The purpose of the present invention is to address above-mentioned existing problems, provide a kind of method utilizing novel extraction solvent to extract the polyphenol compound in Coltsfoot flower; Explosion and other issues
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Embodiment 1
[0024] 1. Mix 28g of choline chloride and 36.8g of glycerin (molar ratio 1:2), heat in a water bath at 80°C until a uniform and transparent solution is formed, which is the extraction solvent;
[0025] 2. Weigh 10 grams of coltsfoot flower, add 60 g of the above extraction solvent, ultrasonically extract for 40 minutes, centrifuge at 3500 rpm for 5 minutes, and take the supernatant;
[0026] 3. Soak the HP20 macroporous resin in 95% ethanol overnight and load it into a column. The volume of the column is 100mL. Rinse it with distilled water until there is no alcohol smell. Take the supernatant obtained above and load it with 0.1% formic acid water 1000mL and 50% Elute with 1000mL of ethanol, collect 50% ethanol eluate, concentrate and dry to obtain polyphenol compounds of coltsfoot flower.
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The invention provides a method for extracting polyphenol compounds from coltsfoot flowers and relates to the field of preparation of traditional Chinese medicine effective ingredients. The method forextracting the polyphenol compounds comprises the following steps: (1) placing choline chloride and glycerine, which are in a mole ratio of 1: 2, in water bath of 80 DEG C, so as to synthesize an extraction solvent; (2) adding the coltsfoot flowers into the extraction solvent according to a mass ratio of 1: 6, carrying out ultrasonic extraction for 40min, carrying out centrifugation, and then, taking supernatant; and (3) eluting the supernatant through macroporous resin HP-20 to remove the extraction solvent, carrying out eluting sequentially with 0.1% formic acid water and 50% ethanol solution, collecting an eluting cut of the 50% ethanol, and carrying out concentrating, thereby obtaining the polyphenol compounds of the coltsfoot flowers. The method has the advantages that the polyphenolcompounds can be selectively extracted and prepared in one step; and the method is simple in step and low in cost, and the problems that organic reagents are heavy in pollution and high in volatilityand are combustible and explosive are avoided.
Description
technical field [0001] The invention relates to the preparation of active ingredients of traditional Chinese medicines, in particular to a method for extracting polyphenol compounds from coltsfoot flowers by using a novel extraction solvent. [0002] technical background [0003] Coltsfoot flower is the dry flower bud of the plant Coltsfoot Tussilagofafara L. in Compositae. It has the effects of moistening the lungs, relieving cough and reducing phlegm. It is mainly used clinically for cough, asthma, and anti-inflammation. The chemical composition of coltsfoot flower includes flavonoids, sterols, phenolic acids, alkaloids, volatile oils, sesquiterpenes, etc. Among them, flavonoids and phenolic acids belong to polyphenolic compounds. Preliminary studies have shown that these ingredients have the effect of reducing phlegm and relieving cough (Journal of Pharmaceutical and Biomedical Analysis, 2013, 75:158-164), which has the prospect of further development of new drugs. [000...
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IPC IPC(8): A61K36/28
CPCA61K36/28A61K2236/333A61K2236/55
Inventor 李震宇李蓉蓉秦雪梅
Owner SHANXI UNIV