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Application of epo in anti-Staphylococcus aureus infection drugs

A technique for Staphylococcus infection and Staphylococcus, which is applied in the field of biomedicine to achieve the effect of reducing the number of neutrophils, reducing the dosage, and improving the degree of infiltration

Active Publication Date: 2022-07-12
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, vancomycin, which is considered to be the last line of defense against Gram-positive bacteria, has also been challenged

Method used

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  • Application of epo in anti-Staphylococcus aureus infection drugs
  • Application of epo in anti-Staphylococcus aureus infection drugs
  • Application of epo in anti-Staphylococcus aureus infection drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Example 1 In vitro study of the promoting effect of EPO on macrophage phagocytosis of Staphylococcus aureus

[0039] By adding PBS or EPO to macrophages, or selecting mouse macrophages from different sources, the phagocytic ability of macrophages to Staphylococcus aureus was detected. Thus, it was verified that EPO could promote the phagocytosis of Staphylococcus aureus by macrophages in vitro.

[0040] 1. Animals:

[0041] C57 / BL6 mice, male, 8-10 weeks old, weighing 18-20 g, were purchased from the Experimental Animal Center of the Third Military Medical University, and were normally kept in a clean animal room. Macrophage EPOR conditional knockout mice (prepared by our laboratory), 8-10 weeks old, weighing 18-20 grams, were raised in SPF animal room.

[0042] 2. Experimental grouping

[0043] PBS group: wild-type mouse macrophages, treated with PBS

[0044] rhEPO group: wild-type mouse macrophages, treated with rhEPO

[0045] WT group: wild-type mouse macrophages...

Embodiment 2

[0051] Example 2 In vivo study of EPO combined with vancomycin in the treatment of Staphylococcus aureus infection

[0052] The mice were infected and inflammatory by injecting Staphylococcus aureus into skin vesicles. They were given different treatments and divided into groups. Then the number of neutrophils in the skin cysts, the number of Staphylococcus aureus colonies and the levels of inflammatory factors were detected. The skin of the mice was observed. Tissue inflammatory cell infiltration to verify that EPO combined with vancomycin in the treatment of Staphylococcus aureus infection can reduce the dosage of vancomycin.

[0053] 1. Animals:

[0054] C57 / BL6 mice, male, 8-10 weeks old, weighing 18-20 g, were purchased from the Experimental Animal Center of the Third Military Medical University, and were normally kept in a clean animal room.

[0055] 2. Experimental grouping

[0056] PBS group: PBS treatment

[0057] rhEPO group: treated with rhEPO 5000IU / kg

[0058]...

Embodiment 3

[0063] Example 3 In vitro study of the promoting effect of EPO on the phagocytosis of methicillin-resistant Staphylococcus aureus by macrophages

[0064] By adding PBS or EPO to macrophages, or selecting mouse macrophages from different sources, the phagocytic ability of macrophages to methicillin-resistant Staphylococcus aureus was detected. Thus, it was verified that EPO could promote the phagocytosis of methicillin-resistant Staphylococcus aureus by macrophages in vitro.

[0065] 1. Animals:

[0066] C57 / BL6 mice, male, 8-10 weeks old, weighing 18-20 g, were purchased from the Experimental Animal Center of the Third Military Medical University, and were normally kept in a clean animal room. Macrophage EPOR conditional knockout mice (prepared by our laboratory), 8-10 weeks old, weighing 18-20 grams, were raised in SPF animal room.

[0067] 2. Experimental grouping

[0068] PBS group: wild-type mouse macrophages, treated with PBS

[0069] rhEPO group: wild-type mouse macr...

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Abstract

The invention belongs to the technical field of biomedicine, in particular to the application of EPO and its analogs in the preparation of medicines for treating Staphylococcus aureus infection and a method for promoting macrophage phagocytosis of Staphylococcus aureus in vitro. By adding EPO and its analogs to macrophages, promoting macrophage phagocytosis of Staphylococcus aureus can effectively combat Staphylococcus aureus infection. The present invention provides a new application of EPO and its analogs, and reveals that activation of EPOR in macrophages can promote the phagocytosis of Staphylococcus aureus by macrophages, especially the phagocytosis of methicillin-resistant Staphylococcus aureus.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to the application of EPO and its analogs in the preparation of medicines for treating Staphylococcus aureus infection and a method for promoting macrophage phagocytosis of Staphylococcus aureus in vitro. Background technique [0002] Staphylococcus aureus is a Gram-positive coccus belonging to the genus Staphylococcus and is a common sexually pathogenic microorganism. According to the US Centers for Disease Control, infections caused by Staphylococcus aureus are second only to Escherichia coli. Because S. aureus is able to colonize multiple species, it can easily spread from one species to another, from humans to animals, and back again. Bacteria can spread from person to person through direct contact and direct contact with contaminated objects such as cell phones, telephones, doorknobs, faucets, computer keyboards, mice, knives, currency, and medical equipment. It may even ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61K38/08A61P31/04
CPCA61K38/1816A61K38/08A61P31/04Y02A50/30
Inventor 张志仁梁飞红罗邦伟关惠婷
Owner ARMY MEDICAL UNIV
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