The lcye mutant of the key gene of wheat carotenoid synthesis pathway and its application

A technology of lcye-d1 and gene, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of unclear gene function and genetic regulation mechanism, restricting the progress of breeding work, etc.

Active Publication Date: 2022-02-01
CROP RES INST SHANDONG ACAD OF AGRI SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the current research on wheat Lcye gene is limited to QTL mapping, gene cloning and molecular marker development, and its gene function and genetic regulation mechanism are still unclear, which seriously restricts the progress of breeding work.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The lcye mutant of the key gene of wheat carotenoid synthesis pathway and its application
  • The lcye mutant of the key gene of wheat carotenoid synthesis pathway and its application
  • The lcye mutant of the key gene of wheat carotenoid synthesis pathway and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Functional research on the key gene Lcye of wheat carotenoid synthesis pathway

[0042] 1. Materials and methods

[0043] 1. EMS mutagenesis population construction

[0044] The construction of the EMS mutagenesis population refers to the method of Slade et al. (2005). First, a batch of stable homozygous Jimai 20 with consistent size and plump grains was screened (Ma Shaokang et al. Effects of different water and nitrogen treatments on the protein components and processed varieties of Jimai 20. Journal of Wheat Crops, 2010, 30(3) :477-481) and Jimai 22 (Liu Jia et al. Changes in flag leaf chlorophyll and active oxygen scavenging enzyme activity of high-yield wheat variety Jimai 22. Shandong Agricultural Science, 2012,44(8):31-34) seeds, Utilize the chemical mutagen EMS (Solarbio, E8150) of 1.2% concentration to carry out mutagenesis treatment, produce a series of point mutations; Plant the seed greenhouse after treatment to obtain mutant population M 1 Gen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a mutant of the key gene Lcye in the synthetic pathway of wheat carotenoid and its application. The present invention provides the following protein: the protein obtained by replacing the serine at position 253 of the Lcye-D1 protein with phenylalanine, or by substituting and / or deleting and / or adding one or several amino acid residues and a derivative protein with the same ability, or a protein with more than 99%, more than 95%, more than 90%, more than 85% or more than 80% homology and the same function with its defined amino acid sequence, or at its N-terminal And / or the fusion protein obtained after the C-terminus is linked with a tag. The invention not only verifies the function of the Lcye gene, but also provides a theoretical basis and germplasm resources for improving the color properties of flour and its products.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a mutant of the key gene Lcye in the synthesis pathway of wheat carotenoids and an application thereof. Background technique [0002] Flour color is an important sensory index and market index to evaluate the quality of wheat flour. Yellow pigment is the most important natural pigment in wheat grain, and it is the main reason for the yellowness of flour and its products. The correlation coefficients between grain yellow pigment content and flour and dough yellowness are as high as 0.8-0.9, and the correlation coefficients with bread and noodle color are 0.69 and 0.76 (Mares and Campbell, 2001; Adom et al., 2003; Fratianni et al., 2005; Cong et al., 2010). Chinese noodles, such as white noodles, steamed buns, steamed buns and dumplings, have relatively high requirements on the whiteness of flour, and fine and white flour and its products are very popular. [0003] Carotenoids are t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/46
CPCC07K14/415C12N15/8261C12Y505/01018C12N9/90C12N15/8243C12N15/825
Inventor 翟胜男刘建军李豪圣宋健民刘爱峰曹新有程敦公赵振东刘成郭军韩冉訾妍李法计汪晓璐
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap