Wheat carotenoid synthesis route key gene Lcye mutant and application thereof

A technology of lcye-d1 and gene, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of unclear gene function and genetic regulation mechanism, restricting the progress of breeding work, etc.

Active Publication Date: 2020-05-19
CROP RES INST SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the current research on wheat Lcye gene is limited to QTL mapping, gene cloning and molecular marker development, an

Method used

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  • Wheat carotenoid synthesis route key gene Lcye mutant and application thereof
  • Wheat carotenoid synthesis route key gene Lcye mutant and application thereof
  • Wheat carotenoid synthesis route key gene Lcye mutant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Functional research on the key gene Lcye of wheat carotenoid synthesis pathway

[0042] 1. Materials and methods

[0043] 1. EMS mutagenesis population construction

[0044] The construction of the EMS mutagenesis population refers to the method of Slade et al. (2005). First, a batch of stable homozygous Jimai 20 with consistent size and plump grains was screened (Ma Shaokang et al. Effects of different water and nitrogen treatments on the protein components and processed varieties of Jimai 20. Journal of Wheat Crops, 2010, 30(3) :477-481) and Jimai 22 (Liu Jia et al. Changes in flag leaf chlorophyll and active oxygen scavenging enzyme activity of high-yield wheat variety Jimai 22. Shandong Agricultural Science, 2012,44(8):31-34) seeds, Utilize the chemical mutagen EMS (Solarbio, E8150) of 1.2% concentration to carry out mutagenesis treatment, produce a series of point mutations; Plant the seed greenhouse after treatment to obtain mutant population M 1 Gen...

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Abstract

The invention discloses a wheat carotenoid synthesis route key gene Lcye mutant and an application thereof. The invention provides the following proteins: a protein obtained by replacing the 253th-site serine of a Lcye-D1 protein with phenylalanine, or a derived protein which is obtained through replacement and/or deletion and/or addition of one or several amino acid residues on the Lcye-D1 protein and has the same capacity as the Lcye-D1 protein, or a protein which as 99% or above, 95% or above, 90% or above, 85% or above or 80% or above of homology with an amino acid sequence restricted by the Lcye-D1 protein and has the same functions as the Lcye-D1 protein, or a fusion protein which is obtained through connecting a label with an N end and/or a C end of the Lcye-D1 protein. According tothe wheat carotenoid synthesis route key gene Lcye mutant disclosed by the invention, the functions of a Lcye gene are verified, and a theory basis and germplasm resources are also provided for colorproperty improvement of flour and products thereof.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a mutant of the key gene Lcye in the synthesis pathway of wheat carotenoids and an application thereof. Background technique [0002] Flour color is an important sensory index and market index to evaluate the quality of wheat flour. Yellow pigment is the most important natural pigment in wheat grain, and it is the main reason for the yellowness of flour and its products. The correlation coefficients between grain yellow pigment content and flour and dough yellowness are as high as 0.8-0.9, and the correlation coefficients with bread and noodle color are 0.69 and 0.76 (Mares and Campbell, 2001; Adom et al., 2003; Fratianni et al., 2005; Cong et al., 2010). Chinese noodles, such as white noodles, steamed buns, steamed buns and dumplings, have relatively high requirements on the whiteness of flour, and fine and white flour and its products are very popular. [0003] Carotenoids are t...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/46
CPCC07K14/415C12N15/8261C12Y505/01018C12N9/90C12N15/8243C12N15/825
Inventor 翟胜男刘建军李豪圣宋健民刘爱峰曹新有程敦公赵振东刘成郭军韩冉訾妍李法计汪晓璐
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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