Unlock instant, AI-driven research and patent intelligence for your innovation.

Reagent for detecting and targeting at RP11-754B17.1 and application of reagent in arthritis

A technology for osteoarthritis and reagents, which is applied in the field of reagents for detecting and targeting biomarker RP11-754B17.1, and can solve the problems that osteoarthritis cannot be cured, there is no effective treatment, and the impact on patients' families and society

Active Publication Date: 2020-05-08
XIAN HONGHUI HOSPITAL
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Osteoarthritis cannot be cured at present, and there is no effective treatment after the onset, which has a serious impact on patients, their families and society

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent for detecting and targeting at RP11-754B17.1 and application of reagent in arthritis
  • Reagent for detecting and targeting at RP11-754B17.1 and application of reagent in arthritis
  • Reagent for detecting and targeting at RP11-754B17.1 and application of reagent in arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 QPCR sequencing to verify the expression of RP11-754B17.1 gene

[0055] 1. Sample collection

[0056] A total of 78 patients undergoing knee replacement surgery were selected, including 48 OA patients and 30 normal controls. Blood samples and tissue samples were collected from the patients. The age and gender of the OA group and the control group were not statistically significant.

[0057] OA group: Inclusion criteria: 1) Osteoarthritis patients diagnosed according to OA diagnostic criteria, underwent knee replacement surgery; 2) Initial replacement. Exclusion criteria: 1) Patients with other inflammatory arthritis or autoimmune diseases, including rheumatoid arthritis, gout, systemic lupus erythematosus, etc.; 2) Patients with a history of severe trauma to the knee joint; 3) Patients with steroids within the past 3 months Those with a history of drug injection or application of non-steroidal drugs; 4) those with severe liver and kidney function diseases and...

Embodiment 2

[0068] Example 2 Silencing of RP11-754B17.1 gene and its influence on cells

[0069] 1. Cell acquisition and culture

[0070] Wash the cartilage tissue of OA patients with 1% double-antibody PBS repeatedly for 3 times, and cut the tissue to 1mm with sterile small ophthalmic scissors 3 Put the cartilage fragments into 10cm Petri dish, add 10ml of fresh 0.25% trypsin, 37℃, 5%CO 2Digest in the incubator for 30min to remove possible attached fibroblasts. Aspirate the trypsin digestion solution, add 10ml of DMEM culture solution containing 10% FBS, pipette and mix well to stop the digestion, wash twice with 10ml of PBS containing double antibody. Add 10ml of 0.2% type II collagenase to the petri dish, place at 37°C, 5% CO 2 Digest for 4 hours in the incubator. The dissociated chondrocyte suspension containing collagenase solution was sucked out with a pipette, filtered through a 70 μm 200-mesh cell sieve, the obtained filtrate was transferred to a centrifuge tube and centrifuge...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a reagent for detecting and targeting at RP11-754B17.1 and application of the reagent in arthritis. The invention finds that RP11-754B17.1 is capable of effectively distinguishing patients suffering from bone arthritis from normal people, and meanwhile, cell proliferation can be promoted and cell apoptosis can be reduced by reducing the expression level of RP11-754B17.1, sothat a novel molecular means is provided for diagnosis and treatment on bone arthritis.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a reagent for detecting and targeting biomarker RP11-754B17.1 and its application in arthritis. Background technique [0002] Osteoarthritis (OA) is a heterogeneous chronic disease with extremely complex pathogenic factors, which has brought a huge economic burden to the society. OA has a very high disability rate and seriously reduces the quality of life of patients (Hunter DJ, Schofield D, Callander E. The individual and socioeconomic impact of osteoarthritis[J]. Nat Rev Rheumatol, 2014, 10(7): 437-441 .), but there is still no effective treatment. When OA occurs, a series of pathological changes occur in the joint tissue: including cartilage degradation, synovial inflammation, osteophyte formation, and subchondral bone sclerosis (Moon PM, Beier F. Novel Insights into Osteoarthritis Joint Pathology from Studies in Mice[J].Curr Rheumatol Rep, 2015, 17(8):50). Cartilage erosion is a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883A61K45/00A61K31/7088A61P19/02A61P29/00
CPCA61K31/7088A61K45/00A61P19/02A61P29/00C12Q1/6883C12Q2600/136C12Q2600/158
Inventor 马涛王军宋伟程辉光张育民
Owner XIAN HONGHUI HOSPITAL